Influence of interaction time on the red blood cell (dis)aggregation dynamics in vitro studied by optical tweezers

Ruixue Zhu, Tatiana Avsievich, Alexey Popov, Igor Meglinski

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

Abstract

Optical tweezers (OT) is a unique Nobel Prize winning technology that has been widely used in studying cell interaction dynamics at a single-cell level with highly accurate manipulating of living cells and the ability to detect ultra-low intercellular forces. The reversible aggregation process of red blood cells (RBCs) that strongly influences the blood rheological properties thus being critical for blood microcirculation has long been research studied. However, in spite of plentiful researches dealing with RBC aggregation behavior based on an average response of a large number of cells, the detailed mechanism and the applicability of the two coexistent yet mutually opposed models to this reversible process require additional information. In this study, a two-channel optical tweezers system is utilized to reveal the influence of the cell interaction time (0-300 sec) on the RBC (dis)aggregation dynamics. The results show that for RBC enforced disaggregation in autologous plasma, the longer the two RBCs adhere to each other, the stronger the intercellular interaction is, and the lower the degree that a certain optical pulling force can separate two cells, whereas no significant effect of cell interaction time on RBC aggregation process was observed. This observation indicates that the RBC aggregation and disaggregation in autologous plasma are governed by different mechanisms and that the hysteresis effect, namely the dependence of the disaggregation force on RBC interaction history, is a significant feature that needs special consideration in researches related to the RBC disaggregation process.

Original languageEnglish
Title of host publicationNovel Biophotonics Techniques and Applications V
EditorsArjen Amelink, Seemantini K. Nadkarni
PublisherInternational Society for Optics and Photonics SPIE
ISBN (Electronic)978-1-51062-844-1
ISBN (Print)978-1-51062-843-4
DOIs
Publication statusPublished - 1 Jan 2019
MoE publication typeA4 Article in a conference publication
EventEuropean Conference on Biomedical Optics, ECBO 2019 - München, Germany
Duration: 23 Jun 201927 Jun 2019

Publication series

SeriesProgress in Biomedical Optics and Imaging
Number60
Volume20
ISSN1605-7422
SeriesProceedings of SPIE
Volume11075
ISSN0277-786X

Conference

ConferenceEuropean Conference on Biomedical Optics, ECBO 2019
CountryGermany
CityMünchen
Period23/06/1927/06/19

Keywords

  • Aggregation
  • Disaggregation
  • Interaction time
  • Optical tweezers
  • Red blood cells

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