Laccase from Melanocarpus albomyces binds effectively to cellulose

Laura-Leena Kiiskinen, Hetti Palonen, Markus Linder, Liisa Viikari, Kristiina Kruus (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

21 Citations (Scopus)

Abstract

A laccase from the thermophilic fungus Melanocarpus albomyces was shown to bind to softwood and pure microcrystalline cellulose. The binding isotherm fitted well the Langmuir type one‐site binding model. The adsorption parameters indicated that M. albomyces laccase binds with high affinity to cellulose with a relatively low maximum binding capacity, as compared to the values for various cellulases. The binding was shown to be reversible and not influenced by non‐specific protein or 0.1–0.5 M Na2SO4. No binding was detected with laccases from Trametes hirsuta or Mauginiella sp., which suggests that binding to cellulose is typical for only some laccases.
Original languageEnglish
Pages (from-to)251 - 255
Number of pages5
JournalFEBS Letters
Volume576
Issue number1-2
DOIs
Publication statusPublished - 2004
MoE publication typeA1 Journal article-refereed

Fingerprint

Laccase
Cellulose
Trametes
Cellulases
Softwoods
Fungi
Adsorption
Isotherms
Proteins

Keywords

  • Cellulose binding
  • Bacterial microcrystalline cellulose
  • Melanocarpus albomyces laccase

Cite this

Kiiskinen, Laura-Leena ; Palonen, Hetti ; Linder, Markus ; Viikari, Liisa ; Kruus, Kristiina. / Laccase from Melanocarpus albomyces binds effectively to cellulose. In: FEBS Letters. 2004 ; Vol. 576, No. 1-2. pp. 251 - 255.
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abstract = "A laccase from the thermophilic fungus Melanocarpus albomyces was shown to bind to softwood and pure microcrystalline cellulose. The binding isotherm fitted well the Langmuir type one‐site binding model. The adsorption parameters indicated that M. albomyces laccase binds with high affinity to cellulose with a relatively low maximum binding capacity, as compared to the values for various cellulases. The binding was shown to be reversible and not influenced by non‐specific protein or 0.1–0.5 M Na2SO4. No binding was detected with laccases from Trametes hirsuta or Mauginiella sp., which suggests that binding to cellulose is typical for only some laccases.",
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Kiiskinen, L-L, Palonen, H, Linder, M, Viikari, L & Kruus, K 2004, 'Laccase from Melanocarpus albomyces binds effectively to cellulose', FEBS Letters, vol. 576, no. 1-2, pp. 251 - 255. https://doi.org/10.1016/j.febslet.2004.08.040

Laccase from Melanocarpus albomyces binds effectively to cellulose. / Kiiskinen, Laura-Leena; Palonen, Hetti; Linder, Markus; Viikari, Liisa; Kruus, Kristiina (Corresponding Author).

In: FEBS Letters, Vol. 576, No. 1-2, 2004, p. 251 - 255.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Laccase from Melanocarpus albomyces binds effectively to cellulose

AU - Kiiskinen, Laura-Leena

AU - Palonen, Hetti

AU - Linder, Markus

AU - Viikari, Liisa

AU - Kruus, Kristiina

PY - 2004

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AB - A laccase from the thermophilic fungus Melanocarpus albomyces was shown to bind to softwood and pure microcrystalline cellulose. The binding isotherm fitted well the Langmuir type one‐site binding model. The adsorption parameters indicated that M. albomyces laccase binds with high affinity to cellulose with a relatively low maximum binding capacity, as compared to the values for various cellulases. The binding was shown to be reversible and not influenced by non‐specific protein or 0.1–0.5 M Na2SO4. No binding was detected with laccases from Trametes hirsuta or Mauginiella sp., which suggests that binding to cellulose is typical for only some laccases.

KW - Cellulose binding

KW - Bacterial microcrystalline cellulose

KW - Melanocarpus albomyces laccase

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DO - 10.1016/j.febslet.2004.08.040

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SP - 251

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JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

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