Inducing homozygosity in transgenic barley (Hordeum vulgare L.) by microspore culture

Anneli Ritala (Corresponding Author), S. Marttila, Annika Wilhelmson, Anna-Maria Nuutila

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    Homozygosity was induced in transgenic barley by microspore culture. Spikes of transgenic barley plants carrying microspores in the late uni-nucleate stage were cold pretreated. Teflon rod maceration and a density of 100 000 viable micropores per plate were used. The developed calli were regenerated and plantlets were treated with colchicine. The microspore culture of 16 mother plants (three transgenic lines) resulted in 927 green regenerants. Of these plants, 476 were transferred to soil, 380 were transgenic, 358 reached maturity and 350 were fertile with a normal seed-set carrying a yield of 6.9 kg. A production efficiency of 0.8 fertile transgenic doubled haploid barley plants per spike used for microspore isolation was recorded. The produced transgenic seeds were used in malting experiments.
    Original languageEnglish
    Pages (from-to)601 - 609
    Number of pages9
    JournalActa Physiologiae Plantarum
    Issue number4
    Publication statusPublished - 2005
    MoE publication typeA1 Journal article-refereed


    • barley
    • double haploid
    • endo-beta-glucanase
    • microspore culture
    • transgenic


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