Incorporation of laccase into surface-sized paper

P. Ihalainen (Corresponding Author), T. Hämäläinen, K. Matilainen, A. Savolainen, T. Sipiläinen-Malm, O.-V. Kaukoniemi, A. Määttänen, T. Erho, M. Smolander, J. Peltonen

Research output: Contribution to journalArticleScientificpeer-review

1 Citation (Scopus)

Abstract

The aim of this work was to study the activity and functionality of laccase incorporated into laboratory-coated surface-sized paper. The effects of the physicochemical properties of the studied sized paper grades (permeability, porosity, and wetting) on the enzyme activity were studied. The enzymatic activity of laccase was measured either by following the oxidation of the substrate (mediator) with spectrophotometry or by monitoring the reduction of oxygen by luminescence quenching. The results showed that the initial activity of laccase incorporated into the coating did not decrease during the first 4 weeks and the activity was partly preserved even after 6 months. The functionality of the enzyme in sized papers was examined using 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) as the substrate. ABTS was inkjet-printed on the sized paper, and the reaction between laccase and ABTS appeared as a color change. The most important factor affecting the color response was the enzyme activity. Other beneficial factors affecting the color response were the hygroscopic nature and wettability of the sized paper. The results showed that the fabrication of bioactive paper is possible with a relatively simple strategy, i.e., by incorporation of the biomolecules in the coating formulation (paste). No major investments would be needed to start the production of this kind of environmentally sustainable, low-cost, and lightweight paper-based bio-indicator, e.g., for authentication applications.
Original languageEnglish
Pages (from-to)365-373
JournalJournal of Coatings Technology and Research
Volume12
Issue number2
DOIs
Publication statusPublished - 2015
MoE publication typeA1 Journal article-refereed

Fingerprint

Laccase
Enzyme activity
Color
enzyme activity
Wetting
color
Coatings
Spectrophotometry
Biomolecules
Substrates
coatings
Authentication
Sulfonic Acids
Luminescence
Quenching
sulfonic acid
spectrophotometry
Enzymes
Ointments
activity (biology)

Keywords

  • bioactive paper
  • laccase
  • ABTS
  • surface sizing
  • enzyme activity

Cite this

Ihalainen, P., Hämäläinen, T., Matilainen, K., Savolainen, A., Sipiläinen-Malm, T., Kaukoniemi, O-V., ... Peltonen, J. (2015). Incorporation of laccase into surface-sized paper. Journal of Coatings Technology and Research, 12(2), 365-373. https://doi.org/10.1007/s11998-014-9640-5
Ihalainen, P. ; Hämäläinen, T. ; Matilainen, K. ; Savolainen, A. ; Sipiläinen-Malm, T. ; Kaukoniemi, O.-V. ; Määttänen, A. ; Erho, T. ; Smolander, M. ; Peltonen, J. / Incorporation of laccase into surface-sized paper. In: Journal of Coatings Technology and Research. 2015 ; Vol. 12, No. 2. pp. 365-373.
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Ihalainen, P, Hämäläinen, T, Matilainen, K, Savolainen, A, Sipiläinen-Malm, T, Kaukoniemi, O-V, Määttänen, A, Erho, T, Smolander, M & Peltonen, J 2015, 'Incorporation of laccase into surface-sized paper', Journal of Coatings Technology and Research, vol. 12, no. 2, pp. 365-373. https://doi.org/10.1007/s11998-014-9640-5

Incorporation of laccase into surface-sized paper. / Ihalainen, P. (Corresponding Author); Hämäläinen, T.; Matilainen, K.; Savolainen, A.; Sipiläinen-Malm, T.; Kaukoniemi, O.-V.; Määttänen, A.; Erho, T.; Smolander, M.; Peltonen, J.

In: Journal of Coatings Technology and Research, Vol. 12, No. 2, 2015, p. 365-373.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Incorporation of laccase into surface-sized paper

AU - Ihalainen, P.

AU - Hämäläinen, T.

AU - Matilainen, K.

AU - Savolainen, A.

AU - Sipiläinen-Malm, T.

AU - Kaukoniemi, O.-V.

AU - Määttänen, A.

AU - Erho, T.

AU - Smolander, M.

AU - Peltonen, J.

PY - 2015

Y1 - 2015

N2 - The aim of this work was to study the activity and functionality of laccase incorporated into laboratory-coated surface-sized paper. The effects of the physicochemical properties of the studied sized paper grades (permeability, porosity, and wetting) on the enzyme activity were studied. The enzymatic activity of laccase was measured either by following the oxidation of the substrate (mediator) with spectrophotometry or by monitoring the reduction of oxygen by luminescence quenching. The results showed that the initial activity of laccase incorporated into the coating did not decrease during the first 4 weeks and the activity was partly preserved even after 6 months. The functionality of the enzyme in sized papers was examined using 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) as the substrate. ABTS was inkjet-printed on the sized paper, and the reaction between laccase and ABTS appeared as a color change. The most important factor affecting the color response was the enzyme activity. Other beneficial factors affecting the color response were the hygroscopic nature and wettability of the sized paper. The results showed that the fabrication of bioactive paper is possible with a relatively simple strategy, i.e., by incorporation of the biomolecules in the coating formulation (paste). No major investments would be needed to start the production of this kind of environmentally sustainable, low-cost, and lightweight paper-based bio-indicator, e.g., for authentication applications.

AB - The aim of this work was to study the activity and functionality of laccase incorporated into laboratory-coated surface-sized paper. The effects of the physicochemical properties of the studied sized paper grades (permeability, porosity, and wetting) on the enzyme activity were studied. The enzymatic activity of laccase was measured either by following the oxidation of the substrate (mediator) with spectrophotometry or by monitoring the reduction of oxygen by luminescence quenching. The results showed that the initial activity of laccase incorporated into the coating did not decrease during the first 4 weeks and the activity was partly preserved even after 6 months. The functionality of the enzyme in sized papers was examined using 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) as the substrate. ABTS was inkjet-printed on the sized paper, and the reaction between laccase and ABTS appeared as a color change. The most important factor affecting the color response was the enzyme activity. Other beneficial factors affecting the color response were the hygroscopic nature and wettability of the sized paper. The results showed that the fabrication of bioactive paper is possible with a relatively simple strategy, i.e., by incorporation of the biomolecules in the coating formulation (paste). No major investments would be needed to start the production of this kind of environmentally sustainable, low-cost, and lightweight paper-based bio-indicator, e.g., for authentication applications.

KW - bioactive paper

KW - laccase

KW - ABTS

KW - surface sizing

KW - enzyme activity

U2 - 10.1007/s11998-014-9640-5

DO - 10.1007/s11998-014-9640-5

M3 - Article

VL - 12

SP - 365

EP - 373

JO - Journal of Coatings Technology and Research

JF - Journal of Coatings Technology and Research

SN - 1547-0091

IS - 2

ER -

Ihalainen P, Hämäläinen T, Matilainen K, Savolainen A, Sipiläinen-Malm T, Kaukoniemi O-V et al. Incorporation of laccase into surface-sized paper. Journal of Coatings Technology and Research. 2015;12(2):365-373. https://doi.org/10.1007/s11998-014-9640-5