Layer formation of a lipid-taged single-chain antibody and the interaction with antigen

Inger Vikholm (Corresponding Author), Jouko Peltonen

Research output: Contribution to journalArticleScientificpeer-review

7 Citations (Scopus)

Abstract

A phospholipid monolayer was prepared at the air-water interface and different amounts of a lipid-tagged genetically fragmented antibody were incorporated into the lipid matrix. The molecular surface area of the film increased with antibody concentration and reached a maximum at an antibody mole fraction of 0.03. Atomic force microscopy showed that the antibody fragment was segregated into protein-rich domains with a mean diameter of about 12 nm. In-situ measurements with surface plasmon resonance displayed a specific binding of antigen—a binding that was not observed to the pure lipid film.
Original languageEnglish
Pages (from-to)924-926
JournalThin Solid Films
Volume284-285
DOIs
Publication statusPublished - 1996
MoE publication typeA1 Journal article-refereed
EventSeventh International Conference on Organized Molecular Films - Numana, Italy
Duration: 10 Sep 199515 Sep 1995

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