Abstract
Limited hydrolysis of rice endosperm protein isolate was carried out with acid and neutral endoproteases to evaluate the relationship between degree of hydrolysis and techno-functional properties. The highest studied degree of hydrolysis was 5.4% corresponding to 55.2% protein solubility. Solubility increased as a function of degree of hydrolysis with higher efficiency by acid endoprotease. Colloidal stability of the protein suspensions steadily increased with increasing degree of hydrolysis. Higher colloidal stability values were achieved by neutral endoprotease (31–89%) compared to that by acid endoprotease (20–75%). On the other hand, the absolute values of zeta potential and surface hydrophobicity decreased as a function of degree of hydrolysis leading to higher values by neutral endoprotease (−21.4 mV and 21.7 mV) than by acid endoprotease (−813.4 mV and 11.7 mV). Foaming, gel formation and water holding properties improved only until degree of hydrolysis values of 1.5% (neural endoprotease) and 1.9% (acid endoprotease).
| Original language | English |
|---|---|
| Article number | 125274 |
| Journal | Food Chemistry |
| Volume | 302 |
| Early online date | 27 Jul 2019 |
| DOIs | |
| Publication status | Published - 1 Jan 2020 |
| MoE publication type | A1 Journal article-refereed |
Funding
This study was supported by the funding from the Bio Based industries Joint Undertaking under the European Union’s Horizon 2020 research and innovation programme under grant agreement No 668953-PROMINENT . The authors gratefully acknowledge Eero Mattila, Leila Kostamo and Anna-Liisa Ruskeepää for their assistance in the experimental work.
Keywords
- Degree of hydrolysis
- Functional properties
- Limited enzymatic hydrolysis
- Rice endosperm protein