Growth kinetics and indole alkaloid production ofCatharanthus roseus hairy root cultures were studied in shake flasks and in a small scale fermenter. A logistic growth model commonly used for microbes described well the growth of hairy roots. Of the several parameters analyzed during the cultivation of hairy roots, a linear relationship between sucrose consumption and dry weight increase was obtained. This suggests the validity of sugar analysis as a means in monitoring the growth of hairy roots in fermenters.
Toivonen, L., Ojala, M., & Kauppinen, V. (1990). Indole alkaloid production by hairy root cultures of catharanthus roseus: Growth kinetics and fermentation. Biotechnology Letters, 12(7), 519-524. https://doi.org/10.1007/BF01086346