In vivo detection of vascular adhesion protein-1 in experimental inflammation

Kimmo Jaakkola, Tuomo Nikula, Riikka Holopainen, Tommi Vähäsilta, Marja-Terttu Matikainen, Marja-Leena Laukkanen, Risto Huupponen, Lauri Halkola, Lauri Nieminen, Jukka Hiltunen, Sakari Parviainen, Michael Clark, Juhani Knuuti, Timo Savunen, Pekka Kääpä, Liisa Voipio-Pulkki, Sirpa Jalkanen

Research output: Contribution to journalArticleScientificpeer-review

98 Citations (Scopus)

Abstract

Vascular adhesion protein-1 (VAP-1) is an inflammation-inducible endothelial glycoprotein which mediates leukocyte-endothelial cell interactions. To study the pathogenetic significance of VAP-1 in inflammatory disorders, an in vivo immunodetection method was used to detect the regulation of luminally expressed VAP-1 in experimental skin and joint inflammation in the pig and dog. Moreover, VAP-1 was studied as a potential target to localize inflammation by radioimmunoscintigraphy. Up-regulation of VAP-1 in experimental dermatitis and arthritis could be visualized by specifically targeted immunoscintigraphy. Moreover, the translocation of VAP-1 to the functional position on the endothelial surface was only seen in inflamed tissues. These results suggest that VAP-1 is both an optimal candidate for anti-adhesive therapy and a potential target molecule for imaging inflammation.

Original languageEnglish
Pages (from-to)463 471
Number of pages9
JournalAmerican Journal of Pathology
Volume157
Issue number2
DOIs
Publication statusPublished - 2000
MoE publication typeA1 Journal article-refereed

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