Jasmonate signaling involves the abscisic acid receptor PYL4 to regulate metabolic reprogramming in Arabidopsis and tobacco

Petri Lackman, Miguel González-Guzmán, Sofie Tilleman, Inês Carqueijeiro, Amparo Cuéllar Pérez, Tessa Moses, Mitsunori Seo, Yuri Kanno, Suvi T. Häkkinen, Marc C. E. Van Montagu (Corresponding Author), Johan M. Thevelein, Hannu Maaheimo, Kirsi-Marja Oksman-Caldentey, Pedro L. Rodriguez, Heiko Rischer, Alain Goossens (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

132 Citations (Scopus)

Abstract

The phytohormones jasmonates (JAs) constitute an important class of elicitors for many plant secondary metabolic pathways. However, JAs do not act independently but operate in complex networks with crosstalk to several other phytohormonal signaling pathways. Here, crosstalk was detected between the JA and abscisic acid (ABA) signaling pathways in the regulation of tobacco (Nicotiana tabacum) alkaloid biosynthesis. A tobacco gene from the PYR/PYL/RCAR family, NtPYL4, the expression of which is regulated by JAs, was found to encode a functional ABA receptor. NtPYL4 inhibited the type-2C protein phosphatases known to be key negative regulators of ABA signaling in an ABA-dependent manner. Overexpression of NtPYL4 in tobacco hairy roots caused a reprogramming of the cellular metabolism that resulted in a decreased alkaloid accumulation and conferred ABA sensitivity to the production of alkaloids. In contrast, the alkaloid biosynthetic pathway was not responsive to ABA in control tobacco roots. Functional analysis of the Arabidopsis (Arabidopsis thaliana) homologs of NtPYL4, PYL4 and PYL5, indicated that also in Arabidopsis altered PYL expression affected the JA response, both in terms of biomass and anthocyanin production. These findings define a connection between a component of the core ABA signaling pathway and the JA responses and contribute to the understanding of the role of JAs in balancing tradeoffs between growth and defense.
Original languageEnglish
Pages (from-to)5891-5896
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number14
DOIs
Publication statusPublished - 2011
MoE publication typeA1 Journal article-refereed

Keywords

  • nicotine
  • phenylpropanoid
  • primary metabolism
  • secondary metabolism
  • stress response

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    Lackman, P., González-Guzmán, M., Tilleman, S., Carqueijeiro, I., Cuéllar Pérez, A., Moses, T., Seo, M., Kanno, Y., Häkkinen, S. T., Van Montagu, M. C. E., Thevelein, J. M., Maaheimo, H., Oksman-Caldentey, K-M., Rodriguez, P. L., Rischer, H., & Goossens, A. (2011). Jasmonate signaling involves the abscisic acid receptor PYL4 to regulate metabolic reprogramming in Arabidopsis and tobacco. Proceedings of the National Academy of Sciences of the United States of America, 108(14), 5891-5896. https://doi.org/10.1073/pnas.1103010108