In vivo synthesis of complex N-glycans by expression of human N-acetylglucosaminyltransferase I in the filamentous fungus Trichoderma reesei

Marleen Maras, André De Bruyn, Wouter Vervecken, Jaana Uusitalo, Merja Penttilä, Roger Busson, Piet Herdewijn, Roland Contreras

Research output: Contribution to journalArticleScientificpeer-review

32 Citations (Scopus)

Abstract

The human N-acetylglucosaminyltransferase I gene was introduced in the genome of Trichoderma reesei strain VTT-D-80133. Expression was studied after induction from the cellobiohydrolase I promoter. Successful in vivo transfer of GlcNAc was demonstrated by analyzing the neutral N-glycans which were synthesized on cellobiohydrolase I. Final proof of the formation of GlcNAcMan5GlcNAc2 was obtained by NMR analysis. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)365-370
Number of pages6
JournalFEBS Letters
Volume452
Issue number3
DOIs
Publication statusPublished - 11 Jun 1999
MoE publication typeA1 Journal article-refereed

Fingerprint

Cellulose 1,4-beta-Cellobiosidase
Trichoderma
Fungi
Polysaccharides
Genes
Nuclear magnetic resonance
Genome
alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase I

Keywords

  • Cellobiohydrolase I
  • Fungus
  • N-acetylglucosaminyltransferase I
  • Trichoderma reesei

Cite this

Maras, Marleen ; De Bruyn, André ; Vervecken, Wouter ; Uusitalo, Jaana ; Penttilä, Merja ; Busson, Roger ; Herdewijn, Piet ; Contreras, Roland. / In vivo synthesis of complex N-glycans by expression of human N-acetylglucosaminyltransferase I in the filamentous fungus Trichoderma reesei. In: FEBS Letters. 1999 ; Vol. 452, No. 3. pp. 365-370.
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In vivo synthesis of complex N-glycans by expression of human N-acetylglucosaminyltransferase I in the filamentous fungus Trichoderma reesei. / Maras, Marleen; De Bruyn, André; Vervecken, Wouter; Uusitalo, Jaana; Penttilä, Merja; Busson, Roger; Herdewijn, Piet; Contreras, Roland.

In: FEBS Letters, Vol. 452, No. 3, 11.06.1999, p. 365-370.

Research output: Contribution to journalArticleScientificpeer-review

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