Investigation of Variations in the Human Urine Metabolome amongst European Populations: An Exploratory Search for Biomarkers of People at Risk-of-Poverty

Alessia Trimigno, Bekzod Khakimov, Francesco Savorani, Leonardo Tenori, Vaiva Hendrixson, Alminas Čivilis, Marija Glibetic, Mirjana Gurinovic, Saara Pentikäinen, Janne Sallinen, Sara Garduno Diaz, Francesca Pasqui, Santosh Khokhar, Claudio Luchinat, Alessandra Bordoni, Francesco Capozzi, Søren Balling Engelsen

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Scope: According to Eurostat 2016, approximately 119 million European citizens live at-risk-of-poverty (ROP). This subpopulation is highly diverse by ethnicity, age, and culture in the different EU states, but they all have in common a low income that could represent an increased risk of nutrient deficiencies due to poor nutritional habits. This study aims to investigate the human urine metabolome in the search of common biomarkers representing dietary deficiencies amongst European populations at ROP. Methods and results: 2732 urine samples were collected from 1391 subjects across five different European countries, including the United Kingdom, Finland, Italy, Lithuania, and Serbia, and analyzed using 1H-NMR spectroscopy. The resulting urine metabolome data were explored according to study design factors including economic status, country, and gender. Conclusion: Partitioning of the effects derived from the study design factors using ANOVA-simultaneous component analysis (ASCA) revealed that country and gender effects were responsible for most of the systematic variation. The effect of economic status was, as expected, much weaker than country and gender, but more pronounced in Lithuania than in other countries. Citrate and hippurate were among the most powerful ROP biomarkers. The possible relationship between these markers and nutritional deficiencies amongst the ROP population is discussed.

Original languageEnglish
Article number1800216
JournalMolecular Nutrition and Food Research
Volume63
Issue number1
Early online date14 May 2018
DOIs
Publication statusPublished - Jan 2019
MoE publication typeNot Eligible

Fingerprint

metabolome
Metabolome
Poverty
poverty
biomarkers
urine
Biomarkers
Urine
Lithuania
socioeconomic status
nutrient deficiencies
Population
gender
experimental design
Economics
Serbia
at-risk population
Finland
nationalities and ethnic groups
Citric Acid

Keywords

  • metabolomics
  • NMR
  • nutrition
  • risk-of-poverty
  • urinary metabolome

Cite this

Trimigno, Alessia ; Khakimov, Bekzod ; Savorani, Francesco ; Tenori, Leonardo ; Hendrixson, Vaiva ; Čivilis, Alminas ; Glibetic, Marija ; Gurinovic, Mirjana ; Pentikäinen, Saara ; Sallinen, Janne ; Garduno Diaz, Sara ; Pasqui, Francesca ; Khokhar, Santosh ; Luchinat, Claudio ; Bordoni, Alessandra ; Capozzi, Francesco ; Balling Engelsen, Søren. / Investigation of Variations in the Human Urine Metabolome amongst European Populations : An Exploratory Search for Biomarkers of People at Risk-of-Poverty. In: Molecular Nutrition and Food Research. 2019 ; Vol. 63, No. 1.
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abstract = "Scope: According to Eurostat 2016, approximately 119 million European citizens live at-risk-of-poverty (ROP). This subpopulation is highly diverse by ethnicity, age, and culture in the different EU states, but they all have in common a low income that could represent an increased risk of nutrient deficiencies due to poor nutritional habits. This study aims to investigate the human urine metabolome in the search of common biomarkers representing dietary deficiencies amongst European populations at ROP. Methods and results: 2732 urine samples were collected from 1391 subjects across five different European countries, including the United Kingdom, Finland, Italy, Lithuania, and Serbia, and analyzed using 1H-NMR spectroscopy. The resulting urine metabolome data were explored according to study design factors including economic status, country, and gender. Conclusion: Partitioning of the effects derived from the study design factors using ANOVA-simultaneous component analysis (ASCA) revealed that country and gender effects were responsible for most of the systematic variation. The effect of economic status was, as expected, much weaker than country and gender, but more pronounced in Lithuania than in other countries. Citrate and hippurate were among the most powerful ROP biomarkers. The possible relationship between these markers and nutritional deficiencies amongst the ROP population is discussed.",
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Trimigno, A, Khakimov, B, Savorani, F, Tenori, L, Hendrixson, V, Čivilis, A, Glibetic, M, Gurinovic, M, Pentikäinen, S, Sallinen, J, Garduno Diaz, S, Pasqui, F, Khokhar, S, Luchinat, C, Bordoni, A, Capozzi, F & Balling Engelsen, S 2019, 'Investigation of Variations in the Human Urine Metabolome amongst European Populations: An Exploratory Search for Biomarkers of People at Risk-of-Poverty', Molecular Nutrition and Food Research, vol. 63, no. 1, 1800216. https://doi.org/10.1002/mnfr.201800216

Investigation of Variations in the Human Urine Metabolome amongst European Populations : An Exploratory Search for Biomarkers of People at Risk-of-Poverty. / Trimigno, Alessia; Khakimov, Bekzod; Savorani, Francesco; Tenori, Leonardo; Hendrixson, Vaiva; Čivilis, Alminas; Glibetic, Marija; Gurinovic, Mirjana; Pentikäinen, Saara; Sallinen, Janne; Garduno Diaz, Sara; Pasqui, Francesca; Khokhar, Santosh; Luchinat, Claudio; Bordoni, Alessandra; Capozzi, Francesco; Balling Engelsen, Søren.

In: Molecular Nutrition and Food Research, Vol. 63, No. 1, 1800216, 01.2019.

Research output: Contribution to journalArticleScientificpeer-review

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T2 - An Exploratory Search for Biomarkers of People at Risk-of-Poverty

AU - Trimigno, Alessia

AU - Khakimov, Bekzod

AU - Savorani, Francesco

AU - Tenori, Leonardo

AU - Hendrixson, Vaiva

AU - Čivilis, Alminas

AU - Glibetic, Marija

AU - Gurinovic, Mirjana

AU - Pentikäinen, Saara

AU - Sallinen, Janne

AU - Garduno Diaz, Sara

AU - Pasqui, Francesca

AU - Khokhar, Santosh

AU - Luchinat, Claudio

AU - Bordoni, Alessandra

AU - Capozzi, Francesco

AU - Balling Engelsen, Søren

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N2 - Scope: According to Eurostat 2016, approximately 119 million European citizens live at-risk-of-poverty (ROP). This subpopulation is highly diverse by ethnicity, age, and culture in the different EU states, but they all have in common a low income that could represent an increased risk of nutrient deficiencies due to poor nutritional habits. This study aims to investigate the human urine metabolome in the search of common biomarkers representing dietary deficiencies amongst European populations at ROP. Methods and results: 2732 urine samples were collected from 1391 subjects across five different European countries, including the United Kingdom, Finland, Italy, Lithuania, and Serbia, and analyzed using 1H-NMR spectroscopy. The resulting urine metabolome data were explored according to study design factors including economic status, country, and gender. Conclusion: Partitioning of the effects derived from the study design factors using ANOVA-simultaneous component analysis (ASCA) revealed that country and gender effects were responsible for most of the systematic variation. The effect of economic status was, as expected, much weaker than country and gender, but more pronounced in Lithuania than in other countries. Citrate and hippurate were among the most powerful ROP biomarkers. The possible relationship between these markers and nutritional deficiencies amongst the ROP population is discussed.

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