Isoelectric focusing electrophoresis of lignin

Marja-Leena Niku-Paavola

doi:10.1016/0003-2697(91)90362-W

Isoelectric focusing electrophoresis of lignin

Marja-Leena Niku-Paavola

VTT Technical Research Centre of Finland

Research output: Contribution to journal › Article › Scientific › peer-review

10 Citations (Scopus)

Abstract

Isoelectric focusing is introduced as a technique for the analysis of macromolecular lignin. The analysis is performed in a pH gradient from 3.5 to 10. Separated lignin fragments are visualized under uv light or by silver staining. The method can be used to distinguish between differently processed lignin preparations and to identify their components. Even the slight modification resulting from attack by ligninolytic enzymes could be detected.

Original language	English
Pages (from-to)	101 - 103
Number of pages	3
Journal	Analytical Biochemistry
Volume	197
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(91)90362-W
Publication status	Published - 1991
MoE publication type	A1 Journal article-refereed

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title = "Isoelectric focusing electrophoresis of lignin",

abstract = "Isoelectric focusing is introduced as a technique for the analysis of macromolecular lignin. The analysis is performed in a pH gradient from 3.5 to 10. Separated lignin fragments are visualized under uv light or by silver staining. The method can be used to distinguish between differently processed lignin preparations and to identify their components. Even the slight modification resulting from attack by ligninolytic enzymes could be detected.",

author = "Marja-Leena Niku-Paavola",

note = "Project code: BIO1022",

year = "1991",

doi = "10.1016/0003-2697(91)90362-W",

language = "English",

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pages = "101 -- 103",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press",

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T1 - Isoelectric focusing electrophoresis of lignin

AU - Niku-Paavola, Marja-Leena

N1 - Project code: BIO1022

PY - 1991

Y1 - 1991

N2 - Isoelectric focusing is introduced as a technique for the analysis of macromolecular lignin. The analysis is performed in a pH gradient from 3.5 to 10. Separated lignin fragments are visualized under uv light or by silver staining. The method can be used to distinguish between differently processed lignin preparations and to identify their components. Even the slight modification resulting from attack by ligninolytic enzymes could be detected.

AB - Isoelectric focusing is introduced as a technique for the analysis of macromolecular lignin. The analysis is performed in a pH gradient from 3.5 to 10. Separated lignin fragments are visualized under uv light or by silver staining. The method can be used to distinguish between differently processed lignin preparations and to identify their components. Even the slight modification resulting from attack by ligninolytic enzymes could be detected.

U2 - 10.1016/0003-2697(91)90362-W

DO - 10.1016/0003-2697(91)90362-W

M3 - Article

VL - 197

SP - 101

EP - 103

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 1

ER -