Influence of variation in methodology on the reliability of the isoelectric focusing method of fish species identification

H. Rehbein, M. Etienne, M. Jerome, Tapani Hattula, L. Knudsen, F. Jessen, J. Luten, W. Bouquet, I. Mackie, A. Ritchie, R. Martin, R. Mendes

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Abstract

The reliability of isoelectric focusing (IEF) of sarcoplasmic proteins for fish species identification was evaluated by a collaborative study among eight European laboratories. Each laboratory used its own method of IEF to identify 10 unknown samples of raw muscle by means of reference material. In 93% of cases the assignment between sample and reference was correct.

In a second study, the influence of extradant (water, low ionic strength buffer, or detergent) and the position of sample application on the protein pattern was examined. Working with light muscle of rainbow trout (Oncorhynchus mykiss), it was found that the type of extractant did not influence the protein pattern. Comparison of the patterns of samples, which had been applied near the anode, in the middle, or near the cathode, revealed differences in the number and position of the protein bands under the experimental conditions applied by most laboratories. This effect was not observed with the Phast System.

Original languageEnglish
Pages (from-to)193-197
JournalFood Chemistry
Volume52
Issue number2
DOIs
Publication statusPublished - 1995
MoE publication typeA1 Journal article-refereed

Fingerprint

isoelectric focusing
Isoelectric Focusing
Fish
Fishes
Oncorhynchus mykiss
Muscle
Electrodes
fish
Fish Proteins
Muscles
Proteins
proteins
Ionic strength
sampling
Detergents
muscles
Osmolar Concentration
Buffers
Anodes
Cathodes

Cite this

Rehbein, H. ; Etienne, M. ; Jerome, M. ; Hattula, Tapani ; Knudsen, L. ; Jessen, F. ; Luten, J. ; Bouquet, W. ; Mackie, I. ; Ritchie, A. ; Martin, R. ; Mendes, R. / Influence of variation in methodology on the reliability of the isoelectric focusing method of fish species identification. In: Food Chemistry. 1995 ; Vol. 52, No. 2. pp. 193-197.
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abstract = "The reliability of isoelectric focusing (IEF) of sarcoplasmic proteins for fish species identification was evaluated by a collaborative study among eight European laboratories. Each laboratory used its own method of IEF to identify 10 unknown samples of raw muscle by means of reference material. In 93{\%} of cases the assignment between sample and reference was correct.In a second study, the influence of extradant (water, low ionic strength buffer, or detergent) and the position of sample application on the protein pattern was examined. Working with light muscle of rainbow trout (Oncorhynchus mykiss), it was found that the type of extractant did not influence the protein pattern. Comparison of the patterns of samples, which had been applied near the anode, in the middle, or near the cathode, revealed differences in the number and position of the protein bands under the experimental conditions applied by most laboratories. This effect was not observed with the Phast System.",
author = "H. Rehbein and M. Etienne and M. Jerome and Tapani Hattula and L. Knudsen and F. Jessen and J. Luten and W. Bouquet and I. Mackie and A. Ritchie and R. Martin and R. Mendes",
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Rehbein, H, Etienne, M, Jerome, M, Hattula, T, Knudsen, L, Jessen, F, Luten, J, Bouquet, W, Mackie, I, Ritchie, A, Martin, R & Mendes, R 1995, 'Influence of variation in methodology on the reliability of the isoelectric focusing method of fish species identification', Food Chemistry, vol. 52, no. 2, pp. 193-197. https://doi.org/0.1016/0308-8146(94)P4203-R

Influence of variation in methodology on the reliability of the isoelectric focusing method of fish species identification. / Rehbein, H.; Etienne, M.; Jerome, M.; Hattula, Tapani; Knudsen, L.; Jessen, F.; Luten, J.; Bouquet, W.; Mackie, I.; Ritchie, A.; Martin, R.; Mendes, R.

In: Food Chemistry, Vol. 52, No. 2, 1995, p. 193-197.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Influence of variation in methodology on the reliability of the isoelectric focusing method of fish species identification

AU - Rehbein, H.

AU - Etienne, M.

AU - Jerome, M.

AU - Hattula, Tapani

AU - Knudsen, L.

AU - Jessen, F.

AU - Luten, J.

AU - Bouquet, W.

AU - Mackie, I.

AU - Ritchie, A.

AU - Martin, R.

AU - Mendes, R.

N1 - Project code: BEL4273

PY - 1995

Y1 - 1995

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AB - The reliability of isoelectric focusing (IEF) of sarcoplasmic proteins for fish species identification was evaluated by a collaborative study among eight European laboratories. Each laboratory used its own method of IEF to identify 10 unknown samples of raw muscle by means of reference material. In 93% of cases the assignment between sample and reference was correct.In a second study, the influence of extradant (water, low ionic strength buffer, or detergent) and the position of sample application on the protein pattern was examined. Working with light muscle of rainbow trout (Oncorhynchus mykiss), it was found that the type of extractant did not influence the protein pattern. Comparison of the patterns of samples, which had been applied near the anode, in the middle, or near the cathode, revealed differences in the number and position of the protein bands under the experimental conditions applied by most laboratories. This effect was not observed with the Phast System.

U2 - 0.1016/0308-8146(94)P4203-R

DO - 0.1016/0308-8146(94)P4203-R

M3 - Article

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