Isotachophoresis of β‐blockers in a capillary and on a poly(methyl methacrylate) chip

Pirkko Kriikku, Benedikt Graß, Ari Hokkanen, Ingmar Stuns, Heli Sirén (Corresponding Author)

    Research output: Contribution to journalArticleScientificpeer-review

    24 Citations (Scopus)

    Abstract

    Analysis of the β‐blockers oxprenolol, atenolol, timolol, propranolol, metoprolol, and acebutolol in human urine by a combination of isotachophoresis (ITP) and zone electrophoresis (ZE) was investigated. Methods were developed with a conventional capillary electrophoresis (CE) apparatus and a poly(methyl methacrylate) (PMMA) microchip system. With CE the separation of oxprenolol, atenolol, timolol, and acebutolol from a standard solution containing 5 μg/mL of each compound was accomplished by performing ZE with transient ITP. The electrolyte system consisted of 10 mM sodium morpholinoethane sulfonate (pH 5.5) and 0.1% methylhydroxyethylcellulose as the leading electrolyte and 30 mM ortho‐phosphoric acid (pH 2.0) as both the terminating and the ZE background electrolyte. With the microchip system the separation of oxprenolol and acebutolol from a standard solution containing 10 μg/mL of each compound was accomplished by a coupled‐channel ITP‐ZE device using the same leading electrolyte solution as the CE system but 5 mM glutamic acid (pH 3.4) as terminating and background electrolytes. The systems were used for analyses of patient urine samples. Water‐soluble hydrophilic matrix compounds were removed from the urine samples by solid‐phase extraction (SPE). Limits of quantification below 5 μg/mL could be achieved. The PMMA ITP‐ZE chip has not earlier been used for analyses of any drugs from urine samples.
    Original languageEnglish
    Pages (from-to)1687 - 1694
    Number of pages8
    JournalElectrophoresis
    Volume25
    Issue number10-11
    DOIs
    Publication statusPublished - 2004
    MoE publication typeA1 Journal article-refereed

    Fingerprint

    Isotachophoresis
    Polymethyl Methacrylate
    Electrolytes
    Acebutolol
    Oxprenolol
    Capillary electrophoresis
    Capillary Electrophoresis
    Electrophoresis
    Urine
    Timolol
    Atenolol
    Metoprolol
    Propranolol
    Glutamic Acid
    Sodium
    Equipment and Supplies
    Pharmaceutical Preparations

    Keywords

    • beta-Blockers
    • Capillary electrophoresis
    • In-line isotachophoresis-zone electrophoresis
    • Isotachophoresis
    • Miniaturization
    • Poly(methyl methacrylate)microchip
    • Solid-phase extraction

    Cite this

    Kriikku, Pirkko ; Graß, Benedikt ; Hokkanen, Ari ; Stuns, Ingmar ; Sirén, Heli. / Isotachophoresis of β‐blockers in a capillary and on a poly(methyl methacrylate) chip. In: Electrophoresis. 2004 ; Vol. 25, No. 10-11. pp. 1687 - 1694.
    @article{8d9f3b74db7e4130b524f500cf079b9e,
    title = "Isotachophoresis of β‐blockers in a capillary and on a poly(methyl methacrylate) chip",
    abstract = "Analysis of the β‐blockers oxprenolol, atenolol, timolol, propranolol, metoprolol, and acebutolol in human urine by a combination of isotachophoresis (ITP) and zone electrophoresis (ZE) was investigated. Methods were developed with a conventional capillary electrophoresis (CE) apparatus and a poly(methyl methacrylate) (PMMA) microchip system. With CE the separation of oxprenolol, atenolol, timolol, and acebutolol from a standard solution containing 5 μg/mL of each compound was accomplished by performing ZE with transient ITP. The electrolyte system consisted of 10 mM sodium morpholinoethane sulfonate (pH 5.5) and 0.1{\%} methylhydroxyethylcellulose as the leading electrolyte and 30 mM ortho‐phosphoric acid (pH 2.0) as both the terminating and the ZE background electrolyte. With the microchip system the separation of oxprenolol and acebutolol from a standard solution containing 10 μg/mL of each compound was accomplished by a coupled‐channel ITP‐ZE device using the same leading electrolyte solution as the CE system but 5 mM glutamic acid (pH 3.4) as terminating and background electrolytes. The systems were used for analyses of patient urine samples. Water‐soluble hydrophilic matrix compounds were removed from the urine samples by solid‐phase extraction (SPE). Limits of quantification below 5 μg/mL could be achieved. The PMMA ITP‐ZE chip has not earlier been used for analyses of any drugs from urine samples.",
    keywords = "beta-Blockers, Capillary electrophoresis, In-line isotachophoresis-zone electrophoresis, Isotachophoresis, Miniaturization, Poly(methyl methacrylate)microchip, Solid-phase extraction",
    author = "Pirkko Kriikku and Benedikt Gra{\ss} and Ari Hokkanen and Ingmar Stuns and Heli Sir{\'e}n",
    year = "2004",
    doi = "10.1002/elps.200305872",
    language = "English",
    volume = "25",
    pages = "1687 -- 1694",
    journal = "Electrophoresis",
    issn = "0173-0835",
    publisher = "Wiley-VCH Verlag",
    number = "10-11",

