Interactions of a lignin-rich fraction from Brewer's spent grain with gut microbiota in vitro

Piritta Niemi (Corresponding Author), Anna-Marja Aura, Johanna Maukonen, A.I. Smeds, Inga Mattila, Klaus Niemelä, Tarja Tamminen, C.B. Faulds, Johanna Buchert, Kaisa Poutanen

Research output: Contribution to journalArticleScientificpeer-review

12 Citations (Scopus)

Abstract

Lignin is a constituent of plant cell walls and thus is classified as part of dietary fiber. However, little is known about the role of lignin in gastrointestinal fermentation. In this work, a lignin-rich fraction was prepared from brewer’s spent grain and subjected to an in vitro colon model to study its potential bioconversions and interactions with fecal microbiota. No suppression of microbial conversion by the fraction was observed in the colon model, as measured as short-chain fatty acid production. Furthermore, no inhibition on the growth was observed when the fraction was incubated with strains of lactobacilli and bifidobacteria. In fact, the lignin-rich fraction enabled bifidobacteria to survive longer than with glucose. Several transiently appearing phenolic compounds, very likely originating from lignin, were observed during the fermentation. This would indicate that the gut microbiota was able to partially degrade lignin and metabolize the released compounds.
Original languageEnglish
Pages (from-to)6754-6762
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume61
Issue number27
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

Fingerprint

spent grains
brewers grains
Lignin
intestinal microorganisms
lignin
Bifidobacterium
Fermentation
colon
Colon
fermentation
Bioconversion
Volatile Fatty Acids
Microbiota
Dietary Fiber
Plant Cells
Lactobacillus
short chain fatty acids
biotransformation
Cell Wall
Gastrointestinal Microbiome

Keywords

  • bifidobacteria
  • brewer's spent grain
  • colon microbiota
  • dietary fiber
  • in vitro fermentation
  • lactobacilli
  • lignan
  • lignin

Cite this

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title = "Interactions of a lignin-rich fraction from Brewer's spent grain with gut microbiota in vitro",
abstract = "Lignin is a constituent of plant cell walls and thus is classified as part of dietary fiber. However, little is known about the role of lignin in gastrointestinal fermentation. In this work, a lignin-rich fraction was prepared from brewer’s spent grain and subjected to an in vitro colon model to study its potential bioconversions and interactions with fecal microbiota. No suppression of microbial conversion by the fraction was observed in the colon model, as measured as short-chain fatty acid production. Furthermore, no inhibition on the growth was observed when the fraction was incubated with strains of lactobacilli and bifidobacteria. In fact, the lignin-rich fraction enabled bifidobacteria to survive longer than with glucose. Several transiently appearing phenolic compounds, very likely originating from lignin, were observed during the fermentation. This would indicate that the gut microbiota was able to partially degrade lignin and metabolize the released compounds.",
keywords = "bifidobacteria, brewer's spent grain, colon microbiota, dietary fiber, in vitro fermentation, lactobacilli, lignan, lignin",
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Interactions of a lignin-rich fraction from Brewer's spent grain with gut microbiota in vitro. / Niemi, Piritta (Corresponding Author); Aura, Anna-Marja; Maukonen, Johanna; Smeds, A.I.; Mattila, Inga; Niemelä, Klaus; Tamminen, Tarja; Faulds, C.B.; Buchert, Johanna; Poutanen, Kaisa.

In: Journal of Agricultural and Food Chemistry, Vol. 61, No. 27, 2013, p. 6754-6762.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Interactions of a lignin-rich fraction from Brewer's spent grain with gut microbiota in vitro

AU - Niemi, Piritta

AU - Aura, Anna-Marja

AU - Maukonen, Johanna

AU - Smeds, A.I.

AU - Mattila, Inga

AU - Niemelä, Klaus

AU - Tamminen, Tarja

AU - Faulds, C.B.

AU - Buchert, Johanna

AU - Poutanen, Kaisa

PY - 2013

Y1 - 2013

N2 - Lignin is a constituent of plant cell walls and thus is classified as part of dietary fiber. However, little is known about the role of lignin in gastrointestinal fermentation. In this work, a lignin-rich fraction was prepared from brewer’s spent grain and subjected to an in vitro colon model to study its potential bioconversions and interactions with fecal microbiota. No suppression of microbial conversion by the fraction was observed in the colon model, as measured as short-chain fatty acid production. Furthermore, no inhibition on the growth was observed when the fraction was incubated with strains of lactobacilli and bifidobacteria. In fact, the lignin-rich fraction enabled bifidobacteria to survive longer than with glucose. Several transiently appearing phenolic compounds, very likely originating from lignin, were observed during the fermentation. This would indicate that the gut microbiota was able to partially degrade lignin and metabolize the released compounds.

AB - Lignin is a constituent of plant cell walls and thus is classified as part of dietary fiber. However, little is known about the role of lignin in gastrointestinal fermentation. In this work, a lignin-rich fraction was prepared from brewer’s spent grain and subjected to an in vitro colon model to study its potential bioconversions and interactions with fecal microbiota. No suppression of microbial conversion by the fraction was observed in the colon model, as measured as short-chain fatty acid production. Furthermore, no inhibition on the growth was observed when the fraction was incubated with strains of lactobacilli and bifidobacteria. In fact, the lignin-rich fraction enabled bifidobacteria to survive longer than with glucose. Several transiently appearing phenolic compounds, very likely originating from lignin, were observed during the fermentation. This would indicate that the gut microbiota was able to partially degrade lignin and metabolize the released compounds.

KW - bifidobacteria

KW - brewer's spent grain

KW - colon microbiota

KW - dietary fiber

KW - in vitro fermentation

KW - lactobacilli

KW - lignan

KW - lignin

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