Isolation and characterization of the HFBIII hydrophobin of Trichoderma reesei

Eija Rintala, Markus Linder, Tiina Nakari-Setälä

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

Hydrophobins are surface active proteins produced by filamentous fungi. They have a role in fungal growth as structural components and in the interaction of fungi with their environment. Due to their physico-chemical characteristics they are very interesting proteins and suited also for various applications. We have been characterizing the hydrophobins of T. reesei on molecular and functional level. Previously we have reported characterization of the HFBI and HFBII hydrophobins. Recently, we have isolated a third hydrophobin, HFBIII, from T. reesei. The hfb3 gene was cloned based on internal peptide sequences obtained for HFBIII found in the supernatant of lactose cultures. HFBIII is a classII hydrophobin and very similar to both HFBI and HFBII the most specific feature of it being an extra cysteine residue between the regular 4th and 5th cysteines. Expression of hfb3 was studied and revealed that it is expressed similar to hfb2 on complex plat materials and lactose but the expression levels are lower. To characterize HFBIII at a protein level, a T. reesei strain overexpressing HFBIII in a HFBII deletion background was constructed. A purification prodedure for HFBIII was developed. HFBIII is largely retained in the mycelium in vegetative cultures. Our findings show that different treatments are needed for removal of HFBIII from the mycelium than for HFBI, which can be found in the cell walls of glucose-grown mycelia. This can indicate different structural roles for the two proteins.
Original languageEnglish
Title of host publication8th European Conference on Fungal Genetics
Subtitle of host publicationBook of Abstracts
PublisherVienna University of Technology
Publication statusPublished - 2006
Event8th European Conference on Fungal Genetics - Vienna, Austria
Duration: 8 Apr 200611 Apr 2006

Conference

Conference8th European Conference on Fungal Genetics
CountryAustria
CityVienna
Period8/04/0611/04/06

Fingerprint

Trichoderma
Mycelium
Lactose
Cysteine
Fungi
Proteins
Cell Wall
Membrane Proteins
Glucose
Peptides
Growth
Genes
1-(heptafluorobutyryl)imidazole

Cite this

Rintala, E., Linder, M., & Nakari-Setälä, T. (2006). Isolation and characterization of the HFBIII hydrophobin of Trichoderma reesei. In 8th European Conference on Fungal Genetics: Book of Abstracts [VIIp-9 ] Vienna University of Technology.
Rintala, Eija ; Linder, Markus ; Nakari-Setälä, Tiina. / Isolation and characterization of the HFBIII hydrophobin of Trichoderma reesei. 8th European Conference on Fungal Genetics: Book of Abstracts. Vienna University of Technology, 2006.
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abstract = "Hydrophobins are surface active proteins produced by filamentous fungi. They have a role in fungal growth as structural components and in the interaction of fungi with their environment. Due to their physico-chemical characteristics they are very interesting proteins and suited also for various applications. We have been characterizing the hydrophobins of T. reesei on molecular and functional level. Previously we have reported characterization of the HFBI and HFBII hydrophobins. Recently, we have isolated a third hydrophobin, HFBIII, from T. reesei. The hfb3 gene was cloned based on internal peptide sequences obtained for HFBIII found in the supernatant of lactose cultures. HFBIII is a classII hydrophobin and very similar to both HFBI and HFBII the most specific feature of it being an extra cysteine residue between the regular 4th and 5th cysteines. Expression of hfb3 was studied and revealed that it is expressed similar to hfb2 on complex plat materials and lactose but the expression levels are lower. To characterize HFBIII at a protein level, a T. reesei strain overexpressing HFBIII in a HFBII deletion background was constructed. A purification prodedure for HFBIII was developed. HFBIII is largely retained in the mycelium in vegetative cultures. Our findings show that different treatments are needed for removal of HFBIII from the mycelium than for HFBI, which can be found in the cell walls of glucose-grown mycelia. This can indicate different structural roles for the two proteins.",
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Rintala, E, Linder, M & Nakari-Setälä, T 2006, Isolation and characterization of the HFBIII hydrophobin of Trichoderma reesei. in 8th European Conference on Fungal Genetics: Book of Abstracts., VIIp-9 , Vienna University of Technology, 8th European Conference on Fungal Genetics, Vienna, Austria, 8/04/06.

