Isolation of Trichoderma reesei genes highly expressed on glucose-containing media: characterization of the tef1 gene encoding translation elongation factor 1α

Tiina Nakari, Edward Alatalo

    Research output: Contribution to journalArticleScientificpeer-review

    43 Citations (Scopus)

    Abstract

    Genes that are highly expressed on glucose-containing media were isolated from the filamentous fungus, Trichoderma reesei. A cDNA bank was prepared from glucose-grown fungus, the bank was screened with the same cDNA as a probe, and clones giving the strongest signal were isolated. This resulted in the isolation of previously uncharacterized genes. Five of the genes, representing the most abundant transcripts, corresponded to 1-3% of the total mRNA population and were clearly more highly expressed than the phosphoglycerate kinase-encoding gene (pgk1 ) of T. reesei. Based on sequence homology, one of the genes was identified as tef1, encoding translation elongation factor 1α (TEF). The T. reesei TEF is most related to the Mucor racemosus TEF3, showing an overall amino acid similarity of 85%. Interestingly, an exon of only 2 bp seems to be present in T. reesei tef1, comprising the first 2 bp of the Gly15 codon.

    Original languageEnglish
    Pages (from-to)313-318
    Number of pages6
    JournalGene
    Volume136
    Issue number1-2
    DOIs
    Publication statusPublished - 22 Dec 1993
    MoE publication typeA1 Journal article-refereed

    Keywords

    • abundant mRNA
    • cloning method
    • exon size
    • Filamentous fungi
    • gene expression

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