Laser-induced fluorescence (LIF) of lignin and lignin model compounds in Raman spectroscopy

A. Lähdetie (Corresponding Author), P. Nousiainen, J. Sipilä, Tarja Tamminen, Anna-Stiina Jääskeläinen

Research output: Contribution to journalArticleScientificpeer-review

8 Citations (Scopus)

Abstract

Raman spectroscopy is a technique that provides structural information on lignin and other components of wood and pulp in situ. However, especially lignin-containing samples may produce laser-induced fluorescence (LIF) that overlaps with Raman bands. In the worst case, this background signal can overwhelm the weaker Raman signal completely. In this study, the LIF of lignin was investigated with the excitation wavelength 532 nm applied in Raman spectroscopy to clarify the correlations between lignin structure and LIF intensity. Raman spectroscopic analyses with lignin model compounds illustrated that the 5-5′ structures induce LIF. It was also shown that the intensity of LIF was significantly less intense when the 5-5′ model compound was structurally rigid (as in dibenzodioxocin) compared with the flexible simple counterpart. The comparison between the free phenolic model compounds with the methylated analogue showed that the presence of the free phenolic structure was not a prerequisite for LIF. It was thus concluded that the conformation of the molecule is the key factor with respect to fluorescence. The role of conformational aspects was further investigated by comparing wood with chemical pulps and isolated lignins.
Original languageEnglish
Pages (from-to)531-538
Number of pages7
JournalHolzforschung
Volume67
Issue number5
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

Fingerprint

Lignin
Raman spectroscopy
Fluorescence
Lasers
Wood
Chemical pulp
Pulp
Conformations
Wavelength
Molecules

Keywords

  • 5-5' linkage
  • dibenzodioxocin
  • fluorescence spectroscopy
  • laser-induced fluorescence (LIF)
  • lignin
  • Raman spectroscopy

Cite this

Lähdetie, A. ; Nousiainen, P. ; Sipilä, J. ; Tamminen, Tarja ; Jääskeläinen, Anna-Stiina. / Laser-induced fluorescence (LIF) of lignin and lignin model compounds in Raman spectroscopy. In: Holzforschung. 2013 ; Vol. 67, No. 5. pp. 531-538.
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abstract = "Raman spectroscopy is a technique that provides structural information on lignin and other components of wood and pulp in situ. However, especially lignin-containing samples may produce laser-induced fluorescence (LIF) that overlaps with Raman bands. In the worst case, this background signal can overwhelm the weaker Raman signal completely. In this study, the LIF of lignin was investigated with the excitation wavelength 532 nm applied in Raman spectroscopy to clarify the correlations between lignin structure and LIF intensity. Raman spectroscopic analyses with lignin model compounds illustrated that the 5-5′ structures induce LIF. It was also shown that the intensity of LIF was significantly less intense when the 5-5′ model compound was structurally rigid (as in dibenzodioxocin) compared with the flexible simple counterpart. The comparison between the free phenolic model compounds with the methylated analogue showed that the presence of the free phenolic structure was not a prerequisite for LIF. It was thus concluded that the conformation of the molecule is the key factor with respect to fluorescence. The role of conformational aspects was further investigated by comparing wood with chemical pulps and isolated lignins.",
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Lähdetie, A, Nousiainen, P, Sipilä, J, Tamminen, T & Jääskeläinen, A-S 2013, 'Laser-induced fluorescence (LIF) of lignin and lignin model compounds in Raman spectroscopy', Holzforschung, vol. 67, no. 5, pp. 531-538. https://doi.org/10.1515/hf-2012-0177

Laser-induced fluorescence (LIF) of lignin and lignin model compounds in Raman spectroscopy. / Lähdetie, A. (Corresponding Author); Nousiainen, P.; Sipilä, J.; Tamminen, Tarja; Jääskeläinen, Anna-Stiina.

In: Holzforschung, Vol. 67, No. 5, 2013, p. 531-538.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Laser-induced fluorescence (LIF) of lignin and lignin model compounds in Raman spectroscopy

AU - Lähdetie, A.

AU - Nousiainen, P.

AU - Sipilä, J.

AU - Tamminen, Tarja

AU - Jääskeläinen, Anna-Stiina

PY - 2013

Y1 - 2013

N2 - Raman spectroscopy is a technique that provides structural information on lignin and other components of wood and pulp in situ. However, especially lignin-containing samples may produce laser-induced fluorescence (LIF) that overlaps with Raman bands. In the worst case, this background signal can overwhelm the weaker Raman signal completely. In this study, the LIF of lignin was investigated with the excitation wavelength 532 nm applied in Raman spectroscopy to clarify the correlations between lignin structure and LIF intensity. Raman spectroscopic analyses with lignin model compounds illustrated that the 5-5′ structures induce LIF. It was also shown that the intensity of LIF was significantly less intense when the 5-5′ model compound was structurally rigid (as in dibenzodioxocin) compared with the flexible simple counterpart. The comparison between the free phenolic model compounds with the methylated analogue showed that the presence of the free phenolic structure was not a prerequisite for LIF. It was thus concluded that the conformation of the molecule is the key factor with respect to fluorescence. The role of conformational aspects was further investigated by comparing wood with chemical pulps and isolated lignins.

AB - Raman spectroscopy is a technique that provides structural information on lignin and other components of wood and pulp in situ. However, especially lignin-containing samples may produce laser-induced fluorescence (LIF) that overlaps with Raman bands. In the worst case, this background signal can overwhelm the weaker Raman signal completely. In this study, the LIF of lignin was investigated with the excitation wavelength 532 nm applied in Raman spectroscopy to clarify the correlations between lignin structure and LIF intensity. Raman spectroscopic analyses with lignin model compounds illustrated that the 5-5′ structures induce LIF. It was also shown that the intensity of LIF was significantly less intense when the 5-5′ model compound was structurally rigid (as in dibenzodioxocin) compared with the flexible simple counterpart. The comparison between the free phenolic model compounds with the methylated analogue showed that the presence of the free phenolic structure was not a prerequisite for LIF. It was thus concluded that the conformation of the molecule is the key factor with respect to fluorescence. The role of conformational aspects was further investigated by comparing wood with chemical pulps and isolated lignins.

KW - 5-5' linkage

KW - dibenzodioxocin

KW - fluorescence spectroscopy

KW - laser-induced fluorescence (LIF)

KW - lignin

KW - Raman spectroscopy

U2 - 10.1515/hf-2012-0177

DO - 10.1515/hf-2012-0177

M3 - Article

VL - 67

SP - 531

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JO - Holzforschung

JF - Holzforschung

SN - 0018-3830

IS - 5

ER -