In vivo detection of vascular adhesion protein-1 in experimental inflammation

Kimmo Jaakkola, Tuomo Nikula, Riikka Holopainen, Tommi Vähäsilta, Marja-Terttu Matikainen, Marja-Leena Laukkanen, Risto Huupponen, Lauri Halkola, Lauri Nieminen, Jukka Hiltunen, Sakari Parviainen, Michael Clark, Juhani Knuuti, Timo Savunen, Pekka Kääpä, Liisa Voipio-Pulkki, Sirpa Jalkanen

Research output: Contribution to journalArticleScientificpeer-review

90 Citations (Scopus)

Abstract

Vascular adhesion protein-1 (VAP-1) is an inflammation-inducible endothelial glycoprotein which mediates leukocyte-endothelial cell interactions. To study the pathogenetic significance of VAP-1 in inflammatory disorders, an in vivo immunodetection method was used to detect the regulation of luminally expressed VAP-1 in experimental skin and joint inflammation in the pig and dog. Moreover, VAP-1 was studied as a potential target to localize inflammation by radioimmunoscintigraphy. Up-regulation of VAP-1 in experimental dermatitis and arthritis could be visualized by specifically targeted immunoscintigraphy. Moreover, the translocation of VAP-1 to the functional position on the endothelial surface was only seen in inflamed tissues. These results suggest that VAP-1 is both an optimal candidate for anti-adhesive therapy and a potential target molecule for imaging inflammation.

Original languageEnglish
Pages (from-to)463 471
Number of pages9
JournalAmerican Journal of Pathology
Volume157
Issue number2
DOIs
Publication statusPublished - 2000
MoE publication typeA1 Journal article-refereed

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Blood Vessels
Inflammation
Proteins
Radioimmunodetection
Experimental Arthritis
Dermatitis
Cell Communication
Adhesives
Glycoproteins
Leukocytes
Up-Regulation
Swine
Endothelial Cells
Joints
Dogs
Skin

Cite this

Jaakkola, K., Nikula, T., Holopainen, R., Vähäsilta, T., Matikainen, M-T., Laukkanen, M-L., ... Jalkanen, S. (2000). In vivo detection of vascular adhesion protein-1 in experimental inflammation. American Journal of Pathology, 157(2), 463 471. https://doi.org/10.1016/S0002-9440(10)64558-0
Jaakkola, Kimmo ; Nikula, Tuomo ; Holopainen, Riikka ; Vähäsilta, Tommi ; Matikainen, Marja-Terttu ; Laukkanen, Marja-Leena ; Huupponen, Risto ; Halkola, Lauri ; Nieminen, Lauri ; Hiltunen, Jukka ; Parviainen, Sakari ; Clark, Michael ; Knuuti, Juhani ; Savunen, Timo ; Kääpä, Pekka ; Voipio-Pulkki, Liisa ; Jalkanen, Sirpa. / In vivo detection of vascular adhesion protein-1 in experimental inflammation. In: American Journal of Pathology. 2000 ; Vol. 157, No. 2. pp. 463 471.
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title = "In vivo detection of vascular adhesion protein-1 in experimental inflammation",
abstract = "Vascular adhesion protein-1 (VAP-1) is an inflammation-inducible endothelial glycoprotein which mediates leukocyte-endothelial cell interactions. To study the pathogenetic significance of VAP-1 in inflammatory disorders, an in vivo immunodetection method was used to detect the regulation of luminally expressed VAP-1 in experimental skin and joint inflammation in the pig and dog. Moreover, VAP-1 was studied as a potential target to localize inflammation by radioimmunoscintigraphy. Up-regulation of VAP-1 in experimental dermatitis and arthritis could be visualized by specifically targeted immunoscintigraphy. Moreover, the translocation of VAP-1 to the functional position on the endothelial surface was only seen in inflamed tissues. These results suggest that VAP-1 is both an optimal candidate for anti-adhesive therapy and a potential target molecule for imaging inflammation.",
author = "Kimmo Jaakkola and Tuomo Nikula and Riikka Holopainen and Tommi V{\"a}h{\"a}silta and Marja-Terttu Matikainen and Marja-Leena Laukkanen and Risto Huupponen and Lauri Halkola and Lauri Nieminen and Jukka Hiltunen and Sakari Parviainen and Michael Clark and Juhani Knuuti and Timo Savunen and Pekka K{\"a}{\"a}p{\"a} and Liisa Voipio-Pulkki and Sirpa Jalkanen",
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Jaakkola, K, Nikula, T, Holopainen, R, Vähäsilta, T, Matikainen, M-T, Laukkanen, M-L, Huupponen, R, Halkola, L, Nieminen, L, Hiltunen, J, Parviainen, S, Clark, M, Knuuti, J, Savunen, T, Kääpä, P, Voipio-Pulkki, L & Jalkanen, S 2000, 'In vivo detection of vascular adhesion protein-1 in experimental inflammation', American Journal of Pathology, vol. 157, no. 2, pp. 463 471. https://doi.org/10.1016/S0002-9440(10)64558-0

