Kinetic and stereochemical studies of manno-oligosaccharide hydrolysis catalysed by beta-mannanases from trichoderma reesei

Vesa Harjunpää (Corresponding Author), Teleman Anita, Matti Siika-aho, Torbjörn Drakenberg

Research output: Contribution to journalArticleScientificpeer-review

40 Citations (Scopus)

Abstract

The two β‐mannanases from Trichoderma reesei with pI of 4.6 and 5.4, respectively, have been characterised by NMR spectroscopy.
Following the kinetics of manno‐oligosaccharide degradation with complete progress‐curve analysis the stereospecificity and degradation pattern have been delineated. It was found that degradation of mannotriose and mannopentaose proceeds with retention of the anomeric configuration. Mannotriose degradation proceeds by almost random release of mannose.
For mannopentaose there is initially no mannose formed showing that only the two middle mannosidic linkages are attacked. Progress‐curve analysis shows that there is preference (70%) for cleavage of mannopentaose in such a way that mannobiose is released from the reducing end. The final product composition from the mannotriose degradation showed that transglycosylation has to be taken into account.
Model calculation and progress‐curve analysis showed that the transglycosylation rate is the fastest of all the rates in this system, 15 s‐1 compared with mannohexaose and mannotetraose hydrolysis rates of 2 s‐1 and mannotriose hydrolysis rate of 0.03 s‐1 at 50°C.
Original languageEnglish
Pages (from-to)278-283
JournalEuropean Journal of Biochemistry
Volume234
Issue number1
DOIs
Publication statusPublished - 1995
MoE publication typeA1 Journal article-refereed

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beta-Mannosidase
Trichoderma
Oligosaccharides
Hydrolysis
Degradation
Kinetics
Mannose
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance spectroscopy
mannotriose
mannopentaose
Chemical analysis

Cite this

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title = "Kinetic and stereochemical studies of manno-oligosaccharide hydrolysis catalysed by beta-mannanases from trichoderma reesei",
abstract = "The two β‐mannanases from Trichoderma reesei with pI of 4.6 and 5.4, respectively, have been characterised by NMR spectroscopy. Following the kinetics of manno‐oligosaccharide degradation with complete progress‐curve analysis the stereospecificity and degradation pattern have been delineated. It was found that degradation of mannotriose and mannopentaose proceeds with retention of the anomeric configuration. Mannotriose degradation proceeds by almost random release of mannose. For mannopentaose there is initially no mannose formed showing that only the two middle mannosidic linkages are attacked. Progress‐curve analysis shows that there is preference (70{\%}) for cleavage of mannopentaose in such a way that mannobiose is released from the reducing end. The final product composition from the mannotriose degradation showed that transglycosylation has to be taken into account. Model calculation and progress‐curve analysis showed that the transglycosylation rate is the fastest of all the rates in this system, 15 s‐1 compared with mannohexaose and mannotetraose hydrolysis rates of 2 s‐1 and mannotriose hydrolysis rate of 0.03 s‐1 at 50°C.",
author = "Vesa Harjunp{\"a}{\"a} and Teleman Anita and Matti Siika-aho and Torbj{\"o}rn Drakenberg",
year = "1995",
doi = "10.1111/j.1432-1033.1995.278_c.x",
language = "English",
volume = "234",
pages = "278--283",
journal = "FEBS Journal",
issn = "1742-464X",
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Kinetic and stereochemical studies of manno-oligosaccharide hydrolysis catalysed by beta-mannanases from trichoderma reesei. / Harjunpää, Vesa (Corresponding Author); Anita, Teleman; Siika-aho, Matti; Drakenberg, Torbjörn.

In: European Journal of Biochemistry, Vol. 234, No. 1, 1995, p. 278-283.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Kinetic and stereochemical studies of manno-oligosaccharide hydrolysis catalysed by beta-mannanases from trichoderma reesei

AU - Harjunpää, Vesa

AU - Anita, Teleman

AU - Siika-aho, Matti

AU - Drakenberg, Torbjörn

PY - 1995

Y1 - 1995

N2 - The two β‐mannanases from Trichoderma reesei with pI of 4.6 and 5.4, respectively, have been characterised by NMR spectroscopy. Following the kinetics of manno‐oligosaccharide degradation with complete progress‐curve analysis the stereospecificity and degradation pattern have been delineated. It was found that degradation of mannotriose and mannopentaose proceeds with retention of the anomeric configuration. Mannotriose degradation proceeds by almost random release of mannose. For mannopentaose there is initially no mannose formed showing that only the two middle mannosidic linkages are attacked. Progress‐curve analysis shows that there is preference (70%) for cleavage of mannopentaose in such a way that mannobiose is released from the reducing end. The final product composition from the mannotriose degradation showed that transglycosylation has to be taken into account. Model calculation and progress‐curve analysis showed that the transglycosylation rate is the fastest of all the rates in this system, 15 s‐1 compared with mannohexaose and mannotetraose hydrolysis rates of 2 s‐1 and mannotriose hydrolysis rate of 0.03 s‐1 at 50°C.

AB - The two β‐mannanases from Trichoderma reesei with pI of 4.6 and 5.4, respectively, have been characterised by NMR spectroscopy. Following the kinetics of manno‐oligosaccharide degradation with complete progress‐curve analysis the stereospecificity and degradation pattern have been delineated. It was found that degradation of mannotriose and mannopentaose proceeds with retention of the anomeric configuration. Mannotriose degradation proceeds by almost random release of mannose. For mannopentaose there is initially no mannose formed showing that only the two middle mannosidic linkages are attacked. Progress‐curve analysis shows that there is preference (70%) for cleavage of mannopentaose in such a way that mannobiose is released from the reducing end. The final product composition from the mannotriose degradation showed that transglycosylation has to be taken into account. Model calculation and progress‐curve analysis showed that the transglycosylation rate is the fastest of all the rates in this system, 15 s‐1 compared with mannohexaose and mannotetraose hydrolysis rates of 2 s‐1 and mannotriose hydrolysis rate of 0.03 s‐1 at 50°C.

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DO - 10.1111/j.1432-1033.1995.278_c.x

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