Integrated transcript and metabolite profiling of the medicinal plant Catharanthus roseus

Heiko Rischer, Alain Goossens, Matej Oresic, Dirk Inze, Kirsi-Marja Oksman-Caldentey

Research output: Contribution to journalOther journal contributionScientific

Abstract

Plant-derived secondary metabolites still constitute important resources for currently prescribed drugs and for the discovery of active principles which are effective in new indication areas. The medicinal plant Catharanthus roseus (L.) G. Don has been extensively investigated during the last decades in order to utilize cell culture systems for the biotechnological production of important anticancer compounds e.g. vinblastine and vincristine. However, rational engineering of complicated metabolic networks such as the pathways leading to terpenoid indole alkaloids (TIAs) has been greatly impeded by our poor understanding of the regulation and structural organization underlying the biosynthesis. We have applied a comprehensive profiling approach based on functional genomics, which is independent of prior sequence knowledge, to monitor jasmonate-induced changes on the transcript and metabolite profiles of cell cultures. The behaviour of most of the currently known genes and metabolites involved in TIA biosynthesis plus hundreds of unknown elements could be observed in a single experiment. The integration of the expression profiles of 417 gene tags and the accumulation profiles of 178 metabolite peaks through correlation network analysis resulted in novel gene-to-metabolite networks revealing that the different branches of TIA biosynthesis as well as various other metabolic pathways are subject to differing hormonal regulation. These networks served also to identify a select number of genes and metabolites likely to be involved in the biosynthesis of TIAs. This study sets the base for a better understanding of periwinkle secondary metabolism and increases the practical potential of metabolic engineering of this important medicinal plant.
Original languageEnglish
JournalPlanta Medica
Volume72
Issue number11
DOIs
Publication statusPublished - 2006
MoE publication typeB1 Article in a scientific magazine
Event54th Annual Congress on Medicinal Plant Research - Helsinki, Finland
Duration: 29 Aug 20062 Sep 2006

Fingerprint

Secologanin Tryptamine Alkaloids
Catharanthus
Medicinal Plants
Metabolites
Biosynthesis
Genes
Metabolic Networks and Pathways
Cell Culture Techniques
Vinca
Secondary Metabolism
Metabolic Engineering
Cell culture
Vinblastine
Vincristine
Drug Discovery
Genomics
Transcriptome
Metabolic engineering
Electric network analysis
Pharmaceutical Preparations

Cite this

@article{dcb06a8d6fca4754864bf42ca7ba4186,
title = "Integrated transcript and metabolite profiling of the medicinal plant Catharanthus roseus",
abstract = "Plant-derived secondary metabolites still constitute important resources for currently prescribed drugs and for the discovery of active principles which are effective in new indication areas. The medicinal plant Catharanthus roseus (L.) G. Don has been extensively investigated during the last decades in order to utilize cell culture systems for the biotechnological production of important anticancer compounds e.g. vinblastine and vincristine. However, rational engineering of complicated metabolic networks such as the pathways leading to terpenoid indole alkaloids (TIAs) has been greatly impeded by our poor understanding of the regulation and structural organization underlying the biosynthesis. We have applied a comprehensive profiling approach based on functional genomics, which is independent of prior sequence knowledge, to monitor jasmonate-induced changes on the transcript and metabolite profiles of cell cultures. The behaviour of most of the currently known genes and metabolites involved in TIA biosynthesis plus hundreds of unknown elements could be observed in a single experiment. The integration of the expression profiles of 417 gene tags and the accumulation profiles of 178 metabolite peaks through correlation network analysis resulted in novel gene-to-metabolite networks revealing that the different branches of TIA biosynthesis as well as various other metabolic pathways are subject to differing hormonal regulation. These networks served also to identify a select number of genes and metabolites likely to be involved in the biosynthesis of TIAs. This study sets the base for a better understanding of periwinkle secondary metabolism and increases the practical potential of metabolic engineering of this important medicinal plant.",
author = "Heiko Rischer and Alain Goossens and Matej Oresic and Dirk Inze and Kirsi-Marja Oksman-Caldentey",
note = "Only abstract published",
year = "2006",
doi = "10.1055/s-2006-949790",
language = "English",
volume = "72",
journal = "Planta Medica",
issn = "0032-0943",
publisher = "Georg Thieme Verlag",
number = "11",

}

Integrated transcript and metabolite profiling of the medicinal plant Catharanthus roseus. / Rischer, Heiko; Goossens, Alain; Oresic, Matej; Inze, Dirk; Oksman-Caldentey, Kirsi-Marja.

