TY - JOUR
T1 - Inhibitory effect of lignin during cellulose bioconversion
T2 - The effect of lignin chemistry on non-productive enzyme adsorption
AU - Rahikainen, Jenni
AU - Martin-Sampedro, Raquel
AU - Heikkinen, Harri
AU - Rovio, Stella
AU - Marjamaa, Kaisa
AU - Tamminen, Tarja
AU - Rojas, Orlando J.
AU - Kruus, Kristiina
PY - 2013
Y1 - 2013
N2 - The effect of lignin as an inhibitory biopolymer for the enzymatic hydrolysis of lignocellulosic biomass was studied; specially addressing the role of lignin in non-productive enzyme adsorption. Botanical origin and biomass pre-treatment give rise to differences in lignin structure and the effect of these differences on enzyme binding and inhibition were elucidated. Lignin was isolated from steam explosion (SE) pre-treated and non-treated spruce and wheat straw and used for the preparation of ultrathin films for enzyme binding studies. Binding of Trichoderma reesei Cel7A (CBHI) and the corresponding Cel7A-core, lacking the linker and the cellulose-binding domain, to the lignin films was monitored using a quartz crystal microbalance (QCM). SE pre-treatment altered the lignin structure, leading to increased enzyme adsorption. Thus, the positive effect of SE pre-treatment, opening the cell wall matrix to make polysaccharides more accessible, may be compromised by the structural changes of lignin that increase non-productive enzyme adsorption.
AB - The effect of lignin as an inhibitory biopolymer for the enzymatic hydrolysis of lignocellulosic biomass was studied; specially addressing the role of lignin in non-productive enzyme adsorption. Botanical origin and biomass pre-treatment give rise to differences in lignin structure and the effect of these differences on enzyme binding and inhibition were elucidated. Lignin was isolated from steam explosion (SE) pre-treated and non-treated spruce and wheat straw and used for the preparation of ultrathin films for enzyme binding studies. Binding of Trichoderma reesei Cel7A (CBHI) and the corresponding Cel7A-core, lacking the linker and the cellulose-binding domain, to the lignin films was monitored using a quartz crystal microbalance (QCM). SE pre-treatment altered the lignin structure, leading to increased enzyme adsorption. Thus, the positive effect of SE pre-treatment, opening the cell wall matrix to make polysaccharides more accessible, may be compromised by the structural changes of lignin that increase non-productive enzyme adsorption.
KW - carbohydrate binding module
KW - cellulase binding
KW - lignin
KW - steam explosion pre-treatment
KW - trichoderma reesei Cel7A
U2 - 10.1016/j.biortech.2013.01.075
DO - 10.1016/j.biortech.2013.01.075
M3 - Article
SN - 0960-8524
VL - 133
SP - 270
EP - 278
JO - Bioresource Technology
JF - Bioresource Technology
ER -