Laccase from Melanocarpus albomyces binds effectively to cellulose

Laura-Leena Kiiskinen, Hetti Palonen, Markus Linder, Liisa Viikari, Kristiina Kruus (Corresponding Author)

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21 Citations (Scopus)

Abstract

A laccase from the thermophilic fungus Melanocarpus albomyces was shown to bind to softwood and pure microcrystalline cellulose. The binding isotherm fitted well the Langmuir type one‐site binding model. The adsorption parameters indicated that M. albomyces laccase binds with high affinity to cellulose with a relatively low maximum binding capacity, as compared to the values for various cellulases. The binding was shown to be reversible and not influenced by non‐specific protein or 0.1–0.5 M Na2SO4. No binding was detected with laccases from Trametes hirsuta or Mauginiella sp., which suggests that binding to cellulose is typical for only some laccases.
Original languageEnglish
Pages (from-to)251 - 255
Number of pages5
JournalFEBS Letters
Volume576
Issue number1-2
DOIs
Publication statusPublished - 2004
MoE publication typeA1 Journal article-refereed

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Keywords

  • Cellulose binding
  • Bacterial microcrystalline cellulose
  • Melanocarpus albomyces laccase

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