Intra-individual diversity and similarity of salivary and faecal microbiota

Johanna Maukonen (Corresponding Author), Jaana Mättö, Maija-Liisa Suihko, Maria Saarela

Research output: Contribution to journalArticleScientificpeer-review

55 Citations (Scopus)

Abstract

In the present study, polyphasic analysis [cultivation, combined with the fingerprinting of individual isolates, and denaturing gradient gel electrophoresis (DGGE)] was applied to study whether similar features concerning the diversity and temporal stability of selected bacterial groups could be detected intra-individually in two different niches – the oral cavity and the colon – from ten adult volunteers consuming probiotics. The predominant bacterial microbiota, Clostridium coccoides–Eubacterium rectale group and bifidobacterial populations, were generally stable in salivary and faecal samples, with the greater diversity seen in faeces. Furthermore, different species predominated at the two different sites. Lactobacillus group DGGE profiles were unstable, yet the intra-individual profiles from faecal and salivary samples collected at the same time resembled each other. The ingested probiotic product did not affect the stability of the bacterial groups studied. The culture-based analysis showed that most subjects harboured identical indigenous Lactobacillus genotypes in saliva and faeces (Lactobacillus rhamnosus, Lactobacillus gasseri, Lactobacillus paracasei and Lactobacillus plantarum group). Thus, identical indigenous lactobacilli were able to inhabit both ends of the orogastrointestinal tract, whereas the composition of the other bacterial groups studied varied between the two sites.
Original languageEnglish
Pages (from-to)1560-1568
Number of pages9
JournalJournal of Medical Microbiology
Volume57
DOIs
Publication statusPublished - 2008
MoE publication typeA1 Journal article-refereed

Fingerprint

Microbiota
Lactobacillus
Denaturing Gradient Gel Electrophoresis
Probiotics
Feces
Lactobacillus rhamnosus
Lactobacillus plantarum
Clostridium
Saliva
Population Groups
Mouth
Volunteers
Colon
Genotype

Cite this

Maukonen, Johanna ; Mättö, Jaana ; Suihko, Maija-Liisa ; Saarela, Maria. / Intra-individual diversity and similarity of salivary and faecal microbiota. In: Journal of Medical Microbiology. 2008 ; Vol. 57. pp. 1560-1568.
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Intra-individual diversity and similarity of salivary and faecal microbiota. / Maukonen, Johanna (Corresponding Author); Mättö, Jaana; Suihko, Maija-Liisa; Saarela, Maria.

In: Journal of Medical Microbiology, Vol. 57, 2008, p. 1560-1568.

Research output: Contribution to journalArticleScientificpeer-review

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