Industrial potential of lipoxygenases

Ruud Heshof, Leo H. de Graaff, Juan J. Villaverde, Armando J.D. Silvestre, Thomas Haarmann, Trine K. Dalsgaard, Johanna Buchert

Research output: Contribution to journalReview ArticleScientificpeer-review

5 Citations (Scopus)

Abstract

Lipoxygenases (LOXs) are iron- or manganese-containing oxidative enzymes found in plants, animals, bacteria and fungi. LOXs catalyze the oxidation of polyunsaturated fatty acids to the corresponding highly reactive hydroperoxides. Production of hydroperoxides by LOX can be exploited in different applications such as in bleaching of colored components, modification of lipids originating from different raw materials, production of lipid derived chemicals and production of aroma compounds. Most application research has been carried out using soybean LOX, but currently the use of microbial LOXs has also been reported. Development of LOX composition with high activity by heterologous expression in suitable production hosts would enable full exploitation of the potential of LOX derived reactions in different applications. Here, we review the biological role of LOXs, their heterologous production, as well as potential use in different applications. LOXs may fulfill an important role in the design of processes that are far more environmental friendly than currently used chemical reactions. Difficulties in screening for the optimal enzymes and producing LOX enzymes in sufficient amounts prevent large-scale application so far. With this review, we summarize current knowledge of LOX enzymes and the way in which they can be produced and applied.

Original languageEnglish
Pages (from-to)665-674
Number of pages10
JournalCritical Reviews in Biotechnology
Volume36
Issue number4
DOIs
Publication statusPublished - 3 Jul 2016
MoE publication typeA2 Review article in a scientific journal

Fingerprint

Lipoxygenases
Enzymes
Hydrogen Peroxide
Lipids
Manganese
Unsaturated Fatty Acids
Soybeans

Keywords

  • Application
  • bleaching
  • hydroperoxide
  • lipoxygenase
  • oleochemistry
  • oxylipin
  • unsaturated fatty acid

Cite this

Heshof, R., de Graaff, L. H., Villaverde, J. J., Silvestre, A. J. D., Haarmann, T., Dalsgaard, T. K., & Buchert, J. (2016). Industrial potential of lipoxygenases. Critical Reviews in Biotechnology, 36(4), 665-674. https://doi.org/10.3109/07388551.2015.1004520
Heshof, Ruud ; de Graaff, Leo H. ; Villaverde, Juan J. ; Silvestre, Armando J.D. ; Haarmann, Thomas ; Dalsgaard, Trine K. ; Buchert, Johanna. / Industrial potential of lipoxygenases. In: Critical Reviews in Biotechnology. 2016 ; Vol. 36, No. 4. pp. 665-674.
@article{18bcd3561e8745cfbf35f31c5f6b2af0,
title = "Industrial potential of lipoxygenases",
abstract = "Lipoxygenases (LOXs) are iron- or manganese-containing oxidative enzymes found in plants, animals, bacteria and fungi. LOXs catalyze the oxidation of polyunsaturated fatty acids to the corresponding highly reactive hydroperoxides. Production of hydroperoxides by LOX can be exploited in different applications such as in bleaching of colored components, modification of lipids originating from different raw materials, production of lipid derived chemicals and production of aroma compounds. Most application research has been carried out using soybean LOX, but currently the use of microbial LOXs has also been reported. Development of LOX composition with high activity by heterologous expression in suitable production hosts would enable full exploitation of the potential of LOX derived reactions in different applications. Here, we review the biological role of LOXs, their heterologous production, as well as potential use in different applications. LOXs may fulfill an important role in the design of processes that are far more environmental friendly than currently used chemical reactions. Difficulties in screening for the optimal enzymes and producing LOX enzymes in sufficient amounts prevent large-scale application so far. With this review, we summarize current knowledge of LOX enzymes and the way in which they can be produced and applied.",
keywords = "Application, bleaching, hydroperoxide, lipoxygenase, oleochemistry, oxylipin, unsaturated fatty acid",
author = "Ruud Heshof and {de Graaff}, {Leo H.} and Villaverde, {Juan J.} and Silvestre, {Armando J.D.} and Thomas Haarmann and Dalsgaard, {Trine K.} and Johanna Buchert",
year = "2016",
month = "7",
day = "3",
doi = "10.3109/07388551.2015.1004520",
language = "English",
volume = "36",
pages = "665--674",
journal = "Critical Reviews in Biotechnology",
issn = "0738-8551",
publisher = "Taylor & Francis",
number = "4",

