We describe the construction of a lactose-utilizing Saccharomyces cerevisiae that expresses the cDNA for a secreted, thermostable β-galactosidase (lacA) from Aspergillus niger. Yeast cells expressing the lacA gene from the yeast ADH1 promoter on a multicopy plasmid secrete up to 40% of the total P-galactosidase activity into the growth medium. The secreted product is extensively N-glycosylated, and cells expressing the lacA gene grow on whey permeate (4% w/v lactose) with a doubling time of 1.6 hours. Such strains may offer a solution to the increasing problem of waste whey disposal.
|Number of pages||4|
|Publication status||Published - 1992|
|MoE publication type||A1 Journal article-refereed|
Kumar, V., Ramakrishnan, S., Teeri, T., Knowles, J., & Hartley, B. (1992). Saccharomyces cerevisiae cells secreting an Aspergillus niger β-galactosidase grow on whey permeate. Bio/Technology, 10(1), 82-85. https://www.nature.com/articles/nbt0192-82