Isolation and structural analysis of the laccase gene from the lignin degrading fungus Phlebia radiata

Markku Saloheimo (Corresponding Author), Marja-Leena Niku-Paavola, Jonathan Knowles

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

We have isolated and characterized a gene coding for the laccase of the lignin-degrading fungus Phlebia radiata. The gene has nine introns and recognizable fungal promoter elements. Sequences homologous to the consensus eukaryotic heat-shock regulatory element can be found in the promoter. RNA hybridization results indicate that this gene is regulated at the transcriptional level. The derived laccase amino acid sequence shows homology to plant ascorbate oxidases, suggesting that the basic structure of the laccase is similar to the three-fold repeated β-barrel of the ascorbate oxidases. Potential copper ligands and a residue carrying the prosthetic group pyrroloquinoline quinone (PQQ) in the laccase protein can be identified by homology. The intron/exon structure of the laccase gene suggests that this protein could have evolved by exon shuffling.

Original languageEnglish
Pages (from-to)1537 - 1544
Number of pages8
JournalJournal of General Microbiology
Volume137
Issue number7
DOIs
Publication statusPublished - 1991
MoE publication typeA1 Journal article-refereed

Fingerprint

Laccase
Lignin
Fungi
Ascorbate Oxidase
Genes
Introns
Exons
PQQ Cofactor
Amino Acid Sequence Homology
Sequence Homology
Copper
Shock
Proteins
Hot Temperature
RNA
Ligands

Cite this

Saloheimo, Markku ; Niku-Paavola, Marja-Leena ; Knowles, Jonathan. / Isolation and structural analysis of the laccase gene from the lignin degrading fungus Phlebia radiata. In: Journal of General Microbiology. 1991 ; Vol. 137, No. 7. pp. 1537 - 1544.
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Isolation and structural analysis of the laccase gene from the lignin degrading fungus Phlebia radiata. / Saloheimo, Markku (Corresponding Author); Niku-Paavola, Marja-Leena; Knowles, Jonathan.

In: Journal of General Microbiology, Vol. 137, No. 7, 1991, p. 1537 - 1544.

Research output: Contribution to journalArticleScientificpeer-review

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AB - We have isolated and characterized a gene coding for the laccase of the lignin-degrading fungus Phlebia radiata. The gene has nine introns and recognizable fungal promoter elements. Sequences homologous to the consensus eukaryotic heat-shock regulatory element can be found in the promoter. RNA hybridization results indicate that this gene is regulated at the transcriptional level. The derived laccase amino acid sequence shows homology to plant ascorbate oxidases, suggesting that the basic structure of the laccase is similar to the three-fold repeated β-barrel of the ascorbate oxidases. Potential copper ligands and a residue carrying the prosthetic group pyrroloquinoline quinone (PQQ) in the laccase protein can be identified by homology. The intron/exon structure of the laccase gene suggests that this protein could have evolved by exon shuffling.

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