Isotachophoresis of β‐blockers in a capillary and on a poly(methyl methacrylate) chip

Pirkko Kriikku, Benedikt Graß, Ari Hokkanen, Ingmar Stuns, Heli Sirén (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

24 Citations (Scopus)

Abstract

Analysis of the β‐blockers oxprenolol, atenolol, timolol, propranolol, metoprolol, and acebutolol in human urine by a combination of isotachophoresis (ITP) and zone electrophoresis (ZE) was investigated. Methods were developed with a conventional capillary electrophoresis (CE) apparatus and a poly(methyl methacrylate) (PMMA) microchip system. With CE the separation of oxprenolol, atenolol, timolol, and acebutolol from a standard solution containing 5 μg/mL of each compound was accomplished by performing ZE with transient ITP. The electrolyte system consisted of 10 mM sodium morpholinoethane sulfonate (pH 5.5) and 0.1% methylhydroxyethylcellulose as the leading electrolyte and 30 mM ortho‐phosphoric acid (pH 2.0) as both the terminating and the ZE background electrolyte. With the microchip system the separation of oxprenolol and acebutolol from a standard solution containing 10 μg/mL of each compound was accomplished by a coupled‐channel ITP‐ZE device using the same leading electrolyte solution as the CE system but 5 mM glutamic acid (pH 3.4) as terminating and background electrolytes. The systems were used for analyses of patient urine samples. Water‐soluble hydrophilic matrix compounds were removed from the urine samples by solid‐phase extraction (SPE). Limits of quantification below 5 μg/mL could be achieved. The PMMA ITP‐ZE chip has not earlier been used for analyses of any drugs from urine samples.
Original languageEnglish
Pages (from-to)1687 - 1694
Number of pages8
JournalElectrophoresis
Volume25
Issue number10-11
DOIs
Publication statusPublished - 2004
MoE publication typeA1 Journal article-refereed

Fingerprint

Isotachophoresis
Polymethyl Methacrylate
Electrolytes
Acebutolol
Oxprenolol
Capillary electrophoresis
Capillary Electrophoresis
Electrophoresis
Urine
Timolol
Atenolol
Metoprolol
Propranolol
Glutamic Acid
Sodium
Equipment and Supplies
Pharmaceutical Preparations

Keywords

  • beta-Blockers
  • Capillary electrophoresis
  • In-line isotachophoresis-zone electrophoresis
  • Isotachophoresis
  • Miniaturization
  • Poly(methyl methacrylate)microchip
  • Solid-phase extraction

Cite this

Kriikku, Pirkko ; Graß, Benedikt ; Hokkanen, Ari ; Stuns, Ingmar ; Sirén, Heli. / Isotachophoresis of β‐blockers in a capillary and on a poly(methyl methacrylate) chip. In: Electrophoresis. 2004 ; Vol. 25, No. 10-11. pp. 1687 - 1694.
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abstract = "Analysis of the β‐blockers oxprenolol, atenolol, timolol, propranolol, metoprolol, and acebutolol in human urine by a combination of isotachophoresis (ITP) and zone electrophoresis (ZE) was investigated. Methods were developed with a conventional capillary electrophoresis (CE) apparatus and a poly(methyl methacrylate) (PMMA) microchip system. With CE the separation of oxprenolol, atenolol, timolol, and acebutolol from a standard solution containing 5 μg/mL of each compound was accomplished by performing ZE with transient ITP. The electrolyte system consisted of 10 mM sodium morpholinoethane sulfonate (pH 5.5) and 0.1{\%} methylhydroxyethylcellulose as the leading electrolyte and 30 mM ortho‐phosphoric acid (pH 2.0) as both the terminating and the ZE background electrolyte. With the microchip system the separation of oxprenolol and acebutolol from a standard solution containing 10 μg/mL of each compound was accomplished by a coupled‐channel ITP‐ZE device using the same leading electrolyte solution as the CE system but 5 mM glutamic acid (pH 3.4) as terminating and background electrolytes. The systems were used for analyses of patient urine samples. Water‐soluble hydrophilic matrix compounds were removed from the urine samples by solid‐phase extraction (SPE). Limits of quantification below 5 μg/mL could be achieved. The PMMA ITP‐ZE chip has not earlier been used for analyses of any drugs from urine samples.",
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Isotachophoresis of β‐blockers in a capillary and on a poly(methyl methacrylate) chip. / Kriikku, Pirkko; Graß, Benedikt; Hokkanen, Ari; Stuns, Ingmar; Sirén, Heli (Corresponding Author).

