Saccharomyces cerevisiae cells secreting an Aspergillus niger β-galactosidase grow on whey permeate

Vijay Kumar; Sundaram Ramakrishnan; Tuula Teeri; Jonathan Knowles; Brian Hartley

Saccharomyces cerevisiae cells secreting an Aspergillus niger β-galactosidase grow on whey permeate

Vijay Kumar, Sundaram Ramakrishnan, Tuula Teeri, Jonathan Knowles, Brian Hartley

VTT Technical Research Centre of Finland

Research output: Contribution to journal › Article › Scientific › peer-review

71 Citations (Scopus)

Abstract

We describe the construction of a lactose-utilizing Saccharomyces cerevisiae that expresses the cDNA for a secreted, thermostable β-galactosidase (lacA) from Aspergillus niger. Yeast cells expressing the lacA gene from the yeast ADH1 promoter on a multicopy plasmid secrete up to 40% of the total P-galactosidase activity into the growth medium. The secreted product is extensively N-glycosylated, and cells expressing the lacA gene grow on whey permeate (4% w/v lactose) with a doubling time of 1.6 hours. Such strains may offer a solution to the increasing problem of waste whey disposal.

Original language	English
Pages (from-to)	82-85
Journal	Bio/Technology
Volume	10
Issue number	1
Publication status	Published - 1992
MoE publication type	A1 Journal article-refereed

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https://www.nature.com/articles/nbt0192-82

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title = "Saccharomyces cerevisiae cells secreting an Aspergillus niger β-galactosidase grow on whey permeate",

abstract = "We describe the construction of a lactose-utilizing Saccharomyces cerevisiae that expresses the cDNA for a secreted, thermostable β-galactosidase (lacA) from Aspergillus niger. Yeast cells expressing the lacA gene from the yeast ADH1 promoter on a multicopy plasmid secrete up to 40% of the total P-galactosidase activity into the growth medium. The secreted product is extensively N-glycosylated, and cells expressing the lacA gene grow on whey permeate (4% w/v lactose) with a doubling time of 1.6 hours. Such strains may offer a solution to the increasing problem of waste whey disposal.",

author = "Vijay Kumar and Sundaram Ramakrishnan and Tuula Teeri and Jonathan Knowles and Brian Hartley",

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T1 - Saccharomyces cerevisiae cells secreting an Aspergillus niger β-galactosidase grow on whey permeate

AU - Kumar, Vijay

AU - Ramakrishnan, Sundaram

AU - Teeri, Tuula

AU - Knowles, Jonathan

AU - Hartley, Brian

PY - 1992

Y1 - 1992

N2 - We describe the construction of a lactose-utilizing Saccharomyces cerevisiae that expresses the cDNA for a secreted, thermostable β-galactosidase (lacA) from Aspergillus niger. Yeast cells expressing the lacA gene from the yeast ADH1 promoter on a multicopy plasmid secrete up to 40% of the total P-galactosidase activity into the growth medium. The secreted product is extensively N-glycosylated, and cells expressing the lacA gene grow on whey permeate (4% w/v lactose) with a doubling time of 1.6 hours. Such strains may offer a solution to the increasing problem of waste whey disposal.

AB - We describe the construction of a lactose-utilizing Saccharomyces cerevisiae that expresses the cDNA for a secreted, thermostable β-galactosidase (lacA) from Aspergillus niger. Yeast cells expressing the lacA gene from the yeast ADH1 promoter on a multicopy plasmid secrete up to 40% of the total P-galactosidase activity into the growth medium. The secreted product is extensively N-glycosylated, and cells expressing the lacA gene grow on whey permeate (4% w/v lactose) with a doubling time of 1.6 hours. Such strains may offer a solution to the increasing problem of waste whey disposal.

M3 - Article

VL - 10

SP - 82

EP - 85

JO - Nature Biotechnology

JF - Nature Biotechnology

SN - 1087-0156

IS - 1

ER -