Interdomain flip-flop motion visualized in flavocytochrome cellobiose dehydrogenase using high-speed atomic force microscopy during catalysis

Hirofumi Harada, Akira Onoda, Takayuki Uchihashi, Hiroki Watanabe, Naoki Sunagawa, Masahiro Samejima, Kiyohiki Igarashi, Takashi Hayashi

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)

Abstract

Cellobiose dehydrogenase (CDH) is a dual domain flavocytochrome, which consists of a dehydrogenase (DH) domain containing a flavin adenine dinucleotide and a cytochrome (CYT) domain containing b-type heme. To directly visualize the dynamic domain motion of class-I CDH from Phanerochaete chrysosporium (PcCDH) during catalysis using high-speed atomic force microscopy, the apo-form of PcCDH was anchored to a heme-immobilized flat gold surface that can specifically fix the orientation of the CYT domain. The two domains of CDH are found to be immobile in the absence of cellobiose, whereas the addition of cellobiose triggers an interdomain flip-flop motion involving domain-domain association and dissociation. Our results indicate that dynamic motion of a dual domain enzyme during catalysis induces efficient electron transfer to an external electron acceptor.
Original languageEnglish
Pages (from-to)6561-6565
Number of pages5
JournalChemical Science
Volume8
Issue number9
DOIs
Publication statusPublished - 1 Sep 2017
MoE publication typeA1 Journal article-refereed

Fingerprint

cellobiose-quinone oxidoreductase
Flip flop circuits
Catalysis
Cellobiose
Atomic force microscopy
Cytochromes
Heme
Flavin-Adenine Dinucleotide
Electrons
Gold
Oxidoreductases
Association reactions
Enzymes

Cite this

Harada, Hirofumi ; Onoda, Akira ; Uchihashi, Takayuki ; Watanabe, Hiroki ; Sunagawa, Naoki ; Samejima, Masahiro ; Igarashi, Kiyohiki ; Hayashi, Takashi. / Interdomain flip-flop motion visualized in flavocytochrome cellobiose dehydrogenase using high-speed atomic force microscopy during catalysis. In: Chemical Science. 2017 ; Vol. 8, No. 9. pp. 6561-6565.
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abstract = "Cellobiose dehydrogenase (CDH) is a dual domain flavocytochrome, which consists of a dehydrogenase (DH) domain containing a flavin adenine dinucleotide and a cytochrome (CYT) domain containing b-type heme. To directly visualize the dynamic domain motion of class-I CDH from Phanerochaete chrysosporium (PcCDH) during catalysis using high-speed atomic force microscopy, the apo-form of PcCDH was anchored to a heme-immobilized flat gold surface that can specifically fix the orientation of the CYT domain. The two domains of CDH are found to be immobile in the absence of cellobiose, whereas the addition of cellobiose triggers an interdomain flip-flop motion involving domain-domain association and dissociation. Our results indicate that dynamic motion of a dual domain enzyme during catalysis induces efficient electron transfer to an external electron acceptor.",
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Interdomain flip-flop motion visualized in flavocytochrome cellobiose dehydrogenase using high-speed atomic force microscopy during catalysis. / Harada, Hirofumi; Onoda, Akira; Uchihashi, Takayuki; Watanabe, Hiroki; Sunagawa, Naoki; Samejima, Masahiro; Igarashi, Kiyohiki; Hayashi, Takashi.

In: Chemical Science, Vol. 8, No. 9, 01.09.2017, p. 6561-6565.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Interdomain flip-flop motion visualized in flavocytochrome cellobiose dehydrogenase using high-speed atomic force microscopy during catalysis

AU - Harada, Hirofumi

AU - Onoda, Akira

AU - Uchihashi, Takayuki

AU - Watanabe, Hiroki

AU - Sunagawa, Naoki

AU - Samejima, Masahiro

AU - Igarashi, Kiyohiki

AU - Hayashi, Takashi

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