L-xylo-3-hexulose reductase is the missing link in the oxidoreductive pathway for D-galactose catabolism in filamentous fungi

Dominik Mojzita, S. Herold, B. Metz, B. Seiboth, Peter Richard (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

15 Citations (Scopus)

Abstract

In addition to the well established Leloir pathway for the catabolism of D-galactose in fungi, the oxidoreductive pathway has been recently identified. In this oxidoreductive pathway, D-galactose is converted via a series of NADPH-dependent reductions and NAD+-dependent oxidations into D-fructose. The pathway intermediates include galactitol, L-xylo-3-hexulose, and D-sorbitol. This study identified the missing link in the pathway, the L-xylo-3-hexulose reductase that catalyzes the conversion of L-xylo-3-hexulose to D-sorbitol. In Trichoderma reesei (Hypocrea jecorina) and Aspergillus niger, we identified the genes lxr4 and xhrA, respectively, that encode the L-xylo-3-hexulose reductases. The deletion of these genes resulted in no growth on galactitol and in reduced growth on D-galactose. The LXR4 was heterologously expressed, and the purified protein showed high specificity for L-xylo-3-hexulose with a Km = 2.0 ± 0.5 mM and a Vmax = 5.5 ± 1.0 units/mg. We also confirmed that the product of the LXR4 reaction is D-sorbitol.
Original languageEnglish
Pages (from-to)26010-26018
Number of pages8
JournalJournal of Biological Chemistry
Volume287
Issue number31
DOIs
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed

Fingerprint

Fungi
Galactose
Oxidoreductases
Sorbitol
Galactitol
Hypocrea
Genes
Trichoderma
Aspergillus niger
Aspergillus
Gene Deletion
Growth
Fructose
NADP
NAD
xylo-3-hexulose
Oxidation
Proteins

Keywords

  • Aspergillus niger
  • Trichoderma reesei
  • Hypocrea jecorina
  • D-galactose metabolism
  • D-galactose
  • L-xylo-3-hexulose
  • L-xylo-3-hexulose reductase
  • xhrA
  • lxr4

Cite this

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title = "L-xylo-3-hexulose reductase is the missing link in the oxidoreductive pathway for D-galactose catabolism in filamentous fungi",
abstract = "In addition to the well established Leloir pathway for the catabolism of D-galactose in fungi, the oxidoreductive pathway has been recently identified. In this oxidoreductive pathway, D-galactose is converted via a series of NADPH-dependent reductions and NAD+-dependent oxidations into D-fructose. The pathway intermediates include galactitol, L-xylo-3-hexulose, and D-sorbitol. This study identified the missing link in the pathway, the L-xylo-3-hexulose reductase that catalyzes the conversion of L-xylo-3-hexulose to D-sorbitol. In Trichoderma reesei (Hypocrea jecorina) and Aspergillus niger, we identified the genes lxr4 and xhrA, respectively, that encode the L-xylo-3-hexulose reductases. The deletion of these genes resulted in no growth on galactitol and in reduced growth on D-galactose. The LXR4 was heterologously expressed, and the purified protein showed high specificity for L-xylo-3-hexulose with a Km = 2.0 ± 0.5 mM and a Vmax = 5.5 ± 1.0 units/mg. We also confirmed that the product of the LXR4 reaction is D-sorbitol.",
keywords = "Aspergillus niger, Trichoderma reesei, Hypocrea jecorina, D-galactose metabolism, D-galactose, L-xylo-3-hexulose, L-xylo-3-hexulose reductase, xhrA, lxr4",
author = "Dominik Mojzita and S. Herold and B. Metz and B. Seiboth and Peter Richard",
year = "2012",
doi = "10.1074/jbc.M112.372755",
language = "English",
volume = "287",
pages = "26010--26018",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "31",

}

L-xylo-3-hexulose reductase is the missing link in the oxidoreductive pathway for D-galactose catabolism in filamentous fungi. / Mojzita, Dominik; Herold, S.; Metz, B.; Seiboth, B.; Richard, Peter (Corresponding Author).

In: Journal of Biological Chemistry, Vol. 287, No. 31, 2012, p. 26010-26018.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - L-xylo-3-hexulose reductase is the missing link in the oxidoreductive pathway for D-galactose catabolism in filamentous fungi

AU - Mojzita, Dominik

AU - Herold, S.

AU - Metz, B.

AU - Seiboth, B.

AU - Richard, Peter

PY - 2012

Y1 - 2012

N2 - In addition to the well established Leloir pathway for the catabolism of D-galactose in fungi, the oxidoreductive pathway has been recently identified. In this oxidoreductive pathway, D-galactose is converted via a series of NADPH-dependent reductions and NAD+-dependent oxidations into D-fructose. The pathway intermediates include galactitol, L-xylo-3-hexulose, and D-sorbitol. This study identified the missing link in the pathway, the L-xylo-3-hexulose reductase that catalyzes the conversion of L-xylo-3-hexulose to D-sorbitol. In Trichoderma reesei (Hypocrea jecorina) and Aspergillus niger, we identified the genes lxr4 and xhrA, respectively, that encode the L-xylo-3-hexulose reductases. The deletion of these genes resulted in no growth on galactitol and in reduced growth on D-galactose. The LXR4 was heterologously expressed, and the purified protein showed high specificity for L-xylo-3-hexulose with a Km = 2.0 ± 0.5 mM and a Vmax = 5.5 ± 1.0 units/mg. We also confirmed that the product of the LXR4 reaction is D-sorbitol.

AB - In addition to the well established Leloir pathway for the catabolism of D-galactose in fungi, the oxidoreductive pathway has been recently identified. In this oxidoreductive pathway, D-galactose is converted via a series of NADPH-dependent reductions and NAD+-dependent oxidations into D-fructose. The pathway intermediates include galactitol, L-xylo-3-hexulose, and D-sorbitol. This study identified the missing link in the pathway, the L-xylo-3-hexulose reductase that catalyzes the conversion of L-xylo-3-hexulose to D-sorbitol. In Trichoderma reesei (Hypocrea jecorina) and Aspergillus niger, we identified the genes lxr4 and xhrA, respectively, that encode the L-xylo-3-hexulose reductases. The deletion of these genes resulted in no growth on galactitol and in reduced growth on D-galactose. The LXR4 was heterologously expressed, and the purified protein showed high specificity for L-xylo-3-hexulose with a Km = 2.0 ± 0.5 mM and a Vmax = 5.5 ± 1.0 units/mg. We also confirmed that the product of the LXR4 reaction is D-sorbitol.

KW - Aspergillus niger

KW - Trichoderma reesei

KW - Hypocrea jecorina

KW - D-galactose metabolism

KW - D-galactose

KW - L-xylo-3-hexulose

KW - L-xylo-3-hexulose reductase

KW - xhrA

KW - lxr4

U2 - 10.1074/jbc.M112.372755

DO - 10.1074/jbc.M112.372755

M3 - Article

VL - 287

SP - 26010

EP - 26018

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 31

ER -