Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 82-85 |
| Number of pages | 4 |
| Journal | Bio/Technology |
| Volume | 10 |
| Issue number | 1 |
| Publication status | Published - 1992 |
| MoE publication type | A1 Journal article-refereed |
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Saccharomyces cerevisiae cells secreting an Aspergillus niger β-galactosidase grow on whey permeate. / Kumar, Vijay; Ramakrishnan, Sundaram; Teeri, Tuula; Knowles, Jonathan; Hartley, Brian.
In: Bio/Technology, Vol. 10, No. 1, 1992, p. 82-85.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Saccharomyces cerevisiae cells secreting an Aspergillus niger β-galactosidase grow on whey permeate
AU - Kumar, Vijay
AU - Ramakrishnan, Sundaram
AU - Teeri, Tuula
AU - Knowles, Jonathan
AU - Hartley, Brian
PY - 1992
Y1 - 1992
N2 - We describe the construction of a lactose-utilizing Saccharomyces cerevisiae that expresses the cDNA for a secreted, thermostable β-galactosidase (lacA) from Aspergillus niger. Yeast cells expressing the lacA gene from the yeast ADH1 promoter on a multicopy plasmid secrete up to 40% of the total P-galactosidase activity into the growth medium. The secreted product is extensively N-glycosylated, and cells expressing the lacA gene grow on whey permeate (4% w/v lactose) with a doubling time of 1.6 hours. Such strains may offer a solution to the increasing problem of waste whey disposal.
AB - We describe the construction of a lactose-utilizing Saccharomyces cerevisiae that expresses the cDNA for a secreted, thermostable β-galactosidase (lacA) from Aspergillus niger. Yeast cells expressing the lacA gene from the yeast ADH1 promoter on a multicopy plasmid secrete up to 40% of the total P-galactosidase activity into the growth medium. The secreted product is extensively N-glycosylated, and cells expressing the lacA gene grow on whey permeate (4% w/v lactose) with a doubling time of 1.6 hours. Such strains may offer a solution to the increasing problem of waste whey disposal.
M3 - Article
VL - 10
SP - 82
EP - 85
JO - Nature Biotechnology
JF - Nature Biotechnology
SN - 1087-0156
IS - 1
ER -