In vitro techniques and genetic engineering of Veratrum californicum Duran

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

The genus Veratrum is distributed throughout the northern temperate and Arctic regions of Europe, Asia and North America. Veratrum californicum Duran (Melanthiaceae) is an important monocotyledonous medicinal plant which is the only source of the anticancer compound cyclopamine. In order to be able to utilize Veratrum cells in the production of important secondary metabolites, an in vitro platform is needed. For that purpose, tissue culture, green plant regeneration and genetic engineering of Veratrum californicum were developed. Fine suspension cell lines were established from germinated mature embryos by transferring friable embryogenic calli to AA- and L2-medium. The suspension cells were cryopreserved successfully and recovered at a high rate. In addition, a highly embryogenic and regenerating cell line was established and kept on L2-medium without additional growth regulators. Very good growth was obtained by employing the temporary immersion system of RITA -bioreactors. The highly regenerating cell line and in vitro plantlets contained steroid alkaloids cyclopamine and veratramine. Furthermore, transformation methods were developed for Veratrum californicum. Transgenic cell lines were produced by Agrobacterium tumefaciens and A. rhizogenes -mediated deliveries. Also direct gene transfer techniques such as particle bombardment and protoplast-based techniques were applied. The basic tools for the metabolic engineering and biotechnological production of secondary metabolites of Veratrum californicum are now available.
Original languageEnglish
Title of host publicationPlants for Human Health in the Post-Genome Era
Subtitle of host publicationPSE Congress
Place of PublicationEspoo
PublisherVTT Technical Research Centre of Finland
Pages88-88
ISBN (Electronic)978-951-38-6322-7
ISBN (Print)978-951-38-6321-0
Publication statusPublished - 2007
EventPSE Congress: Plants for Human Health in the Post-Genome Era - Helsinki, Finland
Duration: 26 Aug 200729 Aug 2007

Publication series

NameVTT Symposium
PublisherVTT
Number249
ISSN (Print)0357-9387
ISSN (Electronic)1445-0873

Conference

ConferencePSE Congress: Plants for Human Health in the Post-Genome Era
CountryFinland
CityHelsinki
Period26/08/0729/08/07

Fingerprint

Veratrum californicum
genetic engineering
Veratrum
cell lines
secondary metabolites
steroid alkaloids
Melanthiaceae
metabolic engineering
Rhizobium rhizogenes
bioreactors
Agrobacterium radiobacter
methodology
growth regulators
gene transfer
embryo (plant)
cell suspension culture
protoplasts
medicinal plants
tissue culture
Arctic region

Cite this

Ritala, A., Rischer, H., Ma, R., & Oksman-Caldentey, K-M. (2007). In vitro techniques and genetic engineering of Veratrum californicum Duran. In Plants for Human Health in the Post-Genome Era: PSE Congress (pp. 88-88). [C2] Espoo: VTT Technical Research Centre of Finland. VTT Symposium, No. 249
Ritala, Anneli ; Rischer, Heiko ; Ma, R. ; Oksman-Caldentey, Kirsi-Marja. / In vitro techniques and genetic engineering of Veratrum californicum Duran. Plants for Human Health in the Post-Genome Era: PSE Congress. Espoo : VTT Technical Research Centre of Finland, 2007. pp. 88-88 (VTT Symposium; No. 249).
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abstract = "The genus Veratrum is distributed throughout the northern temperate and Arctic regions of Europe, Asia and North America. Veratrum californicum Duran (Melanthiaceae) is an important monocotyledonous medicinal plant which is the only source of the anticancer compound cyclopamine. In order to be able to utilize Veratrum cells in the production of important secondary metabolites, an in vitro platform is needed. For that purpose, tissue culture, green plant regeneration and genetic engineering of Veratrum californicum were developed. Fine suspension cell lines were established from germinated mature embryos by transferring friable embryogenic calli to AA- and L2-medium. The suspension cells were cryopreserved successfully and recovered at a high rate. In addition, a highly embryogenic and regenerating cell line was established and kept on L2-medium without additional growth regulators. Very good growth was obtained by employing the temporary immersion system of RITA -bioreactors. The highly regenerating cell line and in vitro plantlets contained steroid alkaloids cyclopamine and veratramine. Furthermore, transformation methods were developed for Veratrum californicum. Transgenic cell lines were produced by Agrobacterium tumefaciens and A. rhizogenes -mediated deliveries. Also direct gene transfer techniques such as particle bombardment and protoplast-based techniques were applied. The basic tools for the metabolic engineering and biotechnological production of secondary metabolites of Veratrum californicum are now available.",
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Ritala, A, Rischer, H, Ma, R & Oksman-Caldentey, K-M 2007, In vitro techniques and genetic engineering of Veratrum californicum Duran. in Plants for Human Health in the Post-Genome Era: PSE Congress., C2, VTT Technical Research Centre of Finland, Espoo, VTT Symposium, no. 249, pp. 88-88, PSE Congress: Plants for Human Health in the Post-Genome Era, Helsinki, Finland, 26/08/07.

