Intravenous delivery of hydrophobin-functionalized porous silicon nanoparticles: Stability, plasma protein adsorption and biodistribution

Mirkka Sarparanta (Corresponding Author), Luis M. Bimbo, Jussi Rytkönen, Ermei Mäkilä, Timo J. Laaksonen, Päivi Laaksonen, Markus Nyman, Jarno Salonen, Markus B. Linder, Jouni Hirvonen, Hélder A. Saltos, Anu J. Airaksinen

Research output: Contribution to journalArticleScientificpeer-review

113 Citations (Scopus)

Abstract

Rapid immune recognition and subsequent elimination from the circulation hampers the use of many nanomaterials as carriers to targeted drug delivery and controlled release in the intravenous route. Here, we report the effect of a functional self-assembled protein coating on the intravenous biodistribution of 18F-labeled thermally hydrocarbonized porous silicon (THCPSi) nanoparticles in rats. 18F-Radiolabeling enables the sensitive and easy quantification of nanoparticles in tissues using radiometric methods and allows imaging of the nanoparticle biodistribution with positron emission tomography. Coating with Trichoderma reesei HFBII altered the hydrophobicity of 18F-THCPSi nanoparticles and resulted in a pronounced change in the degree of plasma protein adsorption to the nanoparticle surface in vitro. The HFBII-THCPSi nanoparticles were biocompatible in RAW 264.7 macrophages and HepG2 liver cells making their intravenous administration feasible. In vivo, the distribution of the nanoparticles between the liver and spleen, the major mononuclear phagocyte system organs in the body, was altered compared to that of uncoated 18F-THCPSi. Identification of the adsorbed proteins revealed that certain opsonins and apolipoproteins are enriched in HFBII-functionalized nanoparticles, whereas the adsorption of abundant plasma components such as serum albumin and fibrinogen is decreased.
Original languageEnglish
Pages (from-to)654-663
JournalMolecular Pharmaceutics
Volume9
Issue number3
DOIs
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed

Keywords

  • porous silicon
  • hydrophobin
  • nanoparticle
  • protein adsorption
  • biodistribution

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