Isolation and structural analysis of the laccase gene from the lignin degrading fungus Phlebia radiata

Markku Saloheimo (Corresponding Author), Marja-Leena Niku-Paavola, Jonathan Knowles

    Research output: Contribution to journalArticleScientificpeer-review

    122 Citations (Scopus)

    Abstract

    We have isolated and characterized a gene coding for the laccase of the lignin-degrading fungus Phlebia radiata. The gene has nine introns and recognizable fungal promoter elements. Sequences homologous to the consensus eukaryotic heat-shock regulatory element can be found in the promoter. RNA hybridization results indicate that this gene is regulated at the transcriptional level. The derived laccase amino acid sequence shows homology to plant ascorbate oxidases, suggesting that the basic structure of the laccase is similar to the three-fold repeated β-barrel of the ascorbate oxidases. Potential copper ligands and a residue carrying the prosthetic group pyrroloquinoline quinone (PQQ) in the laccase protein can be identified by homology. The intron/exon structure of the laccase gene suggests that this protein could have evolved by exon shuffling.

    Original languageEnglish
    Pages (from-to)1537 - 1544
    Number of pages8
    JournalJournal of General Microbiology
    Volume137
    Issue number7
    DOIs
    Publication statusPublished - 1991
    MoE publication typeA1 Journal article-refereed

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