@article{7efbd1e3a97b448fbfb3d439b552e28a,
title = "Inkjet-printed electrochemically reduced graphene oxide microelectrode as a platform for HT-2 mycotoxin immunoenzymatic biosensing",
abstract = "The design and application of an inkjet-printed electrochemically reduced graphene oxide microelectrode for HT-2 mycotoxin immunoenzymatic biosensing is reported. A water-based graphene oxide ink was first formulated and single-drop line working microelectrodes were inkjet-printed onto poly(ethylene 2,6-naphthalate) substrates, with dimensions of 78 μm in width and 30 nm in height after solvent evaporation. The printed graphene oxide microelectrodes were electrochemically reduced and characterized by Raman and X-ray photoelectron spectroscopies in addition to microscopies. Through optimization of the electrochemical reduction parameters, differential pulse voltammetry were performed to examine the sensing of 1-naphthol (1-N), where it was revealed that reduction times had significant effects on electrode performance. The developed microelectrodes were then used as an immunoenzymatic biosensor for the detection of HT-2 mycotoxin based on carbodiimide linking of the microelectrode surface and HT-2 toxin antigen binding fragment of antibody (anti-HT2 (10) Fab). The HT-2 toxin and anti-HT2 (10) Fab reaction was reported by anti-HT2 immune complex single-chain variable fragment of antibody fused with alkaline phosphatase (anti-IC-HT2 scFv-ALP) which is able to produce an electroactive reporter – 1-N. The biosensor showed detection limit of 1.6 ng ∙ mL−1 and a linear dynamic range of 6.3 – 100.0 ng ∙ mL−1 within a 5 min incubation with 1-naphthyl phosphate (1-NP) substrate.",
keywords = "2D material, Antibody, Biosensor, Electrochemistry, Graphene oxide",
author = "Jiri Kudr and Lei Zhao and Nguyen, {Emily P.} and Henri Arola and Nevanen, {Tarja K.} and Vojtech Adam and Ondrej Zitka and Arben Merko{\c c}i",
note = "Funding Information: We acknowledge financial support from EU Graphene Flagship Core 2 Project (No. 785219). This work is also funded by the CERCA Program/Generalitat de Catalunya. ICN2 acknowledges the support of the Spanish MINECO for the Project MAT2017-87202-P and through the Severo Ochoa Centers of Excellence Program under Grant SEV2201320295. Financial support from CEITEC 2020 (LQ1601) and Grant Agency of the Czech Republic (GACR 20-30129Y) is acknowledged. The AFM measurements (GO characterization) performed at Nanobiotechnology Core Facility has been financially supported by Ministry of Education, Youth and Sports of the Czech Republic under the CIISB research infrastructure project LM2015043. The authors wish to express their thanks to Marcos Rosado Iglesias, Elena Del Corro Garc{\'i}a, Guillaume Sauthier (all Catalan Institute of Nanoscience and Nanotechnology, CSIC and The Barcelona Institute of Science and Technology, Barcelona, Spain) and Jan P{\v r}ibyl for their expert assistance with SEM, Raman spectroscopy, XPS and AFM analysis, respectively. Funding Information: We acknowledge financial support from EU Graphene Flagship Core 2 Project (No. 785219 ). This work is also funded by the CERCA Program/ Generalitat de Catalunya . ICN2 acknowledges the support of the Spanish MINECO for the Project MAT2017-87202-P and through the Severo Ochoa Centers of Excellence Program under Grant SEV2201320295 . Financial support from CEITEC 2020 ( LQ1601 ) and Grant Agency of the Czech Republic ( GACR 20-30129Y ) is acknowledged. The AFM measurements (GO characterization) performed at Nanobiotechnology Core Facility has been financially supported by Ministry of Education, Youth and Sports of the Czech Republic under the CIISB research infrastructure project LM2015043 . Publisher Copyright: {\textcopyright} 2020 Elsevier B.V.",
year = "2020",
month = may,
day = "15",
doi = "10.1016/j.bios.2020.112109",
language = "English",
volume = "156",
journal = "Biosensors & Bioelectronics",
issn = "0956-5663",
publisher = "Elsevier",
}