Saccharomyces cerevisiae cells secreting an Aspergillus niger β-galactosidase grow on whey permeate

Vijay Kumar, Sundaram Ramakrishnan, Tuula Teeri, Jonathan Knowles, Brian Hartley

Research output: Contribution to journalArticleScientificpeer-review

68 Citations (Scopus)

Abstract

We describe the construction of a lactose-utilizing Saccharomyces cerevisiae that expresses the cDNA for a secreted, thermostable β-galactosidase (lacA) from Aspergillus niger. Yeast cells expressing the lacA gene from the yeast ADH1 promoter on a multicopy plasmid secrete up to 40% of the total P-galactosidase activity into the growth medium. The secreted product is extensively N-glycosylated, and cells expressing the lacA gene grow on whey permeate (4% w/v lactose) with a doubling time of 1.6 hours. Such strains may offer a solution to the increasing problem of waste whey disposal.
Original languageEnglish
Pages (from-to)82-85
Number of pages4
JournalBio/Technology
Volume10
Issue number1
Publication statusPublished - 1992
MoE publication typeA1 Journal article-refereed

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Galactosidases
Aspergillus niger
Aspergillus
Lactose
Yeast
Saccharomyces cerevisiae
Yeasts
Genes
Cells
Plasmids
Complementary DNA
Waste disposal
Growth
Whey

Cite this

Kumar, V., Ramakrishnan, S., Teeri, T., Knowles, J., & Hartley, B. (1992). Saccharomyces cerevisiae cells secreting an Aspergillus niger β-galactosidase grow on whey permeate. Bio/Technology, 10(1), 82-85.
Kumar, Vijay ; Ramakrishnan, Sundaram ; Teeri, Tuula ; Knowles, Jonathan ; Hartley, Brian. / Saccharomyces cerevisiae cells secreting an Aspergillus niger β-galactosidase grow on whey permeate. In: Bio/Technology. 1992 ; Vol. 10, No. 1. pp. 82-85.
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abstract = "We describe the construction of a lactose-utilizing Saccharomyces cerevisiae that expresses the cDNA for a secreted, thermostable β-galactosidase (lacA) from Aspergillus niger. Yeast cells expressing the lacA gene from the yeast ADH1 promoter on a multicopy plasmid secrete up to 40{\%} of the total P-galactosidase activity into the growth medium. The secreted product is extensively N-glycosylated, and cells expressing the lacA gene grow on whey permeate (4{\%} w/v lactose) with a doubling time of 1.6 hours. Such strains may offer a solution to the increasing problem of waste whey disposal.",
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Kumar, V, Ramakrishnan, S, Teeri, T, Knowles, J & Hartley, B 1992, 'Saccharomyces cerevisiae cells secreting an Aspergillus niger β-galactosidase grow on whey permeate', Bio/Technology, vol. 10, no. 1, pp. 82-85.

Saccharomyces cerevisiae cells secreting an Aspergillus niger β-galactosidase grow on whey permeate. / Kumar, Vijay; Ramakrishnan, Sundaram; Teeri, Tuula; Knowles, Jonathan; Hartley, Brian.

In: Bio/Technology, Vol. 10, No. 1, 1992, p. 82-85.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Saccharomyces cerevisiae cells secreting an Aspergillus niger β-galactosidase grow on whey permeate

AU - Kumar, Vijay

AU - Ramakrishnan, Sundaram

AU - Teeri, Tuula

AU - Knowles, Jonathan

AU - Hartley, Brian

PY - 1992

Y1 - 1992

N2 - We describe the construction of a lactose-utilizing Saccharomyces cerevisiae that expresses the cDNA for a secreted, thermostable β-galactosidase (lacA) from Aspergillus niger. Yeast cells expressing the lacA gene from the yeast ADH1 promoter on a multicopy plasmid secrete up to 40% of the total P-galactosidase activity into the growth medium. The secreted product is extensively N-glycosylated, and cells expressing the lacA gene grow on whey permeate (4% w/v lactose) with a doubling time of 1.6 hours. Such strains may offer a solution to the increasing problem of waste whey disposal.

AB - We describe the construction of a lactose-utilizing Saccharomyces cerevisiae that expresses the cDNA for a secreted, thermostable β-galactosidase (lacA) from Aspergillus niger. Yeast cells expressing the lacA gene from the yeast ADH1 promoter on a multicopy plasmid secrete up to 40% of the total P-galactosidase activity into the growth medium. The secreted product is extensively N-glycosylated, and cells expressing the lacA gene grow on whey permeate (4% w/v lactose) with a doubling time of 1.6 hours. Such strains may offer a solution to the increasing problem of waste whey disposal.

M3 - Article

VL - 10

SP - 82

EP - 85

JO - Nature Biotechnology

JF - Nature Biotechnology

SN - 1087-0156

IS - 1

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Kumar V, Ramakrishnan S, Teeri T, Knowles J, Hartley B. Saccharomyces cerevisiae cells secreting an Aspergillus niger β-galactosidase grow on whey permeate. Bio/Technology. 1992;10(1):82-85.