Abstract
We describe the construction of a lactose-utilizing Saccharomyces cerevisiae that expresses the cDNA for a secreted, thermostable β-galactosidase (lacA) from Aspergillus niger. Yeast cells expressing the lacA gene from the yeast ADH1 promoter on a multicopy plasmid secrete up to 40% of the total P-galactosidase activity into the growth medium. The secreted product is extensively N-glycosylated, and cells expressing the lacA gene grow on whey permeate (4% w/v lactose) with a doubling time of 1.6 hours. Such strains may offer a solution to the increasing problem of waste whey disposal.
| Original language | English |
|---|---|
| Pages (from-to) | 82-85 |
| Number of pages | 4 |
| Journal | Bio/Technology |
| Volume | 10 |
| Issue number | 1 |
| Publication status | Published - 1992 |
| MoE publication type | A1 Journal article-refereed |
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Kumar, V., Ramakrishnan, S., Teeri, T., Knowles, J., & Hartley, B. (1992). Saccharomyces cerevisiae cells secreting an Aspergillus niger β-galactosidase grow on whey permeate. Bio/Technology, 10(1), 82-85. https://www.nature.com/articles/nbt0192-82