Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 1029-1037 |
| Number of pages | 9 |
| Journal | International Dairy Journal |
| Volume | 16 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2006 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- Bifidobacterium animalis subsp. lactis
- Acid tolerance
- Pepsin
- Freeze drying
- Stability
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Influence of processing conditions on Bifidobacterium animalis subsp. lactis functionality with a special focus on acid tolerance and factors affecting it. / Mättö, Jaana (Corresponding Author); Alakomi, Hanna-Leena; Vaari, Anu; Virkajärvi, Ilkka; Saarela, Maria.
In: International Dairy Journal, Vol. 16, No. 9, 2006, p. 1029-1037.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Influence of processing conditions on Bifidobacterium animalis subsp. lactis functionality with a special focus on acid tolerance and factors affecting it
AU - Mättö, Jaana
AU - Alakomi, Hanna-Leena
AU - Vaari, Anu
AU - Virkajärvi, Ilkka
AU - Saarela, Maria
PY - 2006
Y1 - 2006
N2 - Due to its technological feasibility, Bifidobacterium animalis subsp. lactis is the most widely used Bifidobacterium species in probiotic applications. Fermenter-scale growth of B. animalis subsp. lactis in GEM (a medium supporting well the growth of bifidobacteria) followed by freeze-drying produced preparations with good storage stability. However, a decrease in the acid tolerance of preparations was observed during the storage at elevated temperatures. Pepsin protected the cells during an exposure to low pH. Addition of pepstatin A (pepsin inhibitor) or N,N′-dicyclohexylcarbodiimide (DCCD; an inhibitor of proton translocating enzymes) decreased the protective ability at pH 2.0, while heat treatment did not have an influence on it. Measurement of membrane potential changes (DiBAC4(3)) showed that pepsin decreased hyperpolarization of the cells during an exposure to low pH suggesting that the protective ability is linked to the H+-ATPase activity of the cells. Our findings indicate a need for assessing parameters related to cell functionality in addition to culture-based stability of probiotic preparations.
AB - Due to its technological feasibility, Bifidobacterium animalis subsp. lactis is the most widely used Bifidobacterium species in probiotic applications. Fermenter-scale growth of B. animalis subsp. lactis in GEM (a medium supporting well the growth of bifidobacteria) followed by freeze-drying produced preparations with good storage stability. However, a decrease in the acid tolerance of preparations was observed during the storage at elevated temperatures. Pepsin protected the cells during an exposure to low pH. Addition of pepstatin A (pepsin inhibitor) or N,N′-dicyclohexylcarbodiimide (DCCD; an inhibitor of proton translocating enzymes) decreased the protective ability at pH 2.0, while heat treatment did not have an influence on it. Measurement of membrane potential changes (DiBAC4(3)) showed that pepsin decreased hyperpolarization of the cells during an exposure to low pH suggesting that the protective ability is linked to the H+-ATPase activity of the cells. Our findings indicate a need for assessing parameters related to cell functionality in addition to culture-based stability of probiotic preparations.
KW - Bifidobacterium animalis subsp. lactis
KW - Acid tolerance
KW - Pepsin
KW - Freeze drying
KW - Stability
U2 - 10.1016/j.idairyj.2005.10.014
DO - 10.1016/j.idairyj.2005.10.014
M3 - Article
VL - 16
SP - 1029
EP - 1037
JO - International Dairy Journal
JF - International Dairy Journal
SN - 0958-6946
IS - 9
ER -