Investigation of the pentose phosphate pathway in Trichoderma reesei: disruption of the phosphoglucose isomerase gene

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

T. reesei is widely used for industrial protein production, very little is however known about its physiology and primary metabolism. In order to study the role and strength of the T. reesei pentose phosphate pathway (PPP), we have generated a phosphoglucose isomerase (PGII) disruptant in the strain RutC30. The disruption of this gene blocks glycolysis at the second reaction step from glucose-6-P to fructose-6-P and directs the major carbon flux to PPP. The gene disruptants show a clearly different phenotype from the parental strain. They do not grow with fructose, glycerol or xylose as the sole carbon source but growth is restored if glucose is added to the media. This indicates that glucose is needed in the cells for e.g. lipid and cell wall component synthesis. When glucose is the only carbon source, the disruptants display small colonies on plates and pellets in liquid media but they are able to grow at different concentrations of glucose. Moreover, the disruptants have an altered morphology. Glycolytic enzymes such as pyruvate kinase had lower activity in the pgi1 disruptants than in the parental strain. On the other hand, glucose-6P-dehydrogenase that directs glucose to PPP had somewhat higher activity in the disruptants. The results of this study indicate that T. reesei has a relatively active pentose phosphate pathway and in this respect it resembles more Kluyveromyces lactis than S. cerevisiae.
Original languageEnglish
Title of host publication22nd Fungal Genetics Conference at Asilomar
Subtitle of host publicationAbstracts from the 2003 Fungal Genetics Conference at Asilomar
DOIs
Publication statusPublished - 2003
Event22nd Fungal Genetics Conference - Asilomar, United States
Duration: 18 Mar 200322 Mar 2003

Publication series

SeriesFungal Genetics Reports
NumberArticle 18
Volume50

Conference

Conference22nd Fungal Genetics Conference
CountryUnited States
CityAsilomar
Period18/03/0322/03/03

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