Influence of elastin-like polypeptide and hydrophobin on recombinant hemagglutinin accumulations in transgenic tobacco plants

H T Phan, B Hause, G Hause, E Arcalis, E Stoger, D Maresch, F Altmann, Jussi Joensuu, U Conrad (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

20 Citations (Scopus)

Abstract

Fusion protein strategies are useful tools to enhance expression and to support the development of purification technologies. The capacity of fusion protein strategies to enhance expression was explored in tobacco leaves and seeds. C-terminal fusion of elastin-like polypeptides (ELP) to influenza hemagglutinin under the control of either the constitutive CaMV 35S or the seed-specific USP promoter resulted in increased accumulation in both leaves and seeds compared to the unfused hemagglutinin. The addition of a hydrophobin to the C-terminal end of hemagglutinin did not significantly increase the expression level. We show here that, depending on the target protein, both hydrophobin fusion and ELPylation combined with endoplasmic reticulum (ER) targeting induced protein bodies in leaves as well as in seeds. The N-glycosylation pattern indicated that KDEL sequence-mediated retention of leaf-derived hemagglutinins and hemagglutinin-hydrophobin fusions were not completely retained in the ER. In contrast, hemagglutinin-ELP from leaves contained only the oligomannose form, suggesting complete ER retention. In seeds, ER retention seems to be nearly complete for all three constructs. An easy and scalable purification method for ELPylated proteins using membrane-based inverse transition cycling could be applied to both leaf- and seed-expressed hemagglutinins
Original languageEnglish
Article numbere99347
JournalPLoS ONE
Volume9
Issue number6
DOIs
Publication statusPublished - 2014
MoE publication typeA1 Journal article-refereed

Fingerprint

elastin
Tobacco
Elastin
Genetically Modified Plants
Hemagglutinins
hemagglutinins
polypeptides
tobacco
Seed
genetically modified organisms
Peptides
Fusion reactions
Endoplasmic Reticulum
endoplasmic reticulum
Cotyledon
seeds
leaves
Seeds
lysyl-aspartyl-glutamyl-leucine
Purification

Cite this

Phan, H T ; Hause, B ; Hause, G ; Arcalis, E ; Stoger, E ; Maresch, D ; Altmann, F ; Joensuu, Jussi ; Conrad, U. / Influence of elastin-like polypeptide and hydrophobin on recombinant hemagglutinin accumulations in transgenic tobacco plants. In: PLoS ONE. 2014 ; Vol. 9, No. 6.
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abstract = "Fusion protein strategies are useful tools to enhance expression and to support the development of purification technologies. The capacity of fusion protein strategies to enhance expression was explored in tobacco leaves and seeds. C-terminal fusion of elastin-like polypeptides (ELP) to influenza hemagglutinin under the control of either the constitutive CaMV 35S or the seed-specific USP promoter resulted in increased accumulation in both leaves and seeds compared to the unfused hemagglutinin. The addition of a hydrophobin to the C-terminal end of hemagglutinin did not significantly increase the expression level. We show here that, depending on the target protein, both hydrophobin fusion and ELPylation combined with endoplasmic reticulum (ER) targeting induced protein bodies in leaves as well as in seeds. The N-glycosylation pattern indicated that KDEL sequence-mediated retention of leaf-derived hemagglutinins and hemagglutinin-hydrophobin fusions were not completely retained in the ER. In contrast, hemagglutinin-ELP from leaves contained only the oligomannose form, suggesting complete ER retention. In seeds, ER retention seems to be nearly complete for all three constructs. An easy and scalable purification method for ELPylated proteins using membrane-based inverse transition cycling could be applied to both leaf- and seed-expressed hemagglutinins",
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Influence of elastin-like polypeptide and hydrophobin on recombinant hemagglutinin accumulations in transgenic tobacco plants. / Phan, H T; Hause, B; Hause, G; Arcalis, E; Stoger, E; Maresch, D; Altmann, F; Joensuu, Jussi; Conrad, U (Corresponding Author).

In: PLoS ONE, Vol. 9, No. 6, e99347, 2014.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Maresch, D

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AU - Joensuu, Jussi

AU - Conrad, U

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