Laccase from Melanocarpus albomyces binds effectively to cellulose

Laura-Leena Kiiskinen, Hetti Palonen, Markus Linder, Liisa Viikari, Kristiina Kruus (Corresponding Author)

    Research output: Contribution to journalArticleScientificpeer-review

    22 Citations (Scopus)

    Abstract

    A laccase from the thermophilic fungus Melanocarpus albomyces was shown to bind to softwood and pure microcrystalline cellulose. The binding isotherm fitted well the Langmuir type one‐site binding model. The adsorption parameters indicated that M. albomyces laccase binds with high affinity to cellulose with a relatively low maximum binding capacity, as compared to the values for various cellulases. The binding was shown to be reversible and not influenced by non‐specific protein or 0.1–0.5 M Na2SO4. No binding was detected with laccases from Trametes hirsuta or Mauginiella sp., which suggests that binding to cellulose is typical for only some laccases.
    Original languageEnglish
    Pages (from-to)251 - 255
    Number of pages5
    JournalFEBS Letters
    Volume576
    Issue number1-2
    DOIs
    Publication statusPublished - 2004
    MoE publication typeA1 Journal article-refereed

    Keywords

    • Cellulose binding
    • Bacterial microcrystalline cellulose
    • Melanocarpus albomyces laccase

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