Isoelectric focusing electrophoresis of lignin

Marja-Leena Niku-Paavola

doi:10.1016/0003-2697(91)90362-W

Isoelectric focusing electrophoresis of lignin

Marja-Leena Niku-Paavola

VTT Technical Research Centre of Finland

Research output: Contribution to journal › Article

9 Citations (Scopus)

Abstract

Isoelectric focusing is introduced as a technique for the analysis of macromolecular lignin. The analysis is performed in a pH gradient from 3.5 to 10. Separated lignin fragments are visualized under uv light or by silver staining. The method can be used to distinguish between differently processed lignin preparations and to identify their components. Even the slight modification resulting from attack by ligninolytic enzymes could be detected.

Original language	English
Pages (from-to)	101 - 103
Number of pages	3
Journal	Analytical Biochemistry
Volume	197
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(91)90362-W
Publication status	Published - 1991
MoE publication type	A1 Journal article-refereed

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Niku-Paavola, M-L. (1991). Isoelectric focusing electrophoresis of lignin. Analytical Biochemistry, 197(1), 101 - 103. https://doi.org/10.1016/0003-2697(91)90362-W

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abstract = "Isoelectric focusing is introduced as a technique for the analysis of macromolecular lignin. The analysis is performed in a pH gradient from 3.5 to 10. Separated lignin fragments are visualized under uv light or by silver staining. The method can be used to distinguish between differently processed lignin preparations and to identify their components. Even the slight modification resulting from attack by ligninolytic enzymes could be detected.",

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AB - Isoelectric focusing is introduced as a technique for the analysis of macromolecular lignin. The analysis is performed in a pH gradient from 3.5 to 10. Separated lignin fragments are visualized under uv light or by silver staining. The method can be used to distinguish between differently processed lignin preparations and to identify their components. Even the slight modification resulting from attack by ligninolytic enzymes could be detected.

U2 - 10.1016/0003-2697(91)90362-W

DO - 10.1016/0003-2697(91)90362-W

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10.1016/0003-2697(91)90362-W