Isolation and automated ribotyping of Mycobacterium lentiflavum from drinking water distribution system and clinical specimens

Irina Tsitko (Corresponding Author), Riitta Rahkila, Outi Priha, Terhi Ali-Vehmas, Zewdu Terefework, Hanna Soini, Mirja S. Salkinoja-Salonen

Research output: Contribution to journalArticleScientificpeer-review

18 Citations (Scopus)

Abstract

Automated ribotyping as a tool for identifying of nontuberculous mycobacteria was evaluated. We created a database comprising of riboprints of 60 strains, representing 32 species of nontuberculous mycobacteria. It was shown that combined ribopatterns generated after digestion with EcoRI and PvuII were distinguishable between species of both slow-growing and rapid-growing mycobacteria. The findings were in good agreement with the 16S rRNA gene sequencing results, allowing correct identification of Mycobacterium lentiflavum isolated from clinical specimens and from biofilms growing in public water distribution system. The automated ribotyping was powerful in discriminating between M. lentiflavum and closely related species M. simiae and M. palustre. Mycobacterium lentiflavum strains from drinking water biofilms were resistant to two to four antimycobacterial drugs. The drinking water distribution system may, thus, be a source of nontuberculous mycobacteria resistant to multiple drugs.
Original languageEnglish
Pages (from-to)236-243
Number of pages8
JournalFEMS Microbiology Letters
Volume256
Issue number2
DOIs
Publication statusPublished - 2006
MoE publication typeA1 Journal article-refereed

Keywords

  • Mycobacterium lentiflavum
  • ribotyping
  • drinking water
  • biofilm

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