In vivo synthesis of complex N-glycans by expression of human N-acetylglucosaminyltransferase I in the filamentous fungus Trichoderma reesei

Marleen Maras, André De Bruyn, Wouter Vervecken, Jaana Uusitalo, Merja Penttilä, Roger Busson, Piet Herdewijn, Roland Contreras

    Research output: Contribution to journalArticleScientificpeer-review

    33 Citations (Scopus)

    Abstract

    The human N-acetylglucosaminyltransferase I gene was introduced in the genome of Trichoderma reesei strain VTT-D-80133. Expression was studied after induction from the cellobiohydrolase I promoter. Successful in vivo transfer of GlcNAc was demonstrated by analyzing the neutral N-glycans which were synthesized on cellobiohydrolase I. Final proof of the formation of GlcNAcMan5GlcNAc2 was obtained by NMR analysis. Copyright (C) 1999 Federation of European Biochemical Societies.

    Original languageEnglish
    Pages (from-to)365-370
    Number of pages6
    JournalFEBS Letters
    Volume452
    Issue number3
    DOIs
    Publication statusPublished - 11 Jun 1999
    MoE publication typeA1 Journal article-refereed

    Keywords

    • Cellobiohydrolase I
    • Fungus
    • N-acetylglucosaminyltransferase I
    • Trichoderma reesei

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