Laccase-catalysed functionalisation of TMP with tyramine

Stina Grönqvist (Corresponding Author), K. Rantanen, Raimo Alen, Maija-Liisa Mattinen, Johanna Buchert, Liisa Viikari

Research output: Contribution to journalArticleScientificpeer-review

44 Citations (Scopus)

Abstract

Modified wood fibres open new perspectives to create value-added products based on renewable raw materials. An interesting option is the targeted modification of fibre surfaces by oxidative enzymes. This two-stage functionalisation method consists of enzymatic activation of fibre surfaces followed by addition of radicalised compounds reacting preferentially by radical coupling. In this work, the activation of bleached and unbleached softwood TMPs with laccase isolated from Trametes hirsuta was studied. The formation and stability of the radicals were studied by EPR spectroscopy. The reaction of the radicals with 3-hydroxytyramine hydrochloride and the type of chemical linkages were investigated. EPR, ESCA and FTIR spectroscopy were used for analysis. Bleached TMP was radicalised more efficiently than unbleached TMP. The radicals were unstable, as 90% of them were quenched within a few hours. Their lifetime was, however, found to be adequately long for performing coupling reactions. Bonding of new compounds to pulps via radical reactions thus seems to be possible.
Original languageEnglish
Pages (from-to)503-508
Number of pages6
JournalHolzforschung
Volume60
Issue number5
DOIs
Publication statusPublished - 2006
MoE publication typeA1 Journal article-refereed

Fingerprint

Thymidine Monophosphate
Laccase
Tyramine
Paramagnetic resonance
Fibers
Chemical activation
Spectroscopy
Softwoods
Pulp
Dopamine
Wood
Raw materials
Enzymes

Keywords

  • Activation of fibres
  • EPR
  • ESCA (XPS)
  • FTIR
  • Functionalisation of fibres
  • Laccases
  • Lifetime of radicals
  • Surface modification

Cite this

Grönqvist, S., Rantanen, K., Alen, R., Mattinen, M-L., Buchert, J., & Viikari, L. (2006). Laccase-catalysed functionalisation of TMP with tyramine. Holzforschung, 60(5), 503-508. https://doi.org/10.1515/HF.2006.083
Grönqvist, Stina ; Rantanen, K. ; Alen, Raimo ; Mattinen, Maija-Liisa ; Buchert, Johanna ; Viikari, Liisa. / Laccase-catalysed functionalisation of TMP with tyramine. In: Holzforschung. 2006 ; Vol. 60, No. 5. pp. 503-508.
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Grönqvist, S, Rantanen, K, Alen, R, Mattinen, M-L, Buchert, J & Viikari, L 2006, 'Laccase-catalysed functionalisation of TMP with tyramine', Holzforschung, vol. 60, no. 5, pp. 503-508. https://doi.org/10.1515/HF.2006.083

Laccase-catalysed functionalisation of TMP with tyramine. / Grönqvist, Stina (Corresponding Author); Rantanen, K.; Alen, Raimo; Mattinen, Maija-Liisa; Buchert, Johanna; Viikari, Liisa.

In: Holzforschung, Vol. 60, No. 5, 2006, p. 503-508.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Laccase-catalysed functionalisation of TMP with tyramine

AU - Grönqvist, Stina

AU - Rantanen, K.

AU - Alen, Raimo

AU - Mattinen, Maija-Liisa

AU - Buchert, Johanna

AU - Viikari, Liisa

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N2 - Modified wood fibres open new perspectives to create value-added products based on renewable raw materials. An interesting option is the targeted modification of fibre surfaces by oxidative enzymes. This two-stage functionalisation method consists of enzymatic activation of fibre surfaces followed by addition of radicalised compounds reacting preferentially by radical coupling. In this work, the activation of bleached and unbleached softwood TMPs with laccase isolated from Trametes hirsuta was studied. The formation and stability of the radicals were studied by EPR spectroscopy. The reaction of the radicals with 3-hydroxytyramine hydrochloride and the type of chemical linkages were investigated. EPR, ESCA and FTIR spectroscopy were used for analysis. Bleached TMP was radicalised more efficiently than unbleached TMP. The radicals were unstable, as 90% of them were quenched within a few hours. Their lifetime was, however, found to be adequately long for performing coupling reactions. Bonding of new compounds to pulps via radical reactions thus seems to be possible.

AB - Modified wood fibres open new perspectives to create value-added products based on renewable raw materials. An interesting option is the targeted modification of fibre surfaces by oxidative enzymes. This two-stage functionalisation method consists of enzymatic activation of fibre surfaces followed by addition of radicalised compounds reacting preferentially by radical coupling. In this work, the activation of bleached and unbleached softwood TMPs with laccase isolated from Trametes hirsuta was studied. The formation and stability of the radicals were studied by EPR spectroscopy. The reaction of the radicals with 3-hydroxytyramine hydrochloride and the type of chemical linkages were investigated. EPR, ESCA and FTIR spectroscopy were used for analysis. Bleached TMP was radicalised more efficiently than unbleached TMP. The radicals were unstable, as 90% of them were quenched within a few hours. Their lifetime was, however, found to be adequately long for performing coupling reactions. Bonding of new compounds to pulps via radical reactions thus seems to be possible.

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Grönqvist S, Rantanen K, Alen R, Mattinen M-L, Buchert J, Viikari L. Laccase-catalysed functionalisation of TMP with tyramine. Holzforschung. 2006;60(5):503-508. https://doi.org/10.1515/HF.2006.083