Laccase from the white-rot fungus Trametes versicolor

cDNA cloning of Icc1 and expression in Pichia pastoris

Research output: Contribution to journalArticleScientificpeer-review

116 Citations (Scopus)

Abstract

A cDNA coding for laccase was isolated from the ligninolytic fungus Trametes versicolor by RNA-PCR. The cDNA corresponds to the gene lcc1, which encodes a laccase isoenzyme of 498 amino-acid residues preceded by a 22-residue signal peptide. The lcc1 cDNA was cloned into the vector pHIL-D2 for expression in Pichia pastoris under the control of the AOX1 promoter. Transformants were found to secrete active recombinant enzyme after induction with methanol. The use of growth medium buffered to pH 6.0 and control of pH during cultivation were found to be important, or even necessary, for obtaining activity in liquid cultures. The effect of exchanging the native secretion signal for the Saccharomyces cerevisiae α-factor pre-pro secretion signal was studied by cloning the portion encoding the mature enzyme into the vector pPIC9. The activity obtained for the construct encoding the native laccase signal sequence was found to be seven-fold higher than for the construct encoding the a-factor secretion signal. Utilisation of the P. pastoris pep4 mutant strain SMD 1168 was found to provide a two-fold higher level of activity compared with P. pastoris GS115.

Original languageEnglish
Pages (from-to)425-430
Number of pages6
JournalCurrent Genetics
Volume32
Issue number6
DOIs
Publication statusPublished - 1 Dec 1997
MoE publication typeA1 Journal article-refereed

Fingerprint

Trametes
Laccase
Pichia
Organism Cloning
Fungi
Complementary DNA
Protein Sorting Signals
Enzyme Induction
Isoenzymes
Methanol
Saccharomyces cerevisiae
RNA
Amino Acids
Polymerase Chain Reaction
Enzymes
Growth
Genes

Keywords

  • Heterologous expression
  • Laccase
  • Pichia pastoris
  • Trametes versicolor

Cite this

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title = "Laccase from the white-rot fungus Trametes versicolor: cDNA cloning of Icc1 and expression in Pichia pastoris",
abstract = "A cDNA coding for laccase was isolated from the ligninolytic fungus Trametes versicolor by RNA-PCR. The cDNA corresponds to the gene lcc1, which encodes a laccase isoenzyme of 498 amino-acid residues preceded by a 22-residue signal peptide. The lcc1 cDNA was cloned into the vector pHIL-D2 for expression in Pichia pastoris under the control of the AOX1 promoter. Transformants were found to secrete active recombinant enzyme after induction with methanol. The use of growth medium buffered to pH 6.0 and control of pH during cultivation were found to be important, or even necessary, for obtaining activity in liquid cultures. The effect of exchanging the native secretion signal for the Saccharomyces cerevisiae α-factor pre-pro secretion signal was studied by cloning the portion encoding the mature enzyme into the vector pPIC9. The activity obtained for the construct encoding the native laccase signal sequence was found to be seven-fold higher than for the construct encoding the a-factor secretion signal. Utilisation of the P. pastoris pep4 mutant strain SMD 1168 was found to provide a two-fold higher level of activity compared with P. pastoris GS115.",
keywords = "Heterologous expression, Laccase, Pichia pastoris, Trametes versicolor",
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Laccase from the white-rot fungus Trametes versicolor : cDNA cloning of Icc1 and expression in Pichia pastoris. / Jönsson, Leif J.; Saloheimo, Markku; Penttilä, Merja.

In: Current Genetics, Vol. 32, No. 6, 01.12.1997, p. 425-430.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

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AU - Jönsson, Leif J.

AU - Saloheimo, Markku

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AB - A cDNA coding for laccase was isolated from the ligninolytic fungus Trametes versicolor by RNA-PCR. The cDNA corresponds to the gene lcc1, which encodes a laccase isoenzyme of 498 amino-acid residues preceded by a 22-residue signal peptide. The lcc1 cDNA was cloned into the vector pHIL-D2 for expression in Pichia pastoris under the control of the AOX1 promoter. Transformants were found to secrete active recombinant enzyme after induction with methanol. The use of growth medium buffered to pH 6.0 and control of pH during cultivation were found to be important, or even necessary, for obtaining activity in liquid cultures. The effect of exchanging the native secretion signal for the Saccharomyces cerevisiae α-factor pre-pro secretion signal was studied by cloning the portion encoding the mature enzyme into the vector pPIC9. The activity obtained for the construct encoding the native laccase signal sequence was found to be seven-fold higher than for the construct encoding the a-factor secretion signal. Utilisation of the P. pastoris pep4 mutant strain SMD 1168 was found to provide a two-fold higher level of activity compared with P. pastoris GS115.

KW - Heterologous expression

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