In: Electrophoresis, Vol. 25, No. 10-11, 2004, p. 1687 - 1694.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Isotachophoresis of β‐blockers in a capillary and on a poly(methyl methacrylate) chip

AU - Kriikku, Pirkko

AU - Graß, Benedikt

AU - Hokkanen, Ari

AU - Stuns, Ingmar

AU - Sirén, Heli

PY - 2004

Y1 - 2004

N2 - Analysis of the β‐blockers oxprenolol, atenolol, timolol, propranolol, metoprolol, and acebutolol in human urine by a combination of isotachophoresis (ITP) and zone electrophoresis (ZE) was investigated. Methods were developed with a conventional capillary electrophoresis (CE) apparatus and a poly(methyl methacrylate) (PMMA) microchip system. With CE the separation of oxprenolol, atenolol, timolol, and acebutolol from a standard solution containing 5 μg/mL of each compound was accomplished by performing ZE with transient ITP. The electrolyte system consisted of 10 mM sodium morpholinoethane sulfonate (pH 5.5) and 0.1% methylhydroxyethylcellulose as the leading electrolyte and 30 mM ortho‐phosphoric acid (pH 2.0) as both the terminating and the ZE background electrolyte. With the microchip system the separation of oxprenolol and acebutolol from a standard solution containing 10 μg/mL of each compound was accomplished by a coupled‐channel ITP‐ZE device using the same leading electrolyte solution as the CE system but 5 mM glutamic acid (pH 3.4) as terminating and background electrolytes. The systems were used for analyses of patient urine samples. Water‐soluble hydrophilic matrix compounds were removed from the urine samples by solid‐phase extraction (SPE). Limits of quantification below 5 μg/mL could be achieved. The PMMA ITP‐ZE chip has not earlier been used for analyses of any drugs from urine samples.

AB - Analysis of the β‐blockers oxprenolol, atenolol, timolol, propranolol, metoprolol, and acebutolol in human urine by a combination of isotachophoresis (ITP) and zone electrophoresis (ZE) was investigated. Methods were developed with a conventional capillary electrophoresis (CE) apparatus and a poly(methyl methacrylate) (PMMA) microchip system. With CE the separation of oxprenolol, atenolol, timolol, and acebutolol from a standard solution containing 5 μg/mL of each compound was accomplished by performing ZE with transient ITP. The electrolyte system consisted of 10 mM sodium morpholinoethane sulfonate (pH 5.5) and 0.1% methylhydroxyethylcellulose as the leading electrolyte and 30 mM ortho‐phosphoric acid (pH 2.0) as both the terminating and the ZE background electrolyte. With the microchip system the separation of oxprenolol and acebutolol from a standard solution containing 10 μg/mL of each compound was accomplished by a coupled‐channel ITP‐ZE device using the same leading electrolyte solution as the CE system but 5 mM glutamic acid (pH 3.4) as terminating and background electrolytes. The systems were used for analyses of patient urine samples. Water‐soluble hydrophilic matrix compounds were removed from the urine samples by solid‐phase extraction (SPE). Limits of quantification below 5 μg/mL could be achieved. The PMMA ITP‐ZE chip has not earlier been used for analyses of any drugs from urine samples.

KW - beta-Blockers

KW - Capillary electrophoresis

KW - In-line isotachophoresis-zone electrophoresis

KW - Isotachophoresis

KW - Miniaturization

KW - Poly(methyl methacrylate)microchip

KW - Solid-phase extraction

U2 - 10.1002/elps.200305872

DO - 10.1002/elps.200305872

M3 - Article

VL - 25

SP - 1687

EP - 1694

JO - Electrophoresis

JF - Electrophoresis

SN - 0173-0835

IS - 10-11

ER -