Isoelectric focusing electrophoresis of lignin

Marja-Leena Niku-Paavola

Research output: Contribution to journalArticleScientificpeer-review

9 Citations (Scopus)

Abstract

Isoelectric focusing is introduced as a technique for the analysis of macromolecular lignin. The analysis is performed in a pH gradient from 3.5 to 10. Separated lignin fragments are visualized under uv light or by silver staining. The method can be used to distinguish between differently processed lignin preparations and to identify their components. Even the slight modification resulting from attack by ligninolytic enzymes could be detected.

Original languageEnglish
Pages (from-to)101 - 103
Number of pages3
JournalAnalytical Biochemistry
Volume197
Issue number1
DOIs
Publication statusPublished - 1991
MoE publication typeA1 Journal article-refereed

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