}

Heshof, R, de Graaff, LH, Villaverde, JJ, Silvestre, AJD, Haarmann, T, Dalsgaard, TK & Buchert, J 2016, 'Industrial potential of lipoxygenases', Critical Reviews in Biotechnology, vol. 36, no. 4, pp. 665-674. https://doi.org/10.3109/07388551.2015.1004520

Industrial potential of lipoxygenases. / Heshof, Ruud; de Graaff, Leo H.; Villaverde, Juan J.; Silvestre, Armando J.D.; Haarmann, Thomas; Dalsgaard, Trine K.; Buchert, Johanna.

In: Critical Reviews in Biotechnology, Vol. 36, No. 4, 03.07.2016, p. 665-674.

Research output: Contribution to journalReview ArticleScientificpeer-review

TY - JOUR

T1 - Industrial potential of lipoxygenases

AU - Heshof, Ruud

AU - de Graaff, Leo H.

AU - Villaverde, Juan J.

AU - Silvestre, Armando J.D.

AU - Haarmann, Thomas

AU - Dalsgaard, Trine K.

AU - Buchert, Johanna

PY - 2016/7/3

Y1 - 2016/7/3

N2 - Lipoxygenases (LOXs) are iron- or manganese-containing oxidative enzymes found in plants, animals, bacteria and fungi. LOXs catalyze the oxidation of polyunsaturated fatty acids to the corresponding highly reactive hydroperoxides. Production of hydroperoxides by LOX can be exploited in different applications such as in bleaching of colored components, modification of lipids originating from different raw materials, production of lipid derived chemicals and production of aroma compounds. Most application research has been carried out using soybean LOX, but currently the use of microbial LOXs has also been reported. Development of LOX composition with high activity by heterologous expression in suitable production hosts would enable full exploitation of the potential of LOX derived reactions in different applications. Here, we review the biological role of LOXs, their heterologous production, as well as potential use in different applications. LOXs may fulfill an important role in the design of processes that are far more environmental friendly than currently used chemical reactions. Difficulties in screening for the optimal enzymes and producing LOX enzymes in sufficient amounts prevent large-scale application so far. With this review, we summarize current knowledge of LOX enzymes and the way in which they can be produced and applied.

AB - Lipoxygenases (LOXs) are iron- or manganese-containing oxidative enzymes found in plants, animals, bacteria and fungi. LOXs catalyze the oxidation of polyunsaturated fatty acids to the corresponding highly reactive hydroperoxides. Production of hydroperoxides by LOX can be exploited in different applications such as in bleaching of colored components, modification of lipids originating from different raw materials, production of lipid derived chemicals and production of aroma compounds. Most application research has been carried out using soybean LOX, but currently the use of microbial LOXs has also been reported. Development of LOX composition with high activity by heterologous expression in suitable production hosts would enable full exploitation of the potential of LOX derived reactions in different applications. Here, we review the biological role of LOXs, their heterologous production, as well as potential use in different applications. LOXs may fulfill an important role in the design of processes that are far more environmental friendly than currently used chemical reactions. Difficulties in screening for the optimal enzymes and producing LOX enzymes in sufficient amounts prevent large-scale application so far. With this review, we summarize current knowledge of LOX enzymes and the way in which they can be produced and applied.

KW - Application

KW - bleaching

KW - hydroperoxide

KW - lipoxygenase

KW - oleochemistry

KW - oxylipin

KW - unsaturated fatty acid

UR - http://www.scopus.com/inward/record.url?scp=84975152200&partnerID=8YFLogxK

U2 - 10.3109/07388551.2015.1004520

DO - 10.3109/07388551.2015.1004520

M3 - Review Article

C2 - 25641326

AN - SCOPUS:84975152200

VL - 36

SP - 665

EP - 674

JO - Critical Reviews in Biotechnology

JF - Critical Reviews in Biotechnology

SN - 0738-8551

IS - 4

ER -

Heshof R, de Graaff LH, Villaverde JJ, Silvestre AJD, Haarmann T, Dalsgaard TK et al. Industrial potential of lipoxygenases. Critical Reviews in Biotechnology. 2016 Jul 3;36(4):665-674. https://doi.org/10.3109/07388551.2015.1004520