Abstract
Dextran-producing Weissella have received significant
attention. However, except for maltose, the acceptor
reactions of Weissella dextransucrases with different
sugars have not been investigated. The action of
recombinant Weissella confusa VTT E-90392 dextransucrase
was tested with several potential acceptors,
particularly, analogs lactose and cellobiose. The major
acceptor products of both disaccharides were identified
as branched trisaccharides, with a glucosyl residue
[alpha]-(1->2)-linked to the acceptor's reducing end. An
additional product, isomelezitose
(6Fru-[alpha]-Glcp-sucrose), was also produced when using
lactose as an acceptor. This is the first report of the
synthesis of isomelezitose by a dextransucrase. The NMR
spectra of the three trisaccharides were fully assigned,
and their structures were confirmed by selective
enzymatic hydrolysis. The trisaccharides prepared from
13C6glc sucrose and lactose were analyzed by ESI-MSn, and
the fragmentation patterns of these compounds were
characterized.
Original language | English |
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Pages (from-to) | 226-236 |
Journal | Food Chemistry |
Volume | 190 |
DOIs | |
Publication status | Published - 2016 |
MoE publication type | A1 Journal article-refereed |
Keywords
- Dextransucrase
- Oligosaccharide
- Weissella
- Isomelezitose
- Tandem mass spectrometry
- NMR