TY - CHAP
T1 - Investigation of the pentose phosphate pathway in Trichoderma reesei: disruption of the phosphoglucose isomerase gene
AU - Limon, Carmen
AU - Uusitalo, Jaana
AU - Pakula, Tiina
AU - Saloheimo, Markku
AU - Penttilä, Merja
PY - 2003
Y1 - 2003
N2 - T. reesei is widely used for industrial protein production, very little
is however known about its physiology and primary metabolism. In order to
study the role and strength of the T. reesei pentose phosphate pathway (PPP),
we have generated a phosphoglucose isomerase (PGII) disruptant in the strain
RutC30. The disruption of this gene blocks glycolysis at the second reaction
step from glucose-6-P to fructose-6-P and directs the major carbon flux to
PPP. The gene disruptants show a clearly different phenotype from the parental
strain. They do not grow with fructose, glycerol or xylose as the sole carbon
source but growth is restored if glucose is added to the media. This
indicates that glucose is needed in the cells for e.g. lipid and cell wall
component synthesis. When glucose is the only carbon source, the disruptants
display small colonies on plates and pellets in liquid media but they are able
to grow at different concentrations of glucose. Moreover, the disruptants
have an altered morphology. Glycolytic enzymes such as pyruvate kinase had
lower activity in the pgi1 disruptants than in the parental strain. On the
other hand, glucose-6P-dehydrogenase that directs glucose to PPP had somewhat
higher activity in the disruptants. The results of this study indicate that T.
reesei has a relatively active pentose phosphate pathway and in this respect
it resembles more Kluyveromyces lactis than S. cerevisiae.
AB - T. reesei is widely used for industrial protein production, very little
is however known about its physiology and primary metabolism. In order to
study the role and strength of the T. reesei pentose phosphate pathway (PPP),
we have generated a phosphoglucose isomerase (PGII) disruptant in the strain
RutC30. The disruption of this gene blocks glycolysis at the second reaction
step from glucose-6-P to fructose-6-P and directs the major carbon flux to
PPP. The gene disruptants show a clearly different phenotype from the parental
strain. They do not grow with fructose, glycerol or xylose as the sole carbon
source but growth is restored if glucose is added to the media. This
indicates that glucose is needed in the cells for e.g. lipid and cell wall
component synthesis. When glucose is the only carbon source, the disruptants
display small colonies on plates and pellets in liquid media but they are able
to grow at different concentrations of glucose. Moreover, the disruptants
have an altered morphology. Glycolytic enzymes such as pyruvate kinase had
lower activity in the pgi1 disruptants than in the parental strain. On the
other hand, glucose-6P-dehydrogenase that directs glucose to PPP had somewhat
higher activity in the disruptants. The results of this study indicate that T.
reesei has a relatively active pentose phosphate pathway and in this respect
it resembles more Kluyveromyces lactis than S. cerevisiae.
UR - https://newprairiepress.org/fgr/vol50/iss1/18/
M3 - Conference abstract in proceedings
T3 - Fungal Genetics Reports
BT - 22nd Fungal Genetics Conference at Asilomar
T2 - 22nd Fungal Genetics Conference
Y2 - 18 March 2003 through 22 March 2003
ER -