Interlaboratory coverage test on plant food bioactive compounds and their metabolites by mass spectrometry-based untargeted metabolomics

Ville Mikael Koistinen (Corresponding Author), Andreia Bento da Silva, László Abrankó, Dorrain Low, Rocio Garcia Villalba, Francisco Tomás Barberán, Rikard Landberg, Otto Savolainen, Inmaculada Alvarez-Acero, Sonia de Pascual-Teresa, Christof Van Poucke, Conceição Almeida, Lucie Petrásková, Kateřina Valentová, Stephanie Durand, Wiesław Wiczkowski, Dorota Szawara-Nowak, Raúl González-Domínguez, Rafael Llorach, Cristina Andrés-LacuevaAnna Marja Aura, Tuulikki Seppänen-Laakso, Kati Hanhineva, Claudine Manach, Maria Rosário Bronze

    Research output: Contribution to journalArticleScientificpeer-review

    18 Citations (Scopus)


    Bioactive compounds present in plant-based foods, and their metabolites derived from gut microbiota and endogenous metabolism, represent thousands of chemical structures of potential interest for human nutrition and health. State-of-the-art analytical methodologies, including untargeted metabolomics based on high-resolution mass spectrometry, are required for the profiling of these compounds in complex matrices, including plant food materials and biofluids. The aim of this project was to compare the analytical coverage of untargeted metabolomics methods independently developed and employed in various European platforms. In total, 56 chemical standards representing the most common classes of bioactive compounds spread over a wide chemical space were selected and analyzed by the participating platforms (n = 13) using their preferred untargeted method. The results were used to define analytical criteria for a successful analysis of plant food bioactives. Furthermore, they will serve as a basis for an optimized consensus method.

    Original languageEnglish
    Article number46
    Issue number3
    Publication statusPublished - 1 Sept 2018
    MoE publication typeNot Eligible


    • Mass spectrometry
    • Method development
    • Phytochemicals


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