Pectinatus sottacetonis sp. nov., isolated from a commercial pickle spoilage tank

J.M. Caldwell (Corresponding Author), Riikka Juvonen, J. Brown, F. Breidt

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)

Abstract

A strictly anaerobic, Gram-stain-negative, non-spore-forming, motile bacterium, designated strain FSRU B0405T, was isolated from a commercial pickle spoilage tank and characterized by biochemical, physiological and molecular biological methods. Analyses of the 16S rRNA gene sequence of strain FSRU B0405T showed affiliation to the class Negativicutes in the phylum Firmicutes , with the closest relatives being the type strains of Pectinatus haikarae (96 %) and Pectinatus brassicae (95 %). In maximum-likelihood and neighbour-joining phylogenetic trees, strain FSRU B0405T clustered definitively (in 100 % of bootstrapped trees) within the genus Pectinatus , but not specifically with any characterized species within this genus. Strain FSRU B0405T was a slightly curved rod, varying from 3 to 30 µm in length, motile with a distinctive X-wise movement, having flagella only on the concave side of the cell. The isolate produced acetate and propionate from fructose and glucose as major metabolites similar to type strains of species of the genus Pectinatus. The major fatty acids were C11 : 0, C13 : 0, C15 : 0, C13 : 0 3-OH, C17 : 1 and C18 : 1ω11t. Strain FSRU B0405T differed from the pickle wastewater strain, Pectinatus brassicae TYT, due to its lack of susceptibility to vancomycin, acetoin production, growth temperature range, acid production from adonitol, erythritol, glycerol, inositol, lactose, maltose, mannose, ribose, salicin, sorbitol, trehalose and xylitol and lack of hydrolysis of milk. Strain FSRU B0405T could be differentiated from other species of the genus Pectinatus both phenotypically and genetically. The results indicate that strain FSRU B0405T represents a novel species of the genus Pectinatus, for which the name Pectinatus sottacetonis sp. nov. is proposed. The type strain is FSRU B0405T ( = ATCC BAA-2501T = VTT E-113163T). An emended description of the genus Pectinatus is also provided.
Original languageEnglish
Pages (from-to)3609-3616
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume63
Issue number10
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

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Pectinatus
pickles
spoilage
new species
biological method
aprepitant
milk
hydrolysis
metabolite
acetate
glucose
fatty acid
phylogenetics
Ribitol
Acetoin
wastewater
Erythritol
bacterium
Xylitol
gene

Cite this

@article{db16d3fa8da64b8dad5d9ba559a0e556,
title = "Pectinatus sottacetonis sp. nov., isolated from a commercial pickle spoilage tank",
abstract = "A strictly anaerobic, Gram-stain-negative, non-spore-forming, motile bacterium, designated strain FSRU B0405T, was isolated from a commercial pickle spoilage tank and characterized by biochemical, physiological and molecular biological methods. Analyses of the 16S rRNA gene sequence of strain FSRU B0405T showed affiliation to the class Negativicutes in the phylum Firmicutes , with the closest relatives being the type strains of Pectinatus haikarae (96 {\%}) and Pectinatus brassicae (95 {\%}). In maximum-likelihood and neighbour-joining phylogenetic trees, strain FSRU B0405T clustered definitively (in 100 {\%} of bootstrapped trees) within the genus Pectinatus , but not specifically with any characterized species within this genus. Strain FSRU B0405T was a slightly curved rod, varying from 3 to 30 µm in length, motile with a distinctive X-wise movement, having flagella only on the concave side of the cell. The isolate produced acetate and propionate from fructose and glucose as major metabolites similar to type strains of species of the genus Pectinatus. The major fatty acids were C11 : 0, C13 : 0, C15 : 0, C13 : 0 3-OH, C17 : 1 and C18 : 1ω11t. Strain FSRU B0405T differed from the pickle wastewater strain, Pectinatus brassicae TYT, due to its lack of susceptibility to vancomycin, acetoin production, growth temperature range, acid production from adonitol, erythritol, glycerol, inositol, lactose, maltose, mannose, ribose, salicin, sorbitol, trehalose and xylitol and lack of hydrolysis of milk. Strain FSRU B0405T could be differentiated from other species of the genus Pectinatus both phenotypically and genetically. The results indicate that strain FSRU B0405T represents a novel species of the genus Pectinatus, for which the name Pectinatus sottacetonis sp. nov. is proposed. The type strain is FSRU B0405T ( = ATCC BAA-2501T = VTT E-113163T). An emended description of the genus Pectinatus is also provided.",
author = "J.M. Caldwell and Riikka Juvonen and J. Brown and F. Breidt",
year = "2013",
doi = "10.1099/ijs.0.047886-0",
language = "English",
volume = "63",
pages = "3609--3616",
journal = "International Journal of Systematic and Evolutionary Microbiology",
issn = "1466-5026",
publisher = "Society for General Microbiology",
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}

Pectinatus sottacetonis sp. nov., isolated from a commercial pickle spoilage tank. / Caldwell, J.M. (Corresponding Author); Juvonen, Riikka; Brown, J.; Breidt, F.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 63, No. 10, 2013, p. 3609-3616.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Pectinatus sottacetonis sp. nov., isolated from a commercial pickle spoilage tank

AU - Caldwell, J.M.