In vivo detection of vascular adhesion protein-1 in experimental inflammation. / Jaakkola, Kimmo; Nikula, Tuomo; Holopainen, Riikka; Vähäsilta, Tommi; Matikainen, Marja-Terttu; Laukkanen, Marja-Leena; Huupponen, Risto; Halkola, Lauri; Nieminen, Lauri; Hiltunen, Jukka; Parviainen, Sakari; Clark, Michael; Knuuti, Juhani; Savunen, Timo; Kääpä, Pekka; Voipio-Pulkki, Liisa; Jalkanen, Sirpa.

In: American Journal of Pathology, Vol. 157, No. 2, 2000, p. 463 471.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - In vivo detection of vascular adhesion protein-1 in experimental inflammation

AU - Jaakkola, Kimmo

AU - Nikula, Tuomo

AU - Holopainen, Riikka

AU - Vähäsilta, Tommi

AU - Matikainen, Marja-Terttu

AU - Laukkanen, Marja-Leena

AU - Huupponen, Risto

AU - Halkola, Lauri

AU - Nieminen, Lauri

AU - Hiltunen, Jukka

AU - Parviainen, Sakari

AU - Clark, Michael

AU - Knuuti, Juhani

AU - Savunen, Timo

AU - Kääpä, Pekka

AU - Voipio-Pulkki, Liisa

AU - Jalkanen, Sirpa

PY - 2000

Y1 - 2000

N2 - Vascular adhesion protein-1 (VAP-1) is an inflammation-inducible endothelial glycoprotein which mediates leukocyte-endothelial cell interactions. To study the pathogenetic significance of VAP-1 in inflammatory disorders, an in vivo immunodetection method was used to detect the regulation of luminally expressed VAP-1 in experimental skin and joint inflammation in the pig and dog. Moreover, VAP-1 was studied as a potential target to localize inflammation by radioimmunoscintigraphy. Up-regulation of VAP-1 in experimental dermatitis and arthritis could be visualized by specifically targeted immunoscintigraphy. Moreover, the translocation of VAP-1 to the functional position on the endothelial surface was only seen in inflamed tissues. These results suggest that VAP-1 is both an optimal candidate for anti-adhesive therapy and a potential target molecule for imaging inflammation.

AB - Vascular adhesion protein-1 (VAP-1) is an inflammation-inducible endothelial glycoprotein which mediates leukocyte-endothelial cell interactions. To study the pathogenetic significance of VAP-1 in inflammatory disorders, an in vivo immunodetection method was used to detect the regulation of luminally expressed VAP-1 in experimental skin and joint inflammation in the pig and dog. Moreover, VAP-1 was studied as a potential target to localize inflammation by radioimmunoscintigraphy. Up-regulation of VAP-1 in experimental dermatitis and arthritis could be visualized by specifically targeted immunoscintigraphy. Moreover, the translocation of VAP-1 to the functional position on the endothelial surface was only seen in inflamed tissues. These results suggest that VAP-1 is both an optimal candidate for anti-adhesive therapy and a potential target molecule for imaging inflammation.

U2 - 10.1016/S0002-9440(10)64558-0

DO - 10.1016/S0002-9440(10)64558-0

M3 - Article

VL - 157

SP - 463 471

JO - American Journal of Pathology

JF - American Journal of Pathology

SN - 0002-9440

IS - 2

ER -

Jaakkola K, Nikula T, Holopainen R, Vähäsilta T, Matikainen M-T, Laukkanen M-L et al. In vivo detection of vascular adhesion protein-1 in experimental inflammation. American Journal of Pathology. 2000;157(2):463 471. https://doi.org/10.1016/S0002-9440(10)64558-0