Abstract
We describe the construction of a lactose-utilizing Saccharomyces cerevisiae that expresses the cDNA for a secreted, thermostable β-galactosidase (lacA) from Aspergillus niger. Yeast cells expressing the lacA gene from the yeast ADH1 promoter on a multicopy plasmid secrete up to 40% of the total P-galactosidase activity into the growth medium. The secreted product is extensively N-glycosylated, and cells expressing the lacA gene grow on whey permeate (4% w/v lactose) with a doubling time of 1.6 hours. Such strains may offer a solution to the increasing problem of waste whey disposal.
Original language | English |
---|---|
Pages (from-to) | 82-85 |
Journal | Bio/Technology |
Volume | 10 |
Issue number | 1 |
Publication status | Published - 1992 |
MoE publication type | A1 Journal article-refereed |