Link between plasma ceramides, inflammation and insulin resistance: Association with serum IL-6 concentration in patients with coronary heart disease

V.D.F. de Mello (Corresponding Author), Maria Lankinen, U. Schwab, M. Kolehmainen, S. Lehto, Tuulikki Seppänen-Laakso, Matej Oresic, L. Pulkkinen, M. Uusitupa, A.T. Erkkilä

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Abstract

Aims/hypothesis: Ceramides and IL-6 have a role in immune–inflammatory responses and cardiovascular diseases, and are suggested to be involved in insulin and glucose metabolism. We sought to assess the associations of circulating levels of IL-6, TNF-α and high-sensitivity C reactive protein (hsCRP), which are inflammatory markers related to insulin resistance (IR), with the plasma lipid metabolites ceramides and diacylglycerols (DAG) in patients with CHD. Methods: Cross-sectional analyses were carried out on data from 33 patients with CHD. Serum levels of the inflammatory markers and plasma lipid metabolites (lipidomics approach performed by ultra-performance liquid chromatography coupled to electrospray ionisation MS) were measured at the same time point as insulin resistance (IR) (HOMA-IR index). Results: Serum circulating levels of IL-6 were strongly correlated with plasma ceramide concentrations (r = 0.59, p < 0.001). Adjustments for serum TNF-α or hsCRP levels, smoking, BMI, age, sex or HOMA-IR did not change the results (p < 0.001). After adjustments for the effect of serum inflammatory markers (TNF-α or hsCRP), HOMA-IR and BMI the correlation between plasma DAG and serum IL-6 (r = 0.33) was also significant (p < 0.03). In a linear regression model, circulating levels of both ceramides and TNF-α had a significant independent influence on circulating levels of IL-6, altogether accounting for 41% of its variation (p < 0.001). Conclusions/interpretation: Our results strongly suggest that the link between ceramides, IR and inflammation is related to the inflammatory marker IL-6. Ceramides may contribute to the induction of inflammation involved in IR states that frequently coexist with CHD.
Original languageEnglish
Pages (from-to)2612-2615
Number of pages4
JournalDiabetologia
Volume52
Issue number12
DOIs
Publication statusPublished - 2009
MoE publication typeA1 Journal article-refereed

Fingerprint

Ceramides
Coronary Disease
Insulin Resistance
Interleukin-6
Inflammation
Serum
C-Reactive Protein
Diglycerides
Linear Models
Lipids
Liquid Chromatography
Cardiovascular Diseases
Cross-Sectional Studies
Biomarkers
Smoking
Insulin
Glucose

Keywords

  • cardiovascular disease
  • diacylglycerols
  • IL-6
  • inflammation
  • insulin resistance
  • lipidomics

Cite this

de Mello, V.D.F. ; Lankinen, Maria ; Schwab, U. ; Kolehmainen, M. ; Lehto, S. ; Seppänen-Laakso, Tuulikki ; Oresic, Matej ; Pulkkinen, L. ; Uusitupa, M. ; Erkkilä, A.T. / Link between plasma ceramides, inflammation and insulin resistance : Association with serum IL-6 concentration in patients with coronary heart disease. In: Diabetologia. 2009 ; Vol. 52, No. 12. pp. 2612-2615.
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abstract = "Aims/hypothesis: Ceramides and IL-6 have a role in immune–inflammatory responses and cardiovascular diseases, and are suggested to be involved in insulin and glucose metabolism. We sought to assess the associations of circulating levels of IL-6, TNF-α and high-sensitivity C reactive protein (hsCRP), which are inflammatory markers related to insulin resistance (IR), with the plasma lipid metabolites ceramides and diacylglycerols (DAG) in patients with CHD. Methods: Cross-sectional analyses were carried out on data from 33 patients with CHD. Serum levels of the inflammatory markers and plasma lipid metabolites (lipidomics approach performed by ultra-performance liquid chromatography coupled to electrospray ionisation MS) were measured at the same time point as insulin resistance (IR) (HOMA-IR index). Results: Serum circulating levels of IL-6 were strongly correlated with plasma ceramide concentrations (r = 0.59, p < 0.001). Adjustments for serum TNF-α or hsCRP levels, smoking, BMI, age, sex or HOMA-IR did not change the results (p < 0.001). After adjustments for the effect of serum inflammatory markers (TNF-α or hsCRP), HOMA-IR and BMI the correlation between plasma DAG and serum IL-6 (r = 0.33) was also significant (p < 0.03). In a linear regression model, circulating levels of both ceramides and TNF-α had a significant independent influence on circulating levels of IL-6, altogether accounting for 41{\%} of its variation (p < 0.001). Conclusions/interpretation: Our results strongly suggest that the link between ceramides, IR and inflammation is related to the inflammatory marker IL-6. Ceramides may contribute to the induction of inflammation involved in IR states that frequently coexist with CHD.",
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de Mello, VDF, Lankinen, M, Schwab, U, Kolehmainen, M, Lehto, S, Seppänen-Laakso, T, Oresic, M, Pulkkinen, L, Uusitupa, M & Erkkilä, AT 2009, 'Link between plasma ceramides, inflammation and insulin resistance: Association with serum IL-6 concentration in patients with coronary heart disease', Diabetologia, vol. 52, no. 12, pp. 2612-2615. https://doi.org/10.1007/s00125-009-1482-9

