Inkjet-printed electrochemically reduced graphene oxide microelectrode as a platform for HT-2 mycotoxin immunoenzymatic biosensing

Jiri Kudr, Lei Zhao, Emily P. Nguyen, Henri Arola, Tarja K. Nevanen, Vojtech Adam, Ondrej Zitka, Arben Merkoçi (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

3 Citations (Scopus)

Abstract

The design and application of an inkjet-printed electrochemically reduced graphene oxide microelectrode for HT-2 mycotoxin immunoenzymatic biosensing is reported. A water-based graphene oxide ink was first formulated and single-drop line working microelectrodes were inkjet-printed onto poly(ethylene 2,6-naphthalate) substrates, with dimensions of 78 μm in width and 30 nm in height after solvent evaporation. The printed graphene oxide microelectrodes were electrochemically reduced and characterized by Raman and X-ray photoelectron spectroscopies in addition to microscopies. Through optimization of the electrochemical reduction parameters, differential pulse voltammetry were performed to examine the sensing of 1-naphthol (1-N), where it was revealed that reduction times had significant effects on electrode performance. The developed microelectrodes were then used as an immunoenzymatic biosensor for the detection of HT-2 mycotoxin based on carbodiimide linking of the microelectrode surface and HT-2 toxin antigen binding fragment of antibody (anti-HT2 (10) Fab). The HT-2 toxin and anti-HT2 (10) Fab reaction was reported by anti-HT2 immune complex single-chain variable fragment of antibody fused with alkaline phosphatase (anti-IC-HT2 scFv-ALP) which is able to produce an electroactive reporter – 1-N. The biosensor showed detection limit of 1.6 ng ∙ mL−1 and a linear dynamic range of 6.3 – 100.0 ng ∙ mL−1 within a 5 min incubation with 1-naphthyl phosphate (1-NP) substrate.

Original languageEnglish
Article number112109
JournalBiosensors and Bioelectronics
Volume156
DOIs
Publication statusPublished - 15 May 2020
MoE publication typeA1 Journal article-refereed

Keywords

  • 2D material
  • Antibody
  • Biosensor
  • Electrochemistry
  • Graphene oxide

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