Abstract
Rapid immune recognition and subsequent elimination from the circulation
hampers the use of many nanomaterials as carriers to targeted drug
delivery and controlled release in the intravenous route. Here, we
report the effect of a functional self-assembled protein coating on the
intravenous biodistribution of 18F-labeled thermally hydrocarbonized porous silicon (THCPSi) nanoparticles in rats. 18F-Radiolabeling
enables the sensitive and easy quantification of nanoparticles in
tissues using radiometric methods and allows imaging of the nanoparticle
biodistribution with positron emission tomography. Coating with Trichoderma reesei HFBII altered the hydrophobicity of 18F-THCPSi
nanoparticles and resulted in a pronounced change in the degree of
plasma protein adsorption to the nanoparticle surface in vitro.
The HFBII-THCPSi nanoparticles were biocompatible in RAW 264.7
macrophages and HepG2 liver cells making their intravenous
administration feasible. In vivo, the distribution of the
nanoparticles between the liver and spleen, the major mononuclear
phagocyte system organs in the body, was altered compared to that of
uncoated 18F-THCPSi. Identification of the adsorbed proteins
revealed that certain opsonins and apolipoproteins are enriched in
HFBII-functionalized nanoparticles, whereas the adsorption of abundant
plasma components such as serum albumin and fibrinogen is decreased.
Original language | English |
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Pages (from-to) | 654-663 |
Journal | Molecular Pharmaceutics |
Volume | 9 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2012 |
MoE publication type | A1 Journal article-refereed |
Keywords
- porous silicon
- hydrophobin
- nanoparticle
- protein adsorption
- biodistribution
- drug delivery systems
- theranostic nanomedicine