Isolation and preliminary characterization of plasmids in Bacillus badius

Anu Jalanko; Matti Leisola; Veli Kauppinen

doi:10.1016/0168-1656(86)90024-6

Isolation and preliminary characterization of plasmids in Bacillus badius

Anu Jalanko, Matti Leisola (Corresponding Author), Veli Kauppinen

VTT Technical Research Centre of Finland

Research output: Contribution to journal › Article › Scientific › peer-review

Abstract

The presence of urease and NADPH-specific glutamate dehydrogenase (GDH) coding plasmid in Bacillus badius was suggested by the loss of enzyme activites with a mitomycin C treatment and by the presence of 3 plasmids in urease-active strains compared to 2 plasmids in urease-negative strains. Furthermore two-dimensional protein gel electrophoresis showed that the urease-positive strains had some additional proteins compared to urease-negative strains. The two common plasmids had a size of 14.7 kb and 5.9 kb. The plasmid that was missing in the urease-negative strains had a size of 44.0 kb. The plasmids were purified and restriction map for AvaI, Bam HI and EcoRI was constructed for each plasmid. Hybridization tests showed that all three plasmids in Bacillus badius were independent entities.

Original language	English
Pages (from-to)	181-187
Journal	Journal of Biotechnology
Volume	4
Issue number	4
DOIs	https://doi.org/10.1016/0168-1656(86)90024-6
Publication status	Published - 1986
MoE publication type	A1 Journal article-refereed

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title = "Isolation and preliminary characterization of plasmids in Bacillus badius",

abstract = "The presence of urease and NADPH-specific glutamate dehydrogenase (GDH) coding plasmid in Bacillus badius was suggested by the loss of enzyme activites with a mitomycin C treatment and by the presence of 3 plasmids in urease-active strains compared to 2 plasmids in urease-negative strains. Furthermore two-dimensional protein gel electrophoresis showed that the urease-positive strains had some additional proteins compared to urease-negative strains. The two common plasmids had a size of 14.7 kb and 5.9 kb. The plasmid that was missing in the urease-negative strains had a size of 44.0 kb. The plasmids were purified and restriction map for AvaI, Bam HI and EcoRI was constructed for each plasmid. Hybridization tests showed that all three plasmids in Bacillus badius were independent entities.",

author = "Anu Jalanko and Matti Leisola and Veli Kauppinen",

year = "1986",

doi = "10.1016/0168-1656(86)90024-6",

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pages = "181--187",

journal = "Journal of Biotechnology",

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T1 - Isolation and preliminary characterization of plasmids in Bacillus badius

AU - Jalanko, Anu

AU - Leisola, Matti

AU - Kauppinen, Veli

PY - 1986

Y1 - 1986

N2 - The presence of urease and NADPH-specific glutamate dehydrogenase (GDH) coding plasmid in Bacillus badius was suggested by the loss of enzyme activites with a mitomycin C treatment and by the presence of 3 plasmids in urease-active strains compared to 2 plasmids in urease-negative strains. Furthermore two-dimensional protein gel electrophoresis showed that the urease-positive strains had some additional proteins compared to urease-negative strains. The two common plasmids had a size of 14.7 kb and 5.9 kb. The plasmid that was missing in the urease-negative strains had a size of 44.0 kb. The plasmids were purified and restriction map for AvaI, Bam HI and EcoRI was constructed for each plasmid. Hybridization tests showed that all three plasmids in Bacillus badius were independent entities.

AB - The presence of urease and NADPH-specific glutamate dehydrogenase (GDH) coding plasmid in Bacillus badius was suggested by the loss of enzyme activites with a mitomycin C treatment and by the presence of 3 plasmids in urease-active strains compared to 2 plasmids in urease-negative strains. Furthermore two-dimensional protein gel electrophoresis showed that the urease-positive strains had some additional proteins compared to urease-negative strains. The two common plasmids had a size of 14.7 kb and 5.9 kb. The plasmid that was missing in the urease-negative strains had a size of 44.0 kb. The plasmids were purified and restriction map for AvaI, Bam HI and EcoRI was constructed for each plasmid. Hybridization tests showed that all three plasmids in Bacillus badius were independent entities.

U2 - 10.1016/0168-1656(86)90024-6

DO - 10.1016/0168-1656(86)90024-6

M3 - Article

SN - 0168-1656

VL - 4

SP - 181

EP - 187

JO - Journal of Biotechnology

JF - Journal of Biotechnology

IS - 4

ER -

Isolation and preliminary characterization of plasmids in Bacillus badius

Abstract

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