Incorporation of lipid-tagged single-chain antibodies into lipid monolayers and the interaction with antigen

Inger Vikholm (Corresponding Author), Erika Györvary, Jouko Peltonen

Research output: Contribution to journalArticleScientificpeer-review

20 Citations (Scopus)

Abstract

Various amounts of a bacterially produced lipid-tagged single-chain antibody were incorporated into phospholipid monolayers preformed at the air−water interface. The mixed layers were transferred onto octadecyl mercaptan-treated gold films, and the binding of hapten, which was used as antigen, was determined by surface plasmon resonance. Incorporation of the single-chain antibody, transfer of the layer onto solid slides, amount of nonspecific adsorption, and thus the amount of specific binding depended on the composition of the lipid matrix. Studies by atomic force microscopy revealed that the film consisted of antibody-enriched lipid domains and that the films were not stable when stored long times in aqueous solution.
Original languageEnglish
Pages (from-to)3276-3281
Number of pages6
JournalLangmuir
Volume12
Issue number13
DOIs
Publication statusPublished - 1996
MoE publication typeA1 Journal article-refereed

Fingerprint

Single-Chain Antibodies
antigens
Antigens
antibodies
Antibodies
Lipids
lipids
Monolayers
Haptens
Phospholipids
Surface plasmon resonance
interactions
surface plasmon resonance
chutes
Sulfhydryl Compounds
thiols
Gold
Atomic force microscopy
atomic force microscopy
gold

Cite this

Vikholm, Inger ; Györvary, Erika ; Peltonen, Jouko. / Incorporation of lipid-tagged single-chain antibodies into lipid monolayers and the interaction with antigen. In: Langmuir. 1996 ; Vol. 12, No. 13. pp. 3276-3281.
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Incorporation of lipid-tagged single-chain antibodies into lipid monolayers and the interaction with antigen. / Vikholm, Inger (Corresponding Author); Györvary, Erika; Peltonen, Jouko.

In: Langmuir, Vol. 12, No. 13, 1996, p. 3276-3281.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Incorporation of lipid-tagged single-chain antibodies into lipid monolayers and the interaction with antigen

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U2 - 10.1021/la950949c

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