Abstract
reesei, cellobiohydrolase I (CBHI), was used as a model protein in the studies. To study the protein production kinetics in a controlled physiological state, a chemostat cultivation system was set up for the fungus. In the chemostat cultivations, the intracellular production rate of labelled CBHI and the accumulation rate of the protein into the culture medium was measured at different dilution rates, and the corresponding specific steady-state mRNA levels were determined. With this methodology we have been able to follow the time course of protein synthesis, glycosylation and transport into the medium,
and obtain information on the synthesis vs. secretion capacity of the fungus in relation to growth rate.
| Original language | English |
|---|---|
| Title of host publication | Abstracts from the 3rd ECFG |
| Subtitle of host publication | European Congress on Fungal Genetics |
| Publisher | New Prairie Press |
| DOIs | |
| Publication status | Published - 1996 |
| Event | 3rd European Fungal Conference on Fungal Genetics - Münster, Germany Duration: 27 Mar 1996 → 30 Mar 1996 |
Publication series
| Series | Fungal Genetics Reports |
|---|---|
| Number | 34 |
| Volume | 43 |
Conference
| Conference | 3rd European Fungal Conference on Fungal Genetics |
|---|---|
| Country | Germany |
| City | Münster |
| Period | 27/03/96 → 30/03/96 |
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Kinetics of Protein Secretion in Trichoderma Reesei. / Pakula, Tiina; Salonen, Katri; Uusitalo, Jaana; Saloheimo, Markku; Aarts, Robert J.; Penttilä, Merja.
Abstracts from the 3rd ECFG: European Congress on Fungal Genetics. New Prairie Press, 1996. (Fungal Genetics Reports; No. 34, Vol. 43).Research output: Chapter in Book/Report/Conference proceeding › Conference abstract in proceedings › Scientific
TY - CHAP
T1 - Kinetics of Protein Secretion in Trichoderma Reesei
AU - Pakula, Tiina
AU - Salonen, Katri
AU - Uusitalo, Jaana
AU - Saloheimo, Markku
AU - Aarts, Robert J.
AU - Penttilä, Merja
PY - 1996
Y1 - 1996
N2 - In order to obtain knowledge of the protein secretion process in filamentous fungi, and to study the rate-limiting steps in protein production, we have developed methodology for measuring the kinetics of protein production and secretion by metabolic labelling in Trichoderma. The synthesis of the 35S- labelled proteins and their transport into the growth medium was monitored by 2D gel electrophoresis and/or immunoprecipitation. The major cellulose of T.reesei, cellobiohydrolase I (CBHI), was used as a model protein in the studies. To study the protein production kinetics in a controlled physiological state, a chemostat cultivation system was set up for the fungus. In the chemostat cultivations, the intracellular production rate of labelled CBHI and the accumulation rate of the protein into the culture medium was measured at different dilution rates, and the corresponding specific steady-state mRNA levels were determined. With this methodology we have been able to follow the time course of protein synthesis, glycosylation and transport into the medium,and obtain information on the synthesis vs. secretion capacity of the fungus in relation to growth rate.
AB - In order to obtain knowledge of the protein secretion process in filamentous fungi, and to study the rate-limiting steps in protein production, we have developed methodology for measuring the kinetics of protein production and secretion by metabolic labelling in Trichoderma. The synthesis of the 35S- labelled proteins and their transport into the growth medium was monitored by 2D gel electrophoresis and/or immunoprecipitation. The major cellulose of T.reesei, cellobiohydrolase I (CBHI), was used as a model protein in the studies. To study the protein production kinetics in a controlled physiological state, a chemostat cultivation system was set up for the fungus. In the chemostat cultivations, the intracellular production rate of labelled CBHI and the accumulation rate of the protein into the culture medium was measured at different dilution rates, and the corresponding specific steady-state mRNA levels were determined. With this methodology we have been able to follow the time course of protein synthesis, glycosylation and transport into the medium,and obtain information on the synthesis vs. secretion capacity of the fungus in relation to growth rate.
U2 - 10.4148/1941-4765.1330
DO - 10.4148/1941-4765.1330
M3 - Conference abstract in proceedings
T3 - Fungal Genetics Reports
BT - Abstracts from the 3rd ECFG
PB - New Prairie Press
ER -