Influence of Ozone Iniated Processing on the Toxicity of Aerosol Particles from Small Scale Wood Combustion

Erik Z. Nordin (Corresponding Author), Oskari Uski, Robin Nyström, Pasi Jalava, Axel C. Eriksson, Johan Genberg, Pontus Roldin, Christoffer Bergvall, Roger Westerholm, Jorma Jokiniemi, Joakim H. Pagels, Christoffer Boman, Maija-Riitta Hirvonen

Research output: Contribution to journalArticleScientificpeer-review

16 Citations (Scopus)

Abstract

Black carbon containing emissions from biomass combustion are being transformed in the atmosphere upon processing induced by tropospheric ozone and UV. The knowledge today is very limited on how atmospheric processing affects the toxicological properties of the emissions. The aim of this study was to investigate the influence of ozone initiated (dark) atmospheric processing on the physicochemical and toxicological properties of particulate emissions from wood combustion. Emissions from a conventional wood stove operated at two combustion conditions (nominal and hot air starved) were diluted and transferred to a chamber. Particulate matter (PM) was collected before and after ozone addition to the chamber using an impactor. Detailed chemical and physical characterization was performed on chamber air and collected PM. The collected PM was investigated toxicologically in vitro with a mouse macrophage model, endpoints included: cell cycle analysis, viability, inflammation and genotoxicity. The results suggest that changes in the organic fraction, including polycyclic aromatic hydrocarbons (PAHs) are the main driver for differences in obtained toxicological effects. Fresh hot air starved emissions containing a higher organic and PAH mass-fraction affected cell viability stronger than fresh emissions from nominal combustion. The PAH mass fractions decreased upon aging due to chemical degradation. Dark aging increased genotoxicity, reduced viability and reduced release of inflammatory markers. These differences were statistically significant for single doses and typically less pronounced. We hypothesize that the alterations in toxicity upon simulated dark aging in the atmosphere may be caused by reaction products that form when PAHs and other organic compounds react with ozone and nitrate radicals.
Original languageEnglish
Pages (from-to)282 - 289
JournalAtmospheric Environment
Volume102
DOIs
Publication statusPublished - 2015
MoE publication typeA1 Journal article-refereed

Fingerprint

combustion
ozone
aerosol
toxicity
PAH
particulate matter
viability
genotoxicity
air
atmosphere
carbon emission
black carbon
organic compound
nitrate
biomass

Keywords

  • polycyclic aromatic hydrocarbons
  • biomass combustion
  • atmospheric chemistry
  • aging of materials
  • ozone

Cite this

Nordin, E. Z., Uski, O., Nyström, R., Jalava, P., Eriksson, A. C., Genberg, J., ... Hirvonen, M-R. (2015). Influence of Ozone Iniated Processing on the Toxicity of Aerosol Particles from Small Scale Wood Combustion. Atmospheric Environment, 102, 282 - 289. https://doi.org/10.1016/j.atmosenv.2014.11.068
Nordin, Erik Z. ; Uski, Oskari ; Nyström, Robin ; Jalava, Pasi ; Eriksson, Axel C. ; Genberg, Johan ; Roldin, Pontus ; Bergvall, Christoffer ; Westerholm, Roger ; Jokiniemi, Jorma ; Pagels, Joakim H. ; Boman, Christoffer ; Hirvonen, Maija-Riitta. / Influence of Ozone Iniated Processing on the Toxicity of Aerosol Particles from Small Scale Wood Combustion. In: Atmospheric Environment. 2015 ; Vol. 102. pp. 282 - 289.
@article{2b18db0fe0d849eeb49d6751cdeff517,
title = "Influence of Ozone Iniated Processing on the Toxicity of Aerosol Particles from Small Scale Wood Combustion",
abstract = "Black carbon containing emissions from biomass combustion are being transformed in the atmosphere upon processing induced by tropospheric ozone and UV. The knowledge today is very limited on how atmospheric processing affects the toxicological properties of the emissions. The aim of this study was to investigate the influence of ozone initiated (dark) atmospheric processing on the physicochemical and toxicological properties of particulate emissions from wood combustion. Emissions from a conventional wood stove operated at two combustion conditions (nominal and hot air starved) were diluted and transferred to a chamber. Particulate matter (PM) was collected before and after ozone addition to the chamber using an impactor. Detailed chemical and physical characterization was performed on chamber air and collected PM. The collected PM was investigated toxicologically in vitro with a mouse macrophage model, endpoints included: cell cycle analysis, viability, inflammation and genotoxicity. The results suggest that changes in the organic fraction, including polycyclic aromatic hydrocarbons (PAHs) are the main driver for differences in obtained toxicological effects. Fresh hot air starved emissions containing a higher organic and PAH mass-fraction affected cell viability stronger than fresh emissions from nominal combustion. The PAH mass fractions decreased upon aging due to chemical degradation. Dark aging increased genotoxicity, reduced viability and reduced release of inflammatory markers. These differences were statistically significant for single doses and typically less pronounced. We hypothesize that the alterations in toxicity upon simulated dark aging in the atmosphere may be caused by reaction products that form when PAHs and other organic compounds react with ozone and nitrate radicals.",
keywords = "polycyclic aromatic hydrocarbons, biomass combustion, atmospheric chemistry, aging of materials, ozone",
author = "Nordin, {Erik Z.} and Oskari Uski and Robin Nystr{\"o}m and Pasi Jalava and Eriksson, {Axel C.} and Johan Genberg and Pontus Roldin and Christoffer Bergvall and Roger Westerholm and Jorma Jokiniemi and Pagels, {Joakim H.} and Christoffer Boman and Maija-Riitta Hirvonen",
year = "2015",
doi = "10.1016/j.atmosenv.2014.11.068",
language = "English",
volume = "102",
pages = "282 -- 289",
journal = "Atmospheric Environment",
issn = "1352-2310",
publisher = "Elsevier",

