Isolation and preliminary characterization of plasmids in Bacillus badius

Anu Jalanko, Matti Leisola (Corresponding Author), Veli Kauppinen

Research output: Contribution to journalArticleScientificpeer-review

Abstract

The presence of urease and NADPH-specific glutamate dehydrogenase (GDH) coding plasmid in Bacillus badius was suggested by the loss of enzyme activites with a mitomycin C treatment and by the presence of 3 plasmids in urease-active strains compared to 2 plasmids in urease-negative strains. Furthermore two-dimensional protein gel electrophoresis showed that the urease-positive strains had some additional proteins compared to urease-negative strains. The two common plasmids had a size of 14.7 kb and 5.9 kb. The plasmid that was missing in the urease-negative strains had a size of 44.0 kb. The plasmids were purified and restriction map for AvaI, Bam HI and EcoRI was constructed for each plasmid. Hybridization tests showed that all three plasmids in Bacillus badius were independent entities.

Original languageEnglish
Pages (from-to)181 - 187
Number of pages7
JournalJournal of Biotechnology
Volume4
Issue number4
DOIs
Publication statusPublished - 1986
MoE publication typeNot Eligible

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Bacilli
Bacillus
Urease
Plasmids
Proteins
Electrophoresis
Gels
Glutamate Dehydrogenase
Enzymes
Electrophoresis, Gel, Two-Dimensional
Mitomycin
NADP

Cite this

Jalanko, Anu ; Leisola, Matti ; Kauppinen, Veli. / Isolation and preliminary characterization of plasmids in Bacillus badius. In: Journal of Biotechnology. 1986 ; Vol. 4, No. 4. pp. 181 - 187.
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Isolation and preliminary characterization of plasmids in Bacillus badius. / Jalanko, Anu; Leisola, Matti (Corresponding Author); Kauppinen, Veli.

In: Journal of Biotechnology, Vol. 4, No. 4, 1986, p. 181 - 187.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Isolation and preliminary characterization of plasmids in Bacillus badius

AU - Jalanko, Anu

AU - Leisola, Matti

AU - Kauppinen, Veli

PY - 1986

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AB - The presence of urease and NADPH-specific glutamate dehydrogenase (GDH) coding plasmid in Bacillus badius was suggested by the loss of enzyme activites with a mitomycin C treatment and by the presence of 3 plasmids in urease-active strains compared to 2 plasmids in urease-negative strains. Furthermore two-dimensional protein gel electrophoresis showed that the urease-positive strains had some additional proteins compared to urease-negative strains. The two common plasmids had a size of 14.7 kb and 5.9 kb. The plasmid that was missing in the urease-negative strains had a size of 44.0 kb. The plasmids were purified and restriction map for AvaI, Bam HI and EcoRI was constructed for each plasmid. Hybridization tests showed that all three plasmids in Bacillus badius were independent entities.

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DO - 10.1016/0168-1656(86)90024-6

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