Abstract
Isoelectric focusing is introduced as a technique for the analysis of macromolecular lignin. The analysis is performed in a pH gradient from 3.5 to 10. Separated lignin fragments are visualized under uv light or by silver staining. The method can be used to distinguish between differently processed lignin preparations and to identify their components. Even the slight modification resulting from attack by ligninolytic enzymes could be detected.
| Original language | English |
|---|---|
| Pages (from-to) | 101 - 103 |
| Number of pages | 3 |
| Journal | Analytical Biochemistry |
| Volume | 197 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1991 |
| MoE publication type | A1 Journal article-refereed |
Fingerprint
Cite this
}
Isoelectric focusing electrophoresis of lignin. / Niku-Paavola, Marja-Leena.
In: Analytical Biochemistry, Vol. 197, No. 1, 1991, p. 101 - 103.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Isoelectric focusing electrophoresis of lignin
AU - Niku-Paavola, Marja-Leena
N1 - Project code: BIO1022
PY - 1991
Y1 - 1991
N2 - Isoelectric focusing is introduced as a technique for the analysis of macromolecular lignin. The analysis is performed in a pH gradient from 3.5 to 10. Separated lignin fragments are visualized under uv light or by silver staining. The method can be used to distinguish between differently processed lignin preparations and to identify their components. Even the slight modification resulting from attack by ligninolytic enzymes could be detected.
AB - Isoelectric focusing is introduced as a technique for the analysis of macromolecular lignin. The analysis is performed in a pH gradient from 3.5 to 10. Separated lignin fragments are visualized under uv light or by silver staining. The method can be used to distinguish between differently processed lignin preparations and to identify their components. Even the slight modification resulting from attack by ligninolytic enzymes could be detected.
U2 - 10.1016/0003-2697(91)90362-W
DO - 10.1016/0003-2697(91)90362-W
M3 - Article
VL - 197
SP - 101
EP - 103
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 1
ER -