Link between plasma ceramides, inflammation and insulin resistance : Association with serum IL-6 concentration in patients with coronary heart disease. / de Mello, V.D.F. (Corresponding Author); Lankinen, Maria; Schwab, U.; Kolehmainen, M.; Lehto, S.; Seppänen-Laakso, Tuulikki; Oresic, Matej; Pulkkinen, L.; Uusitupa, M.; Erkkilä, A.T.

In: Diabetologia, Vol. 52, No. 12, 2009, p. 2612-2615.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Link between plasma ceramides, inflammation and insulin resistance

T2 - Association with serum IL-6 concentration in patients with coronary heart disease

AU - de Mello, V.D.F.

AU - Lankinen, Maria

AU - Schwab, U.

AU - Kolehmainen, M.

AU - Lehto, S.

AU - Seppänen-Laakso, Tuulikki

AU - Oresic, Matej

AU - Pulkkinen, L.

AU - Uusitupa, M.

AU - Erkkilä, A.T.

PY - 2009

Y1 - 2009

N2 - Aims/hypothesis: Ceramides and IL-6 have a role in immune–inflammatory responses and cardiovascular diseases, and are suggested to be involved in insulin and glucose metabolism. We sought to assess the associations of circulating levels of IL-6, TNF-α and high-sensitivity C reactive protein (hsCRP), which are inflammatory markers related to insulin resistance (IR), with the plasma lipid metabolites ceramides and diacylglycerols (DAG) in patients with CHD. Methods: Cross-sectional analyses were carried out on data from 33 patients with CHD. Serum levels of the inflammatory markers and plasma lipid metabolites (lipidomics approach performed by ultra-performance liquid chromatography coupled to electrospray ionisation MS) were measured at the same time point as insulin resistance (IR) (HOMA-IR index). Results: Serum circulating levels of IL-6 were strongly correlated with plasma ceramide concentrations (r = 0.59, p < 0.001). Adjustments for serum TNF-α or hsCRP levels, smoking, BMI, age, sex or HOMA-IR did not change the results (p < 0.001). After adjustments for the effect of serum inflammatory markers (TNF-α or hsCRP), HOMA-IR and BMI the correlation between plasma DAG and serum IL-6 (r = 0.33) was also significant (p < 0.03). In a linear regression model, circulating levels of both ceramides and TNF-α had a significant independent influence on circulating levels of IL-6, altogether accounting for 41% of its variation (p < 0.001). Conclusions/interpretation: Our results strongly suggest that the link between ceramides, IR and inflammation is related to the inflammatory marker IL-6. Ceramides may contribute to the induction of inflammation involved in IR states that frequently coexist with CHD.

AB - Aims/hypothesis: Ceramides and IL-6 have a role in immune–inflammatory responses and cardiovascular diseases, and are suggested to be involved in insulin and glucose metabolism. We sought to assess the associations of circulating levels of IL-6, TNF-α and high-sensitivity C reactive protein (hsCRP), which are inflammatory markers related to insulin resistance (IR), with the plasma lipid metabolites ceramides and diacylglycerols (DAG) in patients with CHD. Methods: Cross-sectional analyses were carried out on data from 33 patients with CHD. Serum levels of the inflammatory markers and plasma lipid metabolites (lipidomics approach performed by ultra-performance liquid chromatography coupled to electrospray ionisation MS) were measured at the same time point as insulin resistance (IR) (HOMA-IR index). Results: Serum circulating levels of IL-6 were strongly correlated with plasma ceramide concentrations (r = 0.59, p < 0.001). Adjustments for serum TNF-α or hsCRP levels, smoking, BMI, age, sex or HOMA-IR did not change the results (p < 0.001). After adjustments for the effect of serum inflammatory markers (TNF-α or hsCRP), HOMA-IR and BMI the correlation between plasma DAG and serum IL-6 (r = 0.33) was also significant (p < 0.03). In a linear regression model, circulating levels of both ceramides and TNF-α had a significant independent influence on circulating levels of IL-6, altogether accounting for 41% of its variation (p < 0.001). Conclusions/interpretation: Our results strongly suggest that the link between ceramides, IR and inflammation is related to the inflammatory marker IL-6. Ceramides may contribute to the induction of inflammation involved in IR states that frequently coexist with CHD.

KW - cardiovascular disease

KW - diacylglycerols

KW - IL-6

KW - inflammation

KW - insulin resistance

KW - lipidomics

U2 - 10.1007/s00125-009-1482-9

DO - 10.1007/s00125-009-1482-9

M3 - Article

VL - 52

SP - 2612

EP - 2615

JO - Diabetologia

JF - Diabetologia

SN - 0012-186X

IS - 12

ER -