In vitro techniques and genetic engineering of Veratrum californicum Duran. / Ritala, Anneli; Rischer, Heiko; Ma, R.; Oksman-Caldentey, Kirsi-Marja.

Plants for Human Health in the Post-Genome Era: PSE Congress. Espoo : VTT Technical Research Centre of Finland, 2007. p. 88-88 C2 (VTT Symposium; No. 249).

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

TY - CHAP

T1 - In vitro techniques and genetic engineering of Veratrum californicum Duran

AU - Ritala, Anneli

AU - Rischer, Heiko

AU - Ma, R.

AU - Oksman-Caldentey, Kirsi-Marja

PY - 2007

Y1 - 2007

N2 - The genus Veratrum is distributed throughout the northern temperate and Arctic regions of Europe, Asia and North America. Veratrum californicum Duran (Melanthiaceae) is an important monocotyledonous medicinal plant which is the only source of the anticancer compound cyclopamine. In order to be able to utilize Veratrum cells in the production of important secondary metabolites, an in vitro platform is needed. For that purpose, tissue culture, green plant regeneration and genetic engineering of Veratrum californicum were developed. Fine suspension cell lines were established from germinated mature embryos by transferring friable embryogenic calli to AA- and L2-medium. The suspension cells were cryopreserved successfully and recovered at a high rate. In addition, a highly embryogenic and regenerating cell line was established and kept on L2-medium without additional growth regulators. Very good growth was obtained by employing the temporary immersion system of RITA -bioreactors. The highly regenerating cell line and in vitro plantlets contained steroid alkaloids cyclopamine and veratramine. Furthermore, transformation methods were developed for Veratrum californicum. Transgenic cell lines were produced by Agrobacterium tumefaciens and A. rhizogenes -mediated deliveries. Also direct gene transfer techniques such as particle bombardment and protoplast-based techniques were applied. The basic tools for the metabolic engineering and biotechnological production of secondary metabolites of Veratrum californicum are now available.

AB - The genus Veratrum is distributed throughout the northern temperate and Arctic regions of Europe, Asia and North America. Veratrum californicum Duran (Melanthiaceae) is an important monocotyledonous medicinal plant which is the only source of the anticancer compound cyclopamine. In order to be able to utilize Veratrum cells in the production of important secondary metabolites, an in vitro platform is needed. For that purpose, tissue culture, green plant regeneration and genetic engineering of Veratrum californicum were developed. Fine suspension cell lines were established from germinated mature embryos by transferring friable embryogenic calli to AA- and L2-medium. The suspension cells were cryopreserved successfully and recovered at a high rate. In addition, a highly embryogenic and regenerating cell line was established and kept on L2-medium without additional growth regulators. Very good growth was obtained by employing the temporary immersion system of RITA -bioreactors. The highly regenerating cell line and in vitro plantlets contained steroid alkaloids cyclopamine and veratramine. Furthermore, transformation methods were developed for Veratrum californicum. Transgenic cell lines were produced by Agrobacterium tumefaciens and A. rhizogenes -mediated deliveries. Also direct gene transfer techniques such as particle bombardment and protoplast-based techniques were applied. The basic tools for the metabolic engineering and biotechnological production of secondary metabolites of Veratrum californicum are now available.

M3 - Conference abstract in proceedings

SN - 978-951-38-6321-0

T3 - VTT Symposium

SP - 88

EP - 88

BT - Plants for Human Health in the Post-Genome Era

PB - VTT Technical Research Centre of Finland

CY - Espoo

ER -

Ritala A, Rischer H, Ma R, Oksman-Caldentey K-M. In vitro techniques and genetic engineering of Veratrum californicum Duran. In Plants for Human Health in the Post-Genome Era: PSE Congress. Espoo: VTT Technical Research Centre of Finland. 2007. p. 88-88. C2. (VTT Symposium; No. 249).