Laccase-catalysed functionalisation of TMP with tyramine

Stina Grönqvist (Corresponding Author), K. Rantanen, Raimo Alen, Maija-Liisa Mattinen, Johanna Buchert, Liisa Viikari

    Research output: Contribution to journalArticleScientificpeer-review

    49 Citations (Scopus)


    Modified wood fibres open new perspectives to create value-added products based on renewable raw materials. An interesting option is the targeted modification of fibre surfaces by oxidative enzymes. This two-stage functionalisation method consists of enzymatic activation of fibre surfaces followed by addition of radicalised compounds reacting preferentially by radical coupling. In this work, the activation of bleached and unbleached softwood TMPs with laccase isolated from Trametes hirsuta was studied. The formation and stability of the radicals were studied by EPR spectroscopy. The reaction of the radicals with 3-hydroxytyramine hydrochloride and the type of chemical linkages were investigated. EPR, ESCA and FTIR spectroscopy were used for analysis. Bleached TMP was radicalised more efficiently than unbleached TMP. The radicals were unstable, as 90% of them were quenched within a few hours. Their lifetime was, however, found to be adequately long for performing coupling reactions. Bonding of new compounds to pulps via radical reactions thus seems to be possible.
    Original languageEnglish
    Pages (from-to)503-508
    Number of pages6
    Issue number5
    Publication statusPublished - 2006
    MoE publication typeA1 Journal article-refereed


    • Activation of fibres
    • EPR
    • ESCA (XPS)
    • FTIR
    • Functionalisation of fibres
    • Laccases
    • Lifetime of radicals
    • Surface modification


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