Isoelectric focusing electrophoresis of lignin

Marja-Leena Niku-Paavola

Research output: Contribution to journalArticleScientificpeer-review

9 Citations (Scopus)

Abstract

Isoelectric focusing is introduced as a technique for the analysis of macromolecular lignin. The analysis is performed in a pH gradient from 3.5 to 10. Separated lignin fragments are visualized under uv light or by silver staining. The method can be used to distinguish between differently processed lignin preparations and to identify their components. Even the slight modification resulting from attack by ligninolytic enzymes could be detected.

Original languageEnglish
Pages (from-to)101 - 103
Number of pages3
JournalAnalytical Biochemistry
Volume197
Issue number1
DOIs
Publication statusPublished - 1991
MoE publication typeA1 Journal article-refereed

Fingerprint

Lignin
Isoelectric Focusing
Electrophoresis
Silver Staining
Proton-Motive Force
Silver
Light
Enzymes

Cite this

Niku-Paavola, Marja-Leena. / Isoelectric focusing electrophoresis of lignin. In: Analytical Biochemistry. 1991 ; Vol. 197, No. 1. pp. 101 - 103.
@article{d398f7f98878441697da97513f596d3f,
title = "Isoelectric focusing electrophoresis of lignin",
abstract = "Isoelectric focusing is introduced as a technique for the analysis of macromolecular lignin. The analysis is performed in a pH gradient from 3.5 to 10. Separated lignin fragments are visualized under uv light or by silver staining. The method can be used to distinguish between differently processed lignin preparations and to identify their components. Even the slight modification resulting from attack by ligninolytic enzymes could be detected.",
author = "Marja-Leena Niku-Paavola",
note = "Project code: BIO1022",
year = "1991",
doi = "10.1016/0003-2697(91)90362-W",
language = "English",
volume = "197",
pages = "101 -- 103",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press",
number = "1",

}

Isoelectric focusing electrophoresis of lignin. / Niku-Paavola, Marja-Leena.

In: Analytical Biochemistry, Vol. 197, No. 1, 1991, p. 101 - 103.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Isoelectric focusing electrophoresis of lignin

AU - Niku-Paavola, Marja-Leena

N1 - Project code: BIO1022

PY - 1991

Y1 - 1991

N2 - Isoelectric focusing is introduced as a technique for the analysis of macromolecular lignin. The analysis is performed in a pH gradient from 3.5 to 10. Separated lignin fragments are visualized under uv light or by silver staining. The method can be used to distinguish between differently processed lignin preparations and to identify their components. Even the slight modification resulting from attack by ligninolytic enzymes could be detected.

AB - Isoelectric focusing is introduced as a technique for the analysis of macromolecular lignin. The analysis is performed in a pH gradient from 3.5 to 10. Separated lignin fragments are visualized under uv light or by silver staining. The method can be used to distinguish between differently processed lignin preparations and to identify their components. Even the slight modification resulting from attack by ligninolytic enzymes could be detected.

U2 - 10.1016/0003-2697(91)90362-W

DO - 10.1016/0003-2697(91)90362-W

M3 - Article

VL - 197

SP - 101

EP - 103

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 1

ER -