Isolation of Trichoderma reesei genes highly expressed on glucose-containing media

characterization of the tef1 gene encoding translation elongation factor 1α

Tiina Nakari, Edward Alatalo

Research output: Contribution to journalArticleScientificpeer-review

40 Citations (Scopus)

Abstract

Genes that are highly expressed on glucose-containing media were isolated from the filamentous fungus, Trichoderma reesei. A cDNA bank was prepared from glucose-grown fungus, the bank was screened with the same cDNA as a probe, and clones giving the strongest signal were isolated. This resulted in the isolation of previously uncharacterized genes. Five of the genes, representing the most abundant transcripts, corresponded to 1-3% of the total mRNA population and were clearly more highly expressed than the phosphoglycerate kinase-encoding gene (pgk1 ) of T. reesei. Based on sequence homology, one of the genes was identified as tef1, encoding translation elongation factor 1α (TEF). The T. reesei TEF is most related to the Mucor racemosus TEF3, showing an overall amino acid similarity of 85%. Interestingly, an exon of only 2 bp seems to be present in T. reesei tef1, comprising the first 2 bp of the Gly15 codon.

Original languageEnglish
Pages (from-to)313-318
Number of pages6
JournalGene
Volume136
Issue number1-2
DOIs
Publication statusPublished - 22 Dec 1993
MoE publication typeA1 Journal article-refereed

Fingerprint

Peptide Elongation Factor 1
Trichoderma
Glucose
Genes
Fungi
Complementary DNA
Phosphoglycerate Kinase
Mucor
Sequence Homology
Codon
Exons
Clone Cells
Amino Acids
Messenger RNA
Population

Keywords

  • abundant mRNA
  • cloning method
  • exon size
  • Filamentous fungi
  • gene expression

Cite this

@article{28e38608fa274492a3aa812f58e5e2d6,
title = "Isolation of Trichoderma reesei genes highly expressed on glucose-containing media: characterization of the tef1 gene encoding translation elongation factor 1α",
abstract = "Genes that are highly expressed on glucose-containing media were isolated from the filamentous fungus, Trichoderma reesei. A cDNA bank was prepared from glucose-grown fungus, the bank was screened with the same cDNA as a probe, and clones giving the strongest signal were isolated. This resulted in the isolation of previously uncharacterized genes. Five of the genes, representing the most abundant transcripts, corresponded to 1-3{\%} of the total mRNA population and were clearly more highly expressed than the phosphoglycerate kinase-encoding gene (pgk1 ) of T. reesei. Based on sequence homology, one of the genes was identified as tef1, encoding translation elongation factor 1α (TEF). The T. reesei TEF is most related to the Mucor racemosus TEF3, showing an overall amino acid similarity of 85{\%}. Interestingly, an exon of only 2 bp seems to be present in T. reesei tef1, comprising the first 2 bp of the Gly15 codon.",
keywords = "abundant mRNA, cloning method, exon size, Filamentous fungi, gene expression",
author = "Tiina Nakari and Edward Alatalo",
year = "1993",
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language = "English",
volume = "136",
pages = "313--318",
journal = "Gene",
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}

Isolation of Trichoderma reesei genes highly expressed on glucose-containing media : characterization of the tef1 gene encoding translation elongation factor 1α. / Nakari, Tiina; Alatalo, Edward.

In: Gene, Vol. 136, No. 1-2, 22.12.1993, p. 313-318.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

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T2 - characterization of the tef1 gene encoding translation elongation factor 1α

AU - Nakari, Tiina

AU - Alatalo, Edward

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Y1 - 1993/12/22

N2 - Genes that are highly expressed on glucose-containing media were isolated from the filamentous fungus, Trichoderma reesei. A cDNA bank was prepared from glucose-grown fungus, the bank was screened with the same cDNA as a probe, and clones giving the strongest signal were isolated. This resulted in the isolation of previously uncharacterized genes. Five of the genes, representing the most abundant transcripts, corresponded to 1-3% of the total mRNA population and were clearly more highly expressed than the phosphoglycerate kinase-encoding gene (pgk1 ) of T. reesei. Based on sequence homology, one of the genes was identified as tef1, encoding translation elongation factor 1α (TEF). The T. reesei TEF is most related to the Mucor racemosus TEF3, showing an overall amino acid similarity of 85%. Interestingly, an exon of only 2 bp seems to be present in T. reesei tef1, comprising the first 2 bp of the Gly15 codon.

AB - Genes that are highly expressed on glucose-containing media were isolated from the filamentous fungus, Trichoderma reesei. A cDNA bank was prepared from glucose-grown fungus, the bank was screened with the same cDNA as a probe, and clones giving the strongest signal were isolated. This resulted in the isolation of previously uncharacterized genes. Five of the genes, representing the most abundant transcripts, corresponded to 1-3% of the total mRNA population and were clearly more highly expressed than the phosphoglycerate kinase-encoding gene (pgk1 ) of T. reesei. Based on sequence homology, one of the genes was identified as tef1, encoding translation elongation factor 1α (TEF). The T. reesei TEF is most related to the Mucor racemosus TEF3, showing an overall amino acid similarity of 85%. Interestingly, an exon of only 2 bp seems to be present in T. reesei tef1, comprising the first 2 bp of the Gly15 codon.

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KW - cloning method

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