Investigations on the laccase-catalyzed polymerization of lignin model compounds using size-exclusion HPLC

K. Rittstieg (Corresponding Author), Anna Suurnäkki, Tapani Suortti, Kristiina Kruus, G. Guebitz, Johanna Buchert

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

The reaction of laccase from Trametes hirsuta with monomeric and dimeric lignin model compounds was studied with special attention to the formation of polymeric reaction products. Different HPLC techniques were evaluated for the analysis of the reaction products. Methods requiring a solvent change from aqueous to organic phase were found not to be adequate for the analysis of product mixtures since evaporation over nitrogen led to the polymerization of reactive monomers and extraction with dichloromethane was not quantitative in all cases. Therefore, aqueous phase size-exclusion chromatography (SEC) at high pH was used to characterize reaction product mixtures. The product molecular weight distribution (MWD) after reaction with laccase was observed to be highly dependent on the type of model compound whereas the enzyme concentration had only a limited impact.
Original languageEnglish
Pages (from-to)403-410
JournalEnzyme and Microbial Technology
Volume31
Issue number4
DOIs
Publication statusPublished - 2002
MoE publication typeA1 Journal article-refereed

Fingerprint

Laccase
Lignin
Reaction products
Polymerization
High Pressure Liquid Chromatography
Trametes
Methylene Chloride
Gel Chromatography
Nitrogen
Size exclusion chromatography
Molecular Weight
Dichloromethane
Molecular weight distribution
Evaporation
Enzymes
Monomers

Keywords

  • Laccase
  • Lignin model compounds
  • Lignin
  • Size-exclusion chromatography
  • Polymerization

Cite this

Rittstieg, K. ; Suurnäkki, Anna ; Suortti, Tapani ; Kruus, Kristiina ; Guebitz, G. ; Buchert, Johanna. / Investigations on the laccase-catalyzed polymerization of lignin model compounds using size-exclusion HPLC. In: Enzyme and Microbial Technology. 2002 ; Vol. 31, No. 4. pp. 403-410.
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Investigations on the laccase-catalyzed polymerization of lignin model compounds using size-exclusion HPLC. / Rittstieg, K. (Corresponding Author); Suurnäkki, Anna; Suortti, Tapani; Kruus, Kristiina; Guebitz, G.; Buchert, Johanna.

In: Enzyme and Microbial Technology, Vol. 31, No. 4, 2002, p. 403-410.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Investigations on the laccase-catalyzed polymerization of lignin model compounds using size-exclusion HPLC

AU - Rittstieg, K.

AU - Suurnäkki, Anna

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AB - The reaction of laccase from Trametes hirsuta with monomeric and dimeric lignin model compounds was studied with special attention to the formation of polymeric reaction products. Different HPLC techniques were evaluated for the analysis of the reaction products. Methods requiring a solvent change from aqueous to organic phase were found not to be adequate for the analysis of product mixtures since evaporation over nitrogen led to the polymerization of reactive monomers and extraction with dichloromethane was not quantitative in all cases. Therefore, aqueous phase size-exclusion chromatography (SEC) at high pH was used to characterize reaction product mixtures. The product molecular weight distribution (MWD) after reaction with laccase was observed to be highly dependent on the type of model compound whereas the enzyme concentration had only a limited impact.

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