Jasmonates induce over-accumulation of methylputrescine and conjugated polyamines in Hyoscyamus muticus L. root cultures

S. Biondi (Corresponding Author), S. Fornale, Kirsi-Marja Oksman-Caldentey, M. Eeva, S. Agostani, N. Bagni

Research output: Contribution to journalArticleScientificpeer-review

50 Citations (Scopus)

Abstract

Jasmonic acid (JA) and its methyl ester (MeJA) at concentrations ranging from 0.001 to 10 μM provoked large increases in methylputrescine levels in normal and hairy roots of Hyoscyamus muticus L.; generally, levels of free putrescine and perchloric acid-soluble conjugated putrescine, spermidine and spermine also increased dramatically. More 14C-putrescine was formed when hairy roots were incubated with labelled ornithine than with arginine; conjugated 14C-putrescine was also rapidly formed. In accord with these results, ornithine decarboxylase (EC 4.1.1.17) activity was higher than that of arginine decarboxylase (EC 4.1.1.19), and MeJA enhanced these activities about two- and fourfold, respectively. Although treatment of root cultures with jasmonates enhanced precursor (putrescine, methylputrescine) levels and accumulation of secondary metabolites such as acid-soluble conjugated di-/polyamines, it provoked only modest increases in tropane alkaloid tissue levels.
Original languageEnglish
Pages (from-to)691 - 697
Number of pages7
JournalPlant Cell Reports
Volume19
Issue number7
DOIs
Publication statusPublished - 2000
MoE publication typeA1 Journal article-refereed

Fingerprint

Hyoscyamus muticus
jasmonic acid
putrescine
polyamines
arginine decarboxylase
ornithine decarboxylase
tropane alkaloids
perchloric acid
ornithine
spermine
spermidine
secondary metabolites
arginine
esters
acids

Keywords

  • Hairy roots
  • Hydroxycinnamoyl amides
  • Hyoscyamus muticus
  • Jasmonates
  • Methylputrescine

Cite this

@article{989f75985207478585c2f1e489301b97,
title = "Jasmonates induce over-accumulation of methylputrescine and conjugated polyamines in Hyoscyamus muticus L. root cultures",
abstract = "Jasmonic acid (JA) and its methyl ester (MeJA) at concentrations ranging from 0.001 to 10 μM provoked large increases in methylputrescine levels in normal and hairy roots of Hyoscyamus muticus L.; generally, levels of free putrescine and perchloric acid-soluble conjugated putrescine, spermidine and spermine also increased dramatically. More 14C-putrescine was formed when hairy roots were incubated with labelled ornithine than with arginine; conjugated 14C-putrescine was also rapidly formed. In accord with these results, ornithine decarboxylase (EC 4.1.1.17) activity was higher than that of arginine decarboxylase (EC 4.1.1.19), and MeJA enhanced these activities about two- and fourfold, respectively. Although treatment of root cultures with jasmonates enhanced precursor (putrescine, methylputrescine) levels and accumulation of secondary metabolites such as acid-soluble conjugated di-/polyamines, it provoked only modest increases in tropane alkaloid tissue levels.",
keywords = "Hairy roots, Hydroxycinnamoyl amides, Hyoscyamus muticus, Jasmonates, Methylputrescine",
author = "S. Biondi and S. Fornale and Kirsi-Marja Oksman-Caldentey and M. Eeva and S. Agostani and N. Bagni",
year = "2000",
doi = "10.1007/s002999900178",
language = "English",
volume = "19",
pages = "691 -- 697",
journal = "Plant Cell Reports",
issn = "0721-7714",
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Jasmonates induce over-accumulation of methylputrescine and conjugated polyamines in Hyoscyamus muticus L. root cultures. / Biondi, S. (Corresponding Author); Fornale, S.; Oksman-Caldentey, Kirsi-Marja; Eeva, M.; Agostani, S.; Bagni, N.

In: Plant Cell Reports, Vol. 19, No. 7, 2000, p. 691 - 697.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Jasmonates induce over-accumulation of methylputrescine and conjugated polyamines in Hyoscyamus muticus L. root cultures

AU - Biondi, S.

AU - Fornale, S.

AU - Oksman-Caldentey, Kirsi-Marja

AU - Eeva, M.

AU - Agostani, S.

AU - Bagni, N.

PY - 2000

Y1 - 2000

N2 - Jasmonic acid (JA) and its methyl ester (MeJA) at concentrations ranging from 0.001 to 10 μM provoked large increases in methylputrescine levels in normal and hairy roots of Hyoscyamus muticus L.; generally, levels of free putrescine and perchloric acid-soluble conjugated putrescine, spermidine and spermine also increased dramatically. More 14C-putrescine was formed when hairy roots were incubated with labelled ornithine than with arginine; conjugated 14C-putrescine was also rapidly formed. In accord with these results, ornithine decarboxylase (EC 4.1.1.17) activity was higher than that of arginine decarboxylase (EC 4.1.1.19), and MeJA enhanced these activities about two- and fourfold, respectively. Although treatment of root cultures with jasmonates enhanced precursor (putrescine, methylputrescine) levels and accumulation of secondary metabolites such as acid-soluble conjugated di-/polyamines, it provoked only modest increases in tropane alkaloid tissue levels.

AB - Jasmonic acid (JA) and its methyl ester (MeJA) at concentrations ranging from 0.001 to 10 μM provoked large increases in methylputrescine levels in normal and hairy roots of Hyoscyamus muticus L.; generally, levels of free putrescine and perchloric acid-soluble conjugated putrescine, spermidine and spermine also increased dramatically. More 14C-putrescine was formed when hairy roots were incubated with labelled ornithine than with arginine; conjugated 14C-putrescine was also rapidly formed. In accord with these results, ornithine decarboxylase (EC 4.1.1.17) activity was higher than that of arginine decarboxylase (EC 4.1.1.19), and MeJA enhanced these activities about two- and fourfold, respectively. Although treatment of root cultures with jasmonates enhanced precursor (putrescine, methylputrescine) levels and accumulation of secondary metabolites such as acid-soluble conjugated di-/polyamines, it provoked only modest increases in tropane alkaloid tissue levels.

KW - Hairy roots

KW - Hydroxycinnamoyl amides

KW - Hyoscyamus muticus

KW - Jasmonates

KW - Methylputrescine

U2 - 10.1007/s002999900178

DO - 10.1007/s002999900178

M3 - Article

VL - 19

SP - 691

EP - 697

JO - Plant Cell Reports

JF - Plant Cell Reports

SN - 0721-7714

IS - 7

ER -