}

Nordin, EZ, Uski, O, Nyström, R, Jalava, P, Eriksson, AC, Genberg, J, Roldin, P, Bergvall, C, Westerholm, R, Jokiniemi, J, Pagels, JH, Boman, C & Hirvonen, M-R 2015, 'Influence of Ozone Iniated Processing on the Toxicity of Aerosol Particles from Small Scale Wood Combustion', Atmospheric Environment, vol. 102, pp. 282 - 289. https://doi.org/10.1016/j.atmosenv.2014.11.068

Influence of Ozone Iniated Processing on the Toxicity of Aerosol Particles from Small Scale Wood Combustion. / Nordin, Erik Z. (Corresponding Author); Uski, Oskari; Nyström, Robin; Jalava, Pasi; Eriksson, Axel C.; Genberg, Johan; Roldin, Pontus; Bergvall, Christoffer; Westerholm, Roger; Jokiniemi, Jorma; Pagels, Joakim H.; Boman, Christoffer; Hirvonen, Maija-Riitta.

In: Atmospheric Environment, Vol. 102, 2015, p. 282 - 289.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Influence of Ozone Iniated Processing on the Toxicity of Aerosol Particles from Small Scale Wood Combustion

AU - Nordin, Erik Z.

AU - Uski, Oskari

AU - Nyström, Robin

AU - Jalava, Pasi

AU - Eriksson, Axel C.

AU - Genberg, Johan

AU - Roldin, Pontus

AU - Bergvall, Christoffer

AU - Westerholm, Roger

AU - Jokiniemi, Jorma

AU - Pagels, Joakim H.

AU - Boman, Christoffer

AU - Hirvonen, Maija-Riitta

PY - 2015

Y1 - 2015

N2 - Black carbon containing emissions from biomass combustion are being transformed in the atmosphere upon processing induced by tropospheric ozone and UV. The knowledge today is very limited on how atmospheric processing affects the toxicological properties of the emissions. The aim of this study was to investigate the influence of ozone initiated (dark) atmospheric processing on the physicochemical and toxicological properties of particulate emissions from wood combustion. Emissions from a conventional wood stove operated at two combustion conditions (nominal and hot air starved) were diluted and transferred to a chamber. Particulate matter (PM) was collected before and after ozone addition to the chamber using an impactor. Detailed chemical and physical characterization was performed on chamber air and collected PM. The collected PM was investigated toxicologically in vitro with a mouse macrophage model, endpoints included: cell cycle analysis, viability, inflammation and genotoxicity. The results suggest that changes in the organic fraction, including polycyclic aromatic hydrocarbons (PAHs) are the main driver for differences in obtained toxicological effects. Fresh hot air starved emissions containing a higher organic and PAH mass-fraction affected cell viability stronger than fresh emissions from nominal combustion. The PAH mass fractions decreased upon aging due to chemical degradation. Dark aging increased genotoxicity, reduced viability and reduced release of inflammatory markers. These differences were statistically significant for single doses and typically less pronounced. We hypothesize that the alterations in toxicity upon simulated dark aging in the atmosphere may be caused by reaction products that form when PAHs and other organic compounds react with ozone and nitrate radicals.

AB - Black carbon containing emissions from biomass combustion are being transformed in the atmosphere upon processing induced by tropospheric ozone and UV. The knowledge today is very limited on how atmospheric processing affects the toxicological properties of the emissions. The aim of this study was to investigate the influence of ozone initiated (dark) atmospheric processing on the physicochemical and toxicological properties of particulate emissions from wood combustion. Emissions from a conventional wood stove operated at two combustion conditions (nominal and hot air starved) were diluted and transferred to a chamber. Particulate matter (PM) was collected before and after ozone addition to the chamber using an impactor. Detailed chemical and physical characterization was performed on chamber air and collected PM. The collected PM was investigated toxicologically in vitro with a mouse macrophage model, endpoints included: cell cycle analysis, viability, inflammation and genotoxicity. The results suggest that changes in the organic fraction, including polycyclic aromatic hydrocarbons (PAHs) are the main driver for differences in obtained toxicological effects. Fresh hot air starved emissions containing a higher organic and PAH mass-fraction affected cell viability stronger than fresh emissions from nominal combustion. The PAH mass fractions decreased upon aging due to chemical degradation. Dark aging increased genotoxicity, reduced viability and reduced release of inflammatory markers. These differences were statistically significant for single doses and typically less pronounced. We hypothesize that the alterations in toxicity upon simulated dark aging in the atmosphere may be caused by reaction products that form when PAHs and other organic compounds react with ozone and nitrate radicals.

KW - polycyclic aromatic hydrocarbons

KW - biomass combustion

KW - atmospheric chemistry

KW - aging of materials

KW - ozone

U2 - 10.1016/j.atmosenv.2014.11.068

DO - 10.1016/j.atmosenv.2014.11.068

M3 - Article

VL - 102

SP - 282

EP - 289

JO - Atmospheric Environment

JF - Atmospheric Environment

SN - 1352-2310

ER -