Inhibitory effect of lignin during cellulose bioconversion

The effect of lignin chemistry on non-productive enzyme adsorption

Jenni Rahikainen (Corresponding Author), R. Martin-Sampedro, Harri Heikkinen, Stella Rovio, Kaisa Marjamaa, Tarja Tamminen, O.J. Rojas, Kristiina Kruus

Research output: Contribution to journalArticleScientificpeer-review

193 Citations (Scopus)

Abstract

The effect of lignin as an inhibitory biopolymer for the enzymatic hydrolysis of lignocellulosic biomass was studied; specially addressing the role of lignin in non-productive enzyme adsorption. Botanical origin and biomass pre-treatment give rise to differences in lignin structure and the effect of these differences on enzyme binding and inhibition were elucidated. Lignin was isolated from steam explosion (SE) pre-treated and non-treated spruce and wheat straw and used for the preparation of ultrathin films for enzyme binding studies. Binding of Trichoderma reesei Cel7A (CBHI) and the corresponding Cel7A-core, lacking the linker and the cellulose-binding domain, to the lignin films was monitored using a quartz crystal microbalance (QCM). SE pre-treatment altered the lignin structure, leading to increased enzyme adsorption. Thus, the positive effect of SE pre-treatment, opening the cell wall matrix to make polysaccharides more accessible, may be compromised by the structural changes of lignin that increase non-productive enzyme adsorption.
Original languageEnglish
Pages (from-to)270-278
Number of pages9
JournalBioresource Technology
Volume133
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

Fingerprint

Bioconversion
Lignin
Cellulose
lignin
cellulose
Enzymes
enzyme
adsorption
Adsorption
Steam
Explosions
explosion
Biomass
Enzyme inhibition
Biopolymers
Enzymatic hydrolysis
Ultrathin films
Quartz crystal microbalances
biomass
Straw

Keywords

  • carbohydrate binding module
  • cellulase binding
  • lignin
  • steam explosion pre-treatment
  • trichoderma reesei Cel7A

Cite this

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title = "Inhibitory effect of lignin during cellulose bioconversion: The effect of lignin chemistry on non-productive enzyme adsorption",
abstract = "The effect of lignin as an inhibitory biopolymer for the enzymatic hydrolysis of lignocellulosic biomass was studied; specially addressing the role of lignin in non-productive enzyme adsorption. Botanical origin and biomass pre-treatment give rise to differences in lignin structure and the effect of these differences on enzyme binding and inhibition were elucidated. Lignin was isolated from steam explosion (SE) pre-treated and non-treated spruce and wheat straw and used for the preparation of ultrathin films for enzyme binding studies. Binding of Trichoderma reesei Cel7A (CBHI) and the corresponding Cel7A-core, lacking the linker and the cellulose-binding domain, to the lignin films was monitored using a quartz crystal microbalance (QCM). SE pre-treatment altered the lignin structure, leading to increased enzyme adsorption. Thus, the positive effect of SE pre-treatment, opening the cell wall matrix to make polysaccharides more accessible, may be compromised by the structural changes of lignin that increase non-productive enzyme adsorption.",
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Inhibitory effect of lignin during cellulose bioconversion : The effect of lignin chemistry on non-productive enzyme adsorption. / Rahikainen, Jenni (Corresponding Author); Martin-Sampedro, R.; Heikkinen, Harri; Rovio, Stella; Marjamaa, Kaisa; Tamminen, Tarja; Rojas, O.J.; Kruus, Kristiina.

In: Bioresource Technology, Vol. 133, 2013, p. 270-278.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Inhibitory effect of lignin during cellulose bioconversion

T2 - The effect of lignin chemistry on non-productive enzyme adsorption

AU - Rahikainen, Jenni

AU - Martin-Sampedro, R.

AU - Heikkinen, Harri

AU - Rovio, Stella

AU - Marjamaa, Kaisa

AU - Tamminen, Tarja

AU - Rojas, O.J.

AU - Kruus, Kristiina

PY - 2013

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AB - The effect of lignin as an inhibitory biopolymer for the enzymatic hydrolysis of lignocellulosic biomass was studied; specially addressing the role of lignin in non-productive enzyme adsorption. Botanical origin and biomass pre-treatment give rise to differences in lignin structure and the effect of these differences on enzyme binding and inhibition were elucidated. Lignin was isolated from steam explosion (SE) pre-treated and non-treated spruce and wheat straw and used for the preparation of ultrathin films for enzyme binding studies. Binding of Trichoderma reesei Cel7A (CBHI) and the corresponding Cel7A-core, lacking the linker and the cellulose-binding domain, to the lignin films was monitored using a quartz crystal microbalance (QCM). SE pre-treatment altered the lignin structure, leading to increased enzyme adsorption. Thus, the positive effect of SE pre-treatment, opening the cell wall matrix to make polysaccharides more accessible, may be compromised by the structural changes of lignin that increase non-productive enzyme adsorption.

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KW - cellulase binding

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