AU - Juvonen, Riikka

AU - Brown, J.

AU - Breidt, F.

PY - 2013

Y1 - 2013

N2 - A strictly anaerobic, Gram-stain-negative, non-spore-forming, motile bacterium, designated strain FSRU B0405T, was isolated from a commercial pickle spoilage tank and characterized by biochemical, physiological and molecular biological methods. Analyses of the 16S rRNA gene sequence of strain FSRU B0405T showed affiliation to the class Negativicutes in the phylum Firmicutes , with the closest relatives being the type strains of Pectinatus haikarae (96 %) and Pectinatus brassicae (95 %). In maximum-likelihood and neighbour-joining phylogenetic trees, strain FSRU B0405T clustered definitively (in 100 % of bootstrapped trees) within the genus Pectinatus , but not specifically with any characterized species within this genus. Strain FSRU B0405T was a slightly curved rod, varying from 3 to 30 µm in length, motile with a distinctive X-wise movement, having flagella only on the concave side of the cell. The isolate produced acetate and propionate from fructose and glucose as major metabolites similar to type strains of species of the genus Pectinatus. The major fatty acids were C11 : 0, C13 : 0, C15 : 0, C13 : 0 3-OH, C17 : 1 and C18 : 1ω11t. Strain FSRU B0405T differed from the pickle wastewater strain, Pectinatus brassicae TYT, due to its lack of susceptibility to vancomycin, acetoin production, growth temperature range, acid production from adonitol, erythritol, glycerol, inositol, lactose, maltose, mannose, ribose, salicin, sorbitol, trehalose and xylitol and lack of hydrolysis of milk. Strain FSRU B0405T could be differentiated from other species of the genus Pectinatus both phenotypically and genetically. The results indicate that strain FSRU B0405T represents a novel species of the genus Pectinatus, for which the name Pectinatus sottacetonis sp. nov. is proposed. The type strain is FSRU B0405T ( = ATCC BAA-2501T = VTT E-113163T). An emended description of the genus Pectinatus is also provided.

AB - A strictly anaerobic, Gram-stain-negative, non-spore-forming, motile bacterium, designated strain FSRU B0405T, was isolated from a commercial pickle spoilage tank and characterized by biochemical, physiological and molecular biological methods. Analyses of the 16S rRNA gene sequence of strain FSRU B0405T showed affiliation to the class Negativicutes in the phylum Firmicutes , with the closest relatives being the type strains of Pectinatus haikarae (96 %) and Pectinatus brassicae (95 %). In maximum-likelihood and neighbour-joining phylogenetic trees, strain FSRU B0405T clustered definitively (in 100 % of bootstrapped trees) within the genus Pectinatus , but not specifically with any characterized species within this genus. Strain FSRU B0405T was a slightly curved rod, varying from 3 to 30 µm in length, motile with a distinctive X-wise movement, having flagella only on the concave side of the cell. The isolate produced acetate and propionate from fructose and glucose as major metabolites similar to type strains of species of the genus Pectinatus. The major fatty acids were C11 : 0, C13 : 0, C15 : 0, C13 : 0 3-OH, C17 : 1 and C18 : 1ω11t. Strain FSRU B0405T differed from the pickle wastewater strain, Pectinatus brassicae TYT, due to its lack of susceptibility to vancomycin, acetoin production, growth temperature range, acid production from adonitol, erythritol, glycerol, inositol, lactose, maltose, mannose, ribose, salicin, sorbitol, trehalose and xylitol and lack of hydrolysis of milk. Strain FSRU B0405T could be differentiated from other species of the genus Pectinatus both phenotypically and genetically. The results indicate that strain FSRU B0405T represents a novel species of the genus Pectinatus, for which the name Pectinatus sottacetonis sp. nov. is proposed. The type strain is FSRU B0405T ( = ATCC BAA-2501T = VTT E-113163T). An emended description of the genus Pectinatus is also provided.

U2 - 10.1099/ijs.0.047886-0

DO - 10.1099/ijs.0.047886-0

M3 - Article

VL - 63

SP - 3609

EP - 3616

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

SN - 1466-5026

IS - 10

ER -