Influence of processing conditions on Bifidobacterium animalis subsp. lactis functionality with a special focus on acid tolerance and factors affecting it

Jaana Mättö (Corresponding Author), Hanna-Leena Alakomi, Anu Vaari, Ilkka Virkajärvi, Maria Saarela

Research output: Contribution to journalArticleScientificpeer-review

57 Citations (Scopus)

Abstract

Due to its technological feasibility, Bifidobacterium animalis subsp. lactis is the most widely used Bifidobacterium species in probiotic applications. Fermenter-scale growth of B. animalis subsp. lactis in GEM (a medium supporting well the growth of bifidobacteria) followed by freeze-drying produced preparations with good storage stability. However, a decrease in the acid tolerance of preparations was observed during the storage at elevated temperatures. Pepsin protected the cells during an exposure to low pH. Addition of pepstatin A (pepsin inhibitor) or N,N′-dicyclohexylcarbodiimide (DCCD; an inhibitor of proton translocating enzymes) decreased the protective ability at pH 2.0, while heat treatment did not have an influence on it. Measurement of membrane potential changes (DiBAC4(3)) showed that pepsin decreased hyperpolarization of the cells during an exposure to low pH suggesting that the protective ability is linked to the H+-ATPase activity of the cells. Our findings indicate a need for assessing parameters related to cell functionality in addition to culture-based stability of probiotic preparations.

Original languageEnglish
Pages (from-to)1029-1037
Number of pages9
JournalInternational Dairy Journal
Volume16
Issue number9
DOIs
Publication statusPublished - 2006
MoE publication typeA1 Journal article-refereed

Fingerprint

acid tolerance
Pepsin A
pepsin
Dicyclohexylcarbodiimide
Acids
Bifidobacterium
Probiotics
probiotics
genetically engineered microorganisms
cells
H-transporting ATP synthase
Proton-Translocating ATPases
Freeze Drying
fermenters
freeze drying
Growth
membrane potential
Membrane Potentials
protons
Protons

Keywords

  • Bifidobacterium animalis subsp. lactis
  • Acid tolerance
  • Pepsin
  • Freeze drying
  • Stability

Cite this

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title = "Influence of processing conditions on Bifidobacterium animalis subsp. lactis functionality with a special focus on acid tolerance and factors affecting it",
abstract = "Due to its technological feasibility, Bifidobacterium animalis subsp. lactis is the most widely used Bifidobacterium species in probiotic applications. Fermenter-scale growth of B. animalis subsp. lactis in GEM (a medium supporting well the growth of bifidobacteria) followed by freeze-drying produced preparations with good storage stability. However, a decrease in the acid tolerance of preparations was observed during the storage at elevated temperatures. Pepsin protected the cells during an exposure to low pH. Addition of pepstatin A (pepsin inhibitor) or N,N′-dicyclohexylcarbodiimide (DCCD; an inhibitor of proton translocating enzymes) decreased the protective ability at pH 2.0, while heat treatment did not have an influence on it. Measurement of membrane potential changes (DiBAC4(3)) showed that pepsin decreased hyperpolarization of the cells during an exposure to low pH suggesting that the protective ability is linked to the H+-ATPase activity of the cells. Our findings indicate a need for assessing parameters related to cell functionality in addition to culture-based stability of probiotic preparations.",
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Influence of processing conditions on Bifidobacterium animalis subsp. lactis functionality with a special focus on acid tolerance and factors affecting it. / Mättö, Jaana (Corresponding Author); Alakomi, Hanna-Leena; Vaari, Anu; Virkajärvi, Ilkka; Saarela, Maria.

In: International Dairy Journal, Vol. 16, No. 9, 2006, p. 1029-1037.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Influence of processing conditions on Bifidobacterium animalis subsp. lactis functionality with a special focus on acid tolerance and factors affecting it

AU - Mättö, Jaana

AU - Alakomi, Hanna-Leena

AU - Vaari, Anu

AU - Virkajärvi, Ilkka

AU - Saarela, Maria

PY - 2006

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AB - Due to its technological feasibility, Bifidobacterium animalis subsp. lactis is the most widely used Bifidobacterium species in probiotic applications. Fermenter-scale growth of B. animalis subsp. lactis in GEM (a medium supporting well the growth of bifidobacteria) followed by freeze-drying produced preparations with good storage stability. However, a decrease in the acid tolerance of preparations was observed during the storage at elevated temperatures. Pepsin protected the cells during an exposure to low pH. Addition of pepstatin A (pepsin inhibitor) or N,N′-dicyclohexylcarbodiimide (DCCD; an inhibitor of proton translocating enzymes) decreased the protective ability at pH 2.0, while heat treatment did not have an influence on it. Measurement of membrane potential changes (DiBAC4(3)) showed that pepsin decreased hyperpolarization of the cells during an exposure to low pH suggesting that the protective ability is linked to the H+-ATPase activity of the cells. Our findings indicate a need for assessing parameters related to cell functionality in addition to culture-based stability of probiotic preparations.

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