In vivo synthesis of complex N-glycans by expression of human N-acetylglucosaminyltransferase I in the filamentous fungus Trichoderma reesei

Marleen Maras; André De Bruyn; Wouter Vervecken; Jaana Uusitalo; Merja Penttilä; Roger Busson; Piet Herdewijn; Roland Contreras

doi:10.1016/S0014-5793(99)00648-1

In vivo synthesis of complex N-glycans by expression of human N-acetylglucosaminyltransferase I in the filamentous fungus Trichoderma reesei

Marleen Maras, André De Bruyn, Wouter Vervecken, Jaana Uusitalo, Merja Penttilä, Roger Busson, Piet Herdewijn, Roland Contreras

Research output: Contribution to journal › Article › Scientific › peer-review

34 Citations (Scopus)

Abstract

The human N-acetylglucosaminyltransferase I gene was introduced in the genome of Trichoderma reesei strain VTT-D-80133. Expression was studied after induction from the cellobiohydrolase I promoter. Successful in vivo transfer of GlcNAc was demonstrated by analyzing the neutral N-glycans which were synthesized on cellobiohydrolase I. Final proof of the formation of GlcNAcMan₅GlcNAc₂ was obtained by NMR analysis. Copyright (C) 1999 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	365-370
Number of pages	6
Journal	FEBS Letters
Volume	452
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(99)00648-1
Publication status	Published - 11 Jun 1999
MoE publication type	A1 Journal article-refereed

Keywords

Cellobiohydrolase I
Fungus
N-acetylglucosaminyltransferase I
Trichoderma reesei

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10.1016/S0014-5793(99)00648-1

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title = "In vivo synthesis of complex N-glycans by expression of human N-acetylglucosaminyltransferase I in the filamentous fungus Trichoderma reesei",

abstract = "The human N-acetylglucosaminyltransferase I gene was introduced in the genome of Trichoderma reesei strain VTT-D-80133. Expression was studied after induction from the cellobiohydrolase I promoter. Successful in vivo transfer of GlcNAc was demonstrated by analyzing the neutral N-glycans which were synthesized on cellobiohydrolase I. Final proof of the formation of GlcNAcMan5GlcNAc2 was obtained by NMR analysis. Copyright (C) 1999 Federation of European Biochemical Societies.",

keywords = "Cellobiohydrolase I, Fungus, N-acetylglucosaminyltransferase I, Trichoderma reesei",

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doi = "10.1016/S0014-5793(99)00648-1",

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T1 - In vivo synthesis of complex N-glycans by expression of human N-acetylglucosaminyltransferase I in the filamentous fungus Trichoderma reesei

AU - Maras, Marleen

AU - De Bruyn, André

AU - Vervecken, Wouter

AU - Uusitalo, Jaana

AU - Penttilä, Merja

AU - Busson, Roger

AU - Herdewijn, Piet

AU - Contreras, Roland

PY - 1999/6/11

Y1 - 1999/6/11

N2 - The human N-acetylglucosaminyltransferase I gene was introduced in the genome of Trichoderma reesei strain VTT-D-80133. Expression was studied after induction from the cellobiohydrolase I promoter. Successful in vivo transfer of GlcNAc was demonstrated by analyzing the neutral N-glycans which were synthesized on cellobiohydrolase I. Final proof of the formation of GlcNAcMan5GlcNAc2 was obtained by NMR analysis. Copyright (C) 1999 Federation of European Biochemical Societies.

AB - The human N-acetylglucosaminyltransferase I gene was introduced in the genome of Trichoderma reesei strain VTT-D-80133. Expression was studied after induction from the cellobiohydrolase I promoter. Successful in vivo transfer of GlcNAc was demonstrated by analyzing the neutral N-glycans which were synthesized on cellobiohydrolase I. Final proof of the formation of GlcNAcMan5GlcNAc2 was obtained by NMR analysis. Copyright (C) 1999 Federation of European Biochemical Societies.

KW - Cellobiohydrolase I

KW - Fungus

KW - N-acetylglucosaminyltransferase I

KW - Trichoderma reesei

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AN - SCOPUS:0033005982

SN - 0014-5793

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ER -

In vivo synthesis of complex N-glycans by expression of human N-acetylglucosaminyltransferase I in the filamentous fungus Trichoderma reesei

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