    }

    Isotachophoresis of β‐blockers in a capillary and on a poly(methyl methacrylate) chip. / Kriikku, Pirkko; Graß, Benedikt; Hokkanen, Ari; Stuns, Ingmar; Sirén, Heli (Corresponding Author).

    In: Electrophoresis, Vol. 25, No. 10-11, 2004, p. 1687 - 1694.

    Research output: Contribution to journalArticleScientificpeer-review

    TY - JOUR

    T1 - Isotachophoresis of β‐blockers in a capillary and on a poly(methyl methacrylate) chip

    AU - Kriikku, Pirkko

    AU - Graß, Benedikt

    AU - Hokkanen, Ari

    AU - Stuns, Ingmar

    AU - Sirén, Heli

    PY - 2004

    Y1 - 2004

    N2 - Analysis of the β‐blockers oxprenolol, atenolol, timolol, propranolol, metoprolol, and acebutolol in human urine by a combination of isotachophoresis (ITP) and zone electrophoresis (ZE) was investigated. Methods were developed with a conventional capillary electrophoresis (CE) apparatus and a poly(methyl methacrylate) (PMMA) microchip system. With CE the separation of oxprenolol, atenolol, timolol, and acebutolol from a standard solution containing 5 μg/mL of each compound was accomplished by performing ZE with transient ITP. The electrolyte system consisted of 10 mM sodium morpholinoethane sulfonate (pH 5.5) and 0.1% methylhydroxyethylcellulose as the leading electrolyte and 30 mM ortho‐phosphoric acid (pH 2.0) as both the terminating and the ZE background electrolyte. With the microchip system the separation of oxprenolol and acebutolol from a standard solution containing 10 μg/mL of each compound was accomplished by a coupled‐channel ITP‐ZE device using the same leading electrolyte solution as the CE system but 5 mM glutamic acid (pH 3.4) as terminating and background electrolytes. The systems were used for analyses of patient urine samples. Water‐soluble hydrophilic matrix compounds were removed from the urine samples by solid‐phase extraction (SPE). Limits of quantification below 5 μg/mL could be achieved. The PMMA ITP‐ZE chip has not earlier been used for analyses of any drugs from urine samples.

    AB - Analysis of the β‐blockers oxprenolol, atenolol, timolol, propranolol, metoprolol, and acebutolol in human urine by a combination of isotachophoresis (ITP) and zone electrophoresis (ZE) was investigated. Methods were developed with a conventional capillary electrophoresis (CE) apparatus and a poly(methyl methacrylate) (PMMA) microchip system. With CE the separation of oxprenolol, atenolol, timolol, and acebutolol from a standard solution containing 5 μg/mL of each compound was accomplished by performing ZE with transient ITP. The electrolyte system consisted of 10 mM sodium morpholinoethane sulfonate (pH 5.5) and 0.1% methylhydroxyethylcellulose as the leading electrolyte and 30 mM ortho‐phosphoric acid (pH 2.0) as both the terminating and the ZE background electrolyte. With the microchip system the separation of oxprenolol and acebutolol from a standard solution containing 10 μg/mL of each compound was accomplished by a coupled‐channel ITP‐ZE device using the same leading electrolyte solution as the CE system but 5 mM glutamic acid (pH 3.4) as terminating and background electrolytes. The systems were used for analyses of patient urine samples. Water‐soluble hydrophilic matrix compounds were removed from the urine samples by solid‐phase extraction (SPE). Limits of quantification below 5 μg/mL could be achieved. The PMMA ITP‐ZE chip has not earlier been used for analyses of any drugs from urine samples.

    KW - beta-Blockers

    KW - Capillary electrophoresis

    KW - In-line isotachophoresis-zone electrophoresis

    KW - Isotachophoresis

    KW - Miniaturization

    KW - Poly(methyl methacrylate)microchip

    KW - Solid-phase extraction

    U2 - 10.1002/elps.200305872

    DO - 10.1002/elps.200305872

    M3 - Article

    VL - 25

    SP - 1687

    EP - 1694

    JO - Electrophoresis

    JF - Electrophoresis

    SN - 0173-0835

    IS - 10-11

    ER -