Inter-laboratory evaluation of SEC-post-column calcofluor for determination of the weight-average molar mass of cereal β-glucan

Anne Rieder, Svein Halvor Knutsen, Ann-Sissel Ulset, Bjorn E Christensen, Roger Andersson, Atte Mikkelson, Päivi Tuomainen, Ndegwa Henry Maina, Simon Ballance (Corresponding Author)

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Abstract

Even though size exclusion chromatography (SEC) with post column addition of calcofluor (SEC-calcofluor) has been used for the determination of cereal β-glucan molar mass in foods for many years, there is a lack of systematic evaluation of the method. To address this issue a set of suitable β-glucan standards were generated by preparative SEC and their molar mass characteristics were determined by analytical multi-detection SEC (refractive index (RI), light scattering). Each standard was then analysed by SEC-calcofluor at three different labs. As a direct comparison, the analyses were repeated with a RI detector. For SEC-calcofluor accurate measurements of weight average molar mass (Mw) can be made for β-glucan populations within 10-500×10(3)g/mol. Above this molar mass threshold there is an increasing tendency for underestimation of Mw. Precipitation of some β-glucan-calcofluor complexes may have delayed their transport into the detector.
Copyright © 2015 Elsevier Ltd. All rights reserved.

Inter-laboratory evaluation of SEC-post-column calcofluor for determination of the weight-average molar mass of cereal β-glucan | Request PDF. Available from: https://www.researchgate.net/publication/273507433_Inter-laboratory_evaluation_of_SEC-post-column_calcofluor_for_determination_of_the_weight-average_molar_mass_of_cereal_b-glucan [accessed Sep 18 2018].
Original languageEnglish
Pages (from-to)254-264
JournalCarbohydrate Polymers
Volume124
DOIs
Publication statusPublished - Feb 2015
MoE publication typeNot Eligible

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Glucans
Size exclusion chromatography
Molar mass
Refractive index
Detectors
Light scattering

Cite this

Rieder, Anne ; Knutsen, Svein Halvor ; Ulset, Ann-Sissel ; Christensen, Bjorn E ; Andersson, Roger ; Mikkelson, Atte ; Tuomainen, Päivi ; Maina, Ndegwa Henry ; Ballance, Simon . / Inter-laboratory evaluation of SEC-post-column calcofluor for determination of the weight-average molar mass of cereal β-glucan. In: Carbohydrate Polymers. 2015 ; Vol. 124. pp. 254-264.
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title = "Inter-laboratory evaluation of SEC-post-column calcofluor for determination of the weight-average molar mass of cereal β-glucan",
abstract = "Even though size exclusion chromatography (SEC) with post column addition of calcofluor (SEC-calcofluor) has been used for the determination of cereal β-glucan molar mass in foods for many years, there is a lack of systematic evaluation of the method. To address this issue a set of suitable β-glucan standards were generated by preparative SEC and their molar mass characteristics were determined by analytical multi-detection SEC (refractive index (RI), light scattering). Each standard was then analysed by SEC-calcofluor at three different labs. As a direct comparison, the analyses were repeated with a RI detector. For SEC-calcofluor accurate measurements of weight average molar mass (Mw) can be made for β-glucan populations within 10-500×10(3)g/mol. Above this molar mass threshold there is an increasing tendency for underestimation of Mw. Precipitation of some β-glucan-calcofluor complexes may have delayed their transport into the detector.Copyright {\circledC} 2015 Elsevier Ltd. All rights reserved. Inter-laboratory evaluation of SEC-post-column calcofluor for determination of the weight-average molar mass of cereal β-glucan | Request PDF. Available from: https://www.researchgate.net/publication/273507433_Inter-laboratory_evaluation_of_SEC-post-column_calcofluor_for_determination_of_the_weight-average_molar_mass_of_cereal_b-glucan [accessed Sep 18 2018].",
author = "Anne Rieder and Knutsen, {Svein Halvor} and Ann-Sissel Ulset and Christensen, {Bjorn E} and Roger Andersson and Atte Mikkelson and P{\"a}ivi Tuomainen and Maina, {Ndegwa Henry} and Simon Ballance",
year = "2015",
month = "2",
doi = "10.1016/j.carbpol.2015.02.019",
language = "English",
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Rieder, A, Knutsen, SH, Ulset, A-S, Christensen, BE, Andersson, R, Mikkelson, A, Tuomainen, P, Maina, NH & Ballance, S 2015, 'Inter-laboratory evaluation of SEC-post-column calcofluor for determination of the weight-average molar mass of cereal β-glucan', Carbohydrate Polymers, vol. 124, pp. 254-264. https://doi.org/10.1016/j.carbpol.2015.02.019

