Layer formation of a lipid-taged single-chain antibody and the interaction with antigen

Inger Vikholm (Corresponding Author), Jouko Peltonen

Research output: Contribution to journalArticleScientificpeer-review

7 Citations (Scopus)

Abstract

A phospholipid monolayer was prepared at the air-water interface and different amounts of a lipid-tagged genetically fragmented antibody were incorporated into the lipid matrix. The molecular surface area of the film increased with antibody concentration and reached a maximum at an antibody mole fraction of 0.03. Atomic force microscopy showed that the antibody fragment was segregated into protein-rich domains with a mean diameter of about 12 nm. In-situ measurements with surface plasmon resonance displayed a specific binding of antigen—a binding that was not observed to the pure lipid film.
Original languageEnglish
Pages (from-to)924-926
Number of pages3
JournalThin Solid Films
Volume284-285
DOIs
Publication statusPublished - 1996
MoE publication typeA1 Journal article-refereed
EventSeventh International Conference on Organized Molecular Films - Numana, Italy
Duration: 10 Sep 199515 Sep 1995

Fingerprint

Single-Chain Antibodies
antigens
Antigens
antibodies
Antibodies
Lipids
lipids
Immunoglobulin Fragments
Surface plasmon resonance
interactions
Monolayers
Atomic force microscopy
Phospholipids
in situ measurement
surface plasmon resonance
Water
fragments
atomic force microscopy
Air
proteins

Cite this

Vikholm, Inger ; Peltonen, Jouko. / Layer formation of a lipid-taged single-chain antibody and the interaction with antigen. In: Thin Solid Films. 1996 ; Vol. 284-285. pp. 924-926.
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Layer formation of a lipid-taged single-chain antibody and the interaction with antigen. / Vikholm, Inger (Corresponding Author); Peltonen, Jouko.

In: Thin Solid Films, Vol. 284-285, 1996, p. 924-926.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Layer formation of a lipid-taged single-chain antibody and the interaction with antigen

AU - Vikholm, Inger

AU - Peltonen, Jouko

N1 - Project code: KET4245

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AB - A phospholipid monolayer was prepared at the air-water interface and different amounts of a lipid-tagged genetically fragmented antibody were incorporated into the lipid matrix. The molecular surface area of the film increased with antibody concentration and reached a maximum at an antibody mole fraction of 0.03. Atomic force microscopy showed that the antibody fragment was segregated into protein-rich domains with a mean diameter of about 12 nm. In-situ measurements with surface plasmon resonance displayed a specific binding of antigen—a binding that was not observed to the pure lipid film.

U2 - 10.1016/S0040-6090(95)08481-9

DO - 10.1016/S0040-6090(95)08481-9

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VL - 284-285

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EP - 926

JO - Thin Solid Films

JF - Thin Solid Films

SN - 0040-6090

ER -