Interpretation of interference signals in label free integrated interferometric biosensors

Hanna Heikkinen, Meng Wang, Matti Okkonen, Jukka Hast, Risto Myllylä

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review


In the future fast, simple and reliable biosensors will be needed to detect various analytes from different biosamples. This is due to fact that the needs of traditional health care are changing. In the future homecare of patients and peoples' responsibility for their own health will increase. Also, different wellness applications need new parameters to be analysed, reducing costs of traditional health care, which are increasing rapidly. One fascinating and promising sensor type for these applications is an integrated optical interferometric immunosensor, which is manufactured using organic materials. The use of organic materials opens up enormous possibilities to develop different biochemical functions. In label free biosensors the measurement is based on detecting changes in refractive index, which typically are in the range of 10-6-10-8 [1]. In this research, theoretically generated interferograms are used to compare various signal processing methods. The goal is to develop an efficient method to analyse the interferogram. Different time domain signal processing methods are studied to determine the measuring resolution and efficiency of these methods. A low cost CCD -element is used in detecting the interferogram dynamics. It was found that in most of the signal processing methods the measuring resolution was mainly limited by pixel size. With calculation of Pearson's correlation coefficient, subpixel resolution was achieved which means that nanometer range optical path differences can be measured. This results in the refractive index resolution of the order of 10-7.
Original languageEnglish
Title of host publicationOptical Diagnostics and Sensing VI
EditorsGerard L. Coté, Alexander V. Priezzhev
PublisherInternational Society for Optics and Photonics SPIE
ISBN (Print)978-0-8194-6136-0
Publication statusPublished - 2006
MoE publication typeA4 Article in a conference publication
EventOptical Diagnostics and Sensing VI - San Jose, United States
Duration: 24 Jan 200625 Jan 2006

Publication series

SeriesProceedings of SPIE


ConferenceOptical Diagnostics and Sensing VI
Country/TerritoryUnited States
CitySan Jose


  • interferometry
  • fringe pattern analysis
  • time domain methods
  • biosensor


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