Inter-laboratory evaluation of SEC-post-column calcofluor for determination of the weight-average molar mass of cereal β-glucan. / Rieder, Anne; Knutsen, Svein Halvor ; Ulset, Ann-Sissel; Christensen, Bjorn E; Andersson, Roger ; Mikkelson, Atte; Tuomainen, Päivi ; Maina, Ndegwa Henry; Ballance, Simon (Corresponding Author).

In: Carbohydrate Polymers, Vol. 124, 02.2015, p. 254-264.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Inter-laboratory evaluation of SEC-post-column calcofluor for determination of the weight-average molar mass of cereal β-glucan

AU - Rieder, Anne

AU - Knutsen, Svein Halvor

AU - Ulset, Ann-Sissel

AU - Christensen, Bjorn E

AU - Andersson, Roger

AU - Mikkelson, Atte

AU - Tuomainen, Päivi

AU - Maina, Ndegwa Henry

AU - Ballance, Simon

PY - 2015/2

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N2 - Even though size exclusion chromatography (SEC) with post column addition of calcofluor (SEC-calcofluor) has been used for the determination of cereal β-glucan molar mass in foods for many years, there is a lack of systematic evaluation of the method. To address this issue a set of suitable β-glucan standards were generated by preparative SEC and their molar mass characteristics were determined by analytical multi-detection SEC (refractive index (RI), light scattering). Each standard was then analysed by SEC-calcofluor at three different labs. As a direct comparison, the analyses were repeated with a RI detector. For SEC-calcofluor accurate measurements of weight average molar mass (Mw) can be made for β-glucan populations within 10-500×10(3)g/mol. Above this molar mass threshold there is an increasing tendency for underestimation of Mw. Precipitation of some β-glucan-calcofluor complexes may have delayed their transport into the detector.Copyright © 2015 Elsevier Ltd. All rights reserved. Inter-laboratory evaluation of SEC-post-column calcofluor for determination of the weight-average molar mass of cereal β-glucan | Request PDF. Available from: https://www.researchgate.net/publication/273507433_Inter-laboratory_evaluation_of_SEC-post-column_calcofluor_for_determination_of_the_weight-average_molar_mass_of_cereal_b-glucan [accessed Sep 18 2018].

AB - Even though size exclusion chromatography (SEC) with post column addition of calcofluor (SEC-calcofluor) has been used for the determination of cereal β-glucan molar mass in foods for many years, there is a lack of systematic evaluation of the method. To address this issue a set of suitable β-glucan standards were generated by preparative SEC and their molar mass characteristics were determined by analytical multi-detection SEC (refractive index (RI), light scattering). Each standard was then analysed by SEC-calcofluor at three different labs. As a direct comparison, the analyses were repeated with a RI detector. For SEC-calcofluor accurate measurements of weight average molar mass (Mw) can be made for β-glucan populations within 10-500×10(3)g/mol. Above this molar mass threshold there is an increasing tendency for underestimation of Mw. Precipitation of some β-glucan-calcofluor complexes may have delayed their transport into the detector.Copyright © 2015 Elsevier Ltd. All rights reserved. Inter-laboratory evaluation of SEC-post-column calcofluor for determination of the weight-average molar mass of cereal β-glucan | Request PDF. Available from: https://www.researchgate.net/publication/273507433_Inter-laboratory_evaluation_of_SEC-post-column_calcofluor_for_determination_of_the_weight-average_molar_mass_of_cereal_b-glucan [accessed Sep 18 2018].

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DO - 10.1016/j.carbpol.2015.02.019

M3 - Article

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SP - 254

EP - 264

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

SN - 0144-8617

ER -