Abstract
Copyright © 2015 Elsevier Ltd. All rights reserved.
Inter-laboratory evaluation of SEC-post-column calcofluor for determination of the weight-average molar mass of cereal β-glucan | Request PDF. Available from: https://www.researchgate.net/publication/273507433_Inter-laboratory_evaluation_of_SEC-post-column_calcofluor_for_determination_of_the_weight-average_molar_mass_of_cereal_b-glucan [accessed Sep 18 2018].
| Original language | English |
|---|---|
| Pages (from-to) | 254-264 |
| Journal | Carbohydrate Polymers |
| Volume | 124 |
| DOIs | |
| Publication status | Published - Feb 2015 |
| MoE publication type | Not Eligible |
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Inter-laboratory evaluation of SEC-post-column calcofluor for determination of the weight-average molar mass of cereal β-glucan. / Rieder, Anne; Knutsen, Svein Halvor ; Ulset, Ann-Sissel; Christensen, Bjorn E; Andersson, Roger ; Mikkelson, Atte; Tuomainen, Päivi ; Maina, Ndegwa Henry; Ballance, Simon (Corresponding Author).
In: Carbohydrate Polymers, Vol. 124, 02.2015, p. 254-264.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Inter-laboratory evaluation of SEC-post-column calcofluor for determination of the weight-average molar mass of cereal β-glucan
AU - Rieder, Anne
AU - Knutsen, Svein Halvor
AU - Ulset, Ann-Sissel
AU - Christensen, Bjorn E
AU - Andersson, Roger
AU - Mikkelson, Atte
AU - Tuomainen, Päivi
AU - Maina, Ndegwa Henry
AU - Ballance, Simon
PY - 2015/2
Y1 - 2015/2
N2 - Even though size exclusion chromatography (SEC) with post column addition of calcofluor (SEC-calcofluor) has been used for the determination of cereal β-glucan molar mass in foods for many years, there is a lack of systematic evaluation of the method. To address this issue a set of suitable β-glucan standards were generated by preparative SEC and their molar mass characteristics were determined by analytical multi-detection SEC (refractive index (RI), light scattering). Each standard was then analysed by SEC-calcofluor at three different labs. As a direct comparison, the analyses were repeated with a RI detector. For SEC-calcofluor accurate measurements of weight average molar mass (Mw) can be made for β-glucan populations within 10-500×10(3)g/mol. Above this molar mass threshold there is an increasing tendency for underestimation of Mw. Precipitation of some β-glucan-calcofluor complexes may have delayed their transport into the detector.Copyright © 2015 Elsevier Ltd. All rights reserved. Inter-laboratory evaluation of SEC-post-column calcofluor for determination of the weight-average molar mass of cereal β-glucan | Request PDF. Available from: https://www.researchgate.net/publication/273507433_Inter-laboratory_evaluation_of_SEC-post-column_calcofluor_for_determination_of_the_weight-average_molar_mass_of_cereal_b-glucan [accessed Sep 18 2018].
AB - Even though size exclusion chromatography (SEC) with post column addition of calcofluor (SEC-calcofluor) has been used for the determination of cereal β-glucan molar mass in foods for many years, there is a lack of systematic evaluation of the method. To address this issue a set of suitable β-glucan standards were generated by preparative SEC and their molar mass characteristics were determined by analytical multi-detection SEC (refractive index (RI), light scattering). Each standard was then analysed by SEC-calcofluor at three different labs. As a direct comparison, the analyses were repeated with a RI detector. For SEC-calcofluor accurate measurements of weight average molar mass (Mw) can be made for β-glucan populations within 10-500×10(3)g/mol. Above this molar mass threshold there is an increasing tendency for underestimation of Mw. Precipitation of some β-glucan-calcofluor complexes may have delayed their transport into the detector.Copyright © 2015 Elsevier Ltd. All rights reserved. Inter-laboratory evaluation of SEC-post-column calcofluor for determination of the weight-average molar mass of cereal β-glucan | Request PDF. Available from: https://www.researchgate.net/publication/273507433_Inter-laboratory_evaluation_of_SEC-post-column_calcofluor_for_determination_of_the_weight-average_molar_mass_of_cereal_b-glucan [accessed Sep 18 2018].
U2 - 10.1016/j.carbpol.2015.02.019
DO - 10.1016/j.carbpol.2015.02.019
M3 - Article
VL - 124
SP - 254
EP - 264
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
SN - 0144-8617
ER -