Integrated multilaboratory systems biology reveals differences in protein metabolism between two reference yeast strains

Andŕ B. Canelas, Nicola Harrison, Alessandro Fazio, Jie Zhang, Juha Pekka Pitkänen, Joost Van Den Brink, Barbara M. Bakker, Lara Bogner, Jildau Bouwman, Juan I. Castrillo, Ayca Cankorur, Pramote Chumnanpuen, Pascale Daran-Lapujade, Duygu Dikicioglu, Karen Van Eunen, Jennifer C. Ewald, Joseph J. Heijnen, Betul Kirdar, Ismo Mattila, Femke I.C. MensonidesAnja Niebel, Merja Penttilä, Jack T. Pronk, Matthias Reuss, Laura Salusjärvi, Uwe Sauer, David Sherman, Martin Siemann-Herzberg, Hans Westerhoff, Johannes De Winde, Dina Petranovic, Stephen G. Oliver, Christopher T. Workman, Nicola Zamboni, Jens Nielsen

Research output: Contribution to journalArticleScientificpeer-review

72 Citations (Scopus)

Abstract

The field of systems biology is often held back by difficulties in obtaining comprehensive, high-quality, quantitative data sets. In this paper, we undertook an interlaboratory effort to generate such a data set for a very large number of cellular components in the yeast Saccharomyces cerevisiae, a widely used model organism that is also used in the production of fuels, chemicals, food ingredients and pharmaceuticals. With the current focus on biofuels and sustainability, there is much interest in harnessing this species as a general cell factory. In this study, we characterized two yeast strains, under two standard growth conditions. We ensured the high quality of the experimental data by evaluating a wide range of sampling and analytical techniques. Here we show significant differences in the maximum specific growth rate and biomass yield between the two strains. On the basis of the integrated analysis of the high-throughput data, we hypothesize that differences in phenotype are due to differences in protein metabolism.

Original languageEnglish
Article number145
JournalNature Communications
Volume1
Issue number9
DOIs
Publication statusPublished - 1 Dec 2010
MoE publication typeA1 Journal article-refereed

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