Influence of growth temperature on the production of antibody Fab fragments in different microbes: A host comparative analysis

Martin Dragosits, Gianni Frascotti, Lise Bernard-Granger, Felícitas Vázquez, Maria Giuliani, Kristin Baumann, Escarlata Rodríguez-Carmona, Jaana Tokkanen, Ermenegilda Parrilli, Marilyn G. Wiebe, Renate Kunert, Michael Maurer, Brigitte Gasser, Michael Sauer, Paola Branduardi, Tiina Pakula, Markku Saloheimo, Merja Penttilä, Pau Ferrer, Maria Luisa TutinoAntonio Villaverde, Danilo Porro, Diethard Mattanovich

Research output: Contribution to journalArticleScientificpeer-review

29 Citations (Scopus)

Abstract

Microorganisms encounter diverse stress conditions in their native habitats but also during fermentation processes, which have an impact on industrial process performance. These environmental stresses and the physiological reactions they trigger, including changes in the protein folding/secretion machinery, are highly interrelated. Thus, the investigation of environmental factors, which influence protein expression and secretion is still of great importance. Among all the possible stresses, temperature appears particularly important for bioreactor cultivation of recombinant hosts, as reductions of growth temperature have been reported to increase recombinant protein production in various host organisms. Therefore, the impact of temperature on the secretion of proteins with therapeutic interest, exemplified by a model antibody Fab fragment, was analyzed in five different microbial protein production hosts growing under steady-state conditions in carbon-limited chemostat cultivations. Secretory expression of the heterodimeric antibody Fab fragment was successful in all five microbial host systems, namely Saccharomyces cerevisiae, Pichia pastoris, Trichoderma reesei, Escherichia coli and Pseudoalteromonas haloplanktis. In this comparative analysis we show that a reduction of cultivation temperature during growth at constant growth rate had a positive effect on Fab 3H6 production in three of four analyzed microorganisms, indicating common physiological responses, which favor recombinant protein production in prokaryotic as well as eukaryotic microbes.

Original languageEnglish
Pages (from-to)38-46
JournalBiotechnology Progress
Volume27
Issue number1
DOIs
Publication statusPublished - 1 Jan 2011
MoE publication typeA1 Journal article-refereed

Fingerprint

Immunoglobulin Fragments
Immunoglobulin Fab Fragments
Temperature
Growth
Recombinant Proteins
Pseudoalteromonas
Physiological Stress
Proteins
Trichoderma
Pichia
Protein Folding
Bioreactors
Fermentation
Ecosystem
Saccharomyces cerevisiae
Carbon
Escherichia coli
Therapeutics

Keywords

  • Chemostat
  • Escherichia coli
  • Pichia pastoris
  • Pseudoalteromonas haloplanktis
  • Recombinant protein production
  • Saccharomyces cerevisiae
  • Trichoderma reesei

Cite this

Dragosits, M., Frascotti, G., Bernard-Granger, L., Vázquez, F., Giuliani, M., Baumann, K., ... Mattanovich, D. (2011). Influence of growth temperature on the production of antibody Fab fragments in different microbes: A host comparative analysis. Biotechnology Progress, 27(1), 38-46. https://doi.org/10.1002/btpr.524
Dragosits, Martin ; Frascotti, Gianni ; Bernard-Granger, Lise ; Vázquez, Felícitas ; Giuliani, Maria ; Baumann, Kristin ; Rodríguez-Carmona, Escarlata ; Tokkanen, Jaana ; Parrilli, Ermenegilda ; Wiebe, Marilyn G. ; Kunert, Renate ; Maurer, Michael ; Gasser, Brigitte ; Sauer, Michael ; Branduardi, Paola ; Pakula, Tiina ; Saloheimo, Markku ; Penttilä, Merja ; Ferrer, Pau ; Luisa Tutino, Maria ; Villaverde, Antonio ; Porro, Danilo ; Mattanovich, Diethard. / Influence of growth temperature on the production of antibody Fab fragments in different microbes : A host comparative analysis. In: Biotechnology Progress. 2011 ; Vol. 27, No. 1. pp. 38-46.
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Dragosits, M, Frascotti, G, Bernard-Granger, L, Vázquez, F, Giuliani, M, Baumann, K, Rodríguez-Carmona, E, Tokkanen, J, Parrilli, E, Wiebe, MG, Kunert, R, Maurer, M, Gasser, B, Sauer, M, Branduardi, P, Pakula, T, Saloheimo, M, Penttilä, M, Ferrer, P, Luisa Tutino, M, Villaverde, A, Porro, D & Mattanovich, D 2011, 'Influence of growth temperature on the production of antibody Fab fragments in different microbes: A host comparative analysis', Biotechnology Progress, vol. 27, no. 1, pp. 38-46. https://doi.org/10.1002/btpr.524

Influence of growth temperature on the production of antibody Fab fragments in different microbes : A host comparative analysis. / Dragosits, Martin; Frascotti, Gianni; Bernard-Granger, Lise; Vázquez, Felícitas; Giuliani, Maria; Baumann, Kristin; Rodríguez-Carmona, Escarlata; Tokkanen, Jaana; Parrilli, Ermenegilda; Wiebe, Marilyn G.; Kunert, Renate; Maurer, Michael; Gasser, Brigitte; Sauer, Michael; Branduardi, Paola; Pakula, Tiina; Saloheimo, Markku; Penttilä, Merja; Ferrer, Pau; Luisa Tutino, Maria; Villaverde, Antonio; Porro, Danilo; Mattanovich, Diethard (Corresponding Author).

In: Biotechnology Progress, Vol. 27, No. 1, 01.01.2011, p. 38-46.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Dragosits, Martin

AU - Frascotti, Gianni

AU - Bernard-Granger, Lise

AU - Vázquez, Felícitas

AU - Giuliani, Maria

AU - Baumann, Kristin

AU - Rodríguez-Carmona, Escarlata

AU - Tokkanen, Jaana

AU - Parrilli, Ermenegilda

AU - Wiebe, Marilyn G.

AU - Kunert, Renate

AU - Maurer, Michael

AU - Gasser, Brigitte

AU - Sauer, Michael

AU - Branduardi, Paola

AU - Pakula, Tiina

AU - Saloheimo, Markku

AU - Penttilä, Merja

AU - Ferrer, Pau

AU - Luisa Tutino, Maria

AU - Villaverde, Antonio

AU - Porro, Danilo

AU - Mattanovich, Diethard

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