Isolation and characterization of the HFBIII hydrophobin of Trichoderma reesei. / Rintala, Eija; Linder, Markus; Nakari-Setälä, Tiina.

8th European Conference on Fungal Genetics: Book of Abstracts. Vienna University of Technology, 2006. VIIp-9 .

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

TY - CHAP

T1 - Isolation and characterization of the HFBIII hydrophobin of Trichoderma reesei

AU - Rintala, Eija

AU - Linder, Markus

AU - Nakari-Setälä, Tiina

N1 - Poster

PY - 2006

Y1 - 2006

N2 - Hydrophobins are surface active proteins produced by filamentous fungi. They have a role in fungal growth as structural components and in the interaction of fungi with their environment. Due to their physico-chemical characteristics they are very interesting proteins and suited also for various applications. We have been characterizing the hydrophobins of T. reesei on molecular and functional level. Previously we have reported characterization of the HFBI and HFBII hydrophobins. Recently, we have isolated a third hydrophobin, HFBIII, from T. reesei. The hfb3 gene was cloned based on internal peptide sequences obtained for HFBIII found in the supernatant of lactose cultures. HFBIII is a classII hydrophobin and very similar to both HFBI and HFBII the most specific feature of it being an extra cysteine residue between the regular 4th and 5th cysteines. Expression of hfb3 was studied and revealed that it is expressed similar to hfb2 on complex plat materials and lactose but the expression levels are lower. To characterize HFBIII at a protein level, a T. reesei strain overexpressing HFBIII in a HFBII deletion background was constructed. A purification prodedure for HFBIII was developed. HFBIII is largely retained in the mycelium in vegetative cultures. Our findings show that different treatments are needed for removal of HFBIII from the mycelium than for HFBI, which can be found in the cell walls of glucose-grown mycelia. This can indicate different structural roles for the two proteins.

AB - Hydrophobins are surface active proteins produced by filamentous fungi. They have a role in fungal growth as structural components and in the interaction of fungi with their environment. Due to their physico-chemical characteristics they are very interesting proteins and suited also for various applications. We have been characterizing the hydrophobins of T. reesei on molecular and functional level. Previously we have reported characterization of the HFBI and HFBII hydrophobins. Recently, we have isolated a third hydrophobin, HFBIII, from T. reesei. The hfb3 gene was cloned based on internal peptide sequences obtained for HFBIII found in the supernatant of lactose cultures. HFBIII is a classII hydrophobin and very similar to both HFBI and HFBII the most specific feature of it being an extra cysteine residue between the regular 4th and 5th cysteines. Expression of hfb3 was studied and revealed that it is expressed similar to hfb2 on complex plat materials and lactose but the expression levels are lower. To characterize HFBIII at a protein level, a T. reesei strain overexpressing HFBIII in a HFBII deletion background was constructed. A purification prodedure for HFBIII was developed. HFBIII is largely retained in the mycelium in vegetative cultures. Our findings show that different treatments are needed for removal of HFBIII from the mycelium than for HFBI, which can be found in the cell walls of glucose-grown mycelia. This can indicate different structural roles for the two proteins.

M3 - Conference abstract in proceedings

BT - 8th European Conference on Fungal Genetics

PB - Vienna University of Technology

ER -

Rintala E, Linder M, Nakari-Setälä T. Isolation and characterization of the HFBIII hydrophobin of Trichoderma reesei. In 8th European Conference on Fungal Genetics: Book of Abstracts. Vienna University of Technology. 2006. VIIp-9