In: Planta Medica, Vol. 72, No. 11, 2006.

Research output: Contribution to journalOther journal contributionScientific

TY - JOUR

T1 - Integrated transcript and metabolite profiling of the medicinal plant Catharanthus roseus

AU - Rischer, Heiko

AU - Goossens, Alain

AU - Oresic, Matej

AU - Inze, Dirk

AU - Oksman-Caldentey, Kirsi-Marja

N1 - Only abstract published

PY - 2006

Y1 - 2006

N2 - Plant-derived secondary metabolites still constitute important resources for currently prescribed drugs and for the discovery of active principles which are effective in new indication areas. The medicinal plant Catharanthus roseus (L.) G. Don has been extensively investigated during the last decades in order to utilize cell culture systems for the biotechnological production of important anticancer compounds e.g. vinblastine and vincristine. However, rational engineering of complicated metabolic networks such as the pathways leading to terpenoid indole alkaloids (TIAs) has been greatly impeded by our poor understanding of the regulation and structural organization underlying the biosynthesis. We have applied a comprehensive profiling approach based on functional genomics, which is independent of prior sequence knowledge, to monitor jasmonate-induced changes on the transcript and metabolite profiles of cell cultures. The behaviour of most of the currently known genes and metabolites involved in TIA biosynthesis plus hundreds of unknown elements could be observed in a single experiment. The integration of the expression profiles of 417 gene tags and the accumulation profiles of 178 metabolite peaks through correlation network analysis resulted in novel gene-to-metabolite networks revealing that the different branches of TIA biosynthesis as well as various other metabolic pathways are subject to differing hormonal regulation. These networks served also to identify a select number of genes and metabolites likely to be involved in the biosynthesis of TIAs. This study sets the base for a better understanding of periwinkle secondary metabolism and increases the practical potential of metabolic engineering of this important medicinal plant.

AB - Plant-derived secondary metabolites still constitute important resources for currently prescribed drugs and for the discovery of active principles which are effective in new indication areas. The medicinal plant Catharanthus roseus (L.) G. Don has been extensively investigated during the last decades in order to utilize cell culture systems for the biotechnological production of important anticancer compounds e.g. vinblastine and vincristine. However, rational engineering of complicated metabolic networks such as the pathways leading to terpenoid indole alkaloids (TIAs) has been greatly impeded by our poor understanding of the regulation and structural organization underlying the biosynthesis. We have applied a comprehensive profiling approach based on functional genomics, which is independent of prior sequence knowledge, to monitor jasmonate-induced changes on the transcript and metabolite profiles of cell cultures. The behaviour of most of the currently known genes and metabolites involved in TIA biosynthesis plus hundreds of unknown elements could be observed in a single experiment. The integration of the expression profiles of 417 gene tags and the accumulation profiles of 178 metabolite peaks through correlation network analysis resulted in novel gene-to-metabolite networks revealing that the different branches of TIA biosynthesis as well as various other metabolic pathways are subject to differing hormonal regulation. These networks served also to identify a select number of genes and metabolites likely to be involved in the biosynthesis of TIAs. This study sets the base for a better understanding of periwinkle secondary metabolism and increases the practical potential of metabolic engineering of this important medicinal plant.

U2 - 10.1055/s-2006-949790

DO - 10.1055/s-2006-949790

M3 - Other journal contribution

VL - 72

JO - Planta Medica

JF - Planta Medica

SN - 0032-0943